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Straighterline | BIO250LLab 7 Microbial Genetics & Genetic Engineering | latest updated version with 100-

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Straighterline | BIO250LLab 7 Microbial Genetics & Genetic Engineering | latest updated version with 100-

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Straighterline
Course
BIO250L

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Straighterline
BIO250LLab 7 Microbial
Genetics & Genetic
Engineering latest
updated version with
100% accurate solutions
Student Name: Hope Amodu
Access Code (located on the lid of your lab kit): Lp-01010466131


Lab Report Format Expectations
Utilize college level grammar and professional formatting when completing this
worksheet.
Submissions without proper formatting, all required photos or sufficient responses
will be rejected.
Pre-lab Questions
1. Why are the concepts in Lab 7 important in the field of microbiology?
These ideas are important because they help scientists do research in
microbiology and medicine.

2. Which DNA nitrogenous bases pair with each other?

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Adenine (A) pairs with Thymine (T) using two bonds. Guanine (G)
pairs with Cytosine (C) using three bonds.

3. Which bases are purines, and which are pyrimidines?
Adenine and guanine are purines. Cytosine and thymine (or uracil in
RNA) are pyrimidines.

4. How is DNA information used to make proteins? What are the steps
of this process, and what are the significant pieces of cell machinery
that participate in this process?
DNA makes proteins in two steps: transcription and translation. In
transcription, RNA polymerase copies DNA into mRNA. In
translation, ribosomes read the mRNA, and tRNA brings the right
amino acids. These amino acids link together to make proteins.

5. What occurs during each of the three steps involved in the PCR
cycle? How has the use of PCR changed biotechnology?
PCR, or Polymerase Chain Reaction, has three main steps:
denaturation, annealing, and extension. In denaturation, heat is used
to separate the two DNA strands. During annealing, the temperature
is lowered so primers can attach to the DNA. In extension, new DNA
bases are added to build matching strands. This process is important
because it allows scientists to make many copies of DNA for
research, medicine, and solving crimes.

6. What are the three-nucleotide sequences that correspond to a specific
amino acid called? A codon is a set of three DNA or RNA letters that
code for an amino acid or tell the cell to start/stop making a protein.
There are 64 codons, 61 for amino acids and 3 for stop signals.

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