BIOCHEM C785 OA readiness check Western University

What is the complementary sequence to 3’ ATG CGA ATA 5’? (0/1 Points) 3’ TAT TCG CAT 5’ 3’ TAC GCT TAT 5’ The correct answer is 3' TAT TCG CAT 5'. Remember complementary means “Matching or Pairing” You have to remember to pay attention to your numbers as well as your letters (A-T, G-C, 5'-3'). The correct answer is 3’ ATG CGA ATA 5’ (original sequence) 5’ TAC GCT TAT 3’ (complementary sequence) But we asked for it in the 3’ direction, so mirror answer to give correct answer: 3’ TAT TCG CAT 5’ If you chose 3' TAC GCT TAT 5', this is incorrect. Although the nucleotides are in the correct order, the 5' and 3' are in the wrong place. DNA strands that are complementary run in opposite directions. This means that if one strand starts with a 3', the the complementary strand must start with a 5'. If none of the answer choices give the correct answer in 5' ---> 3', then you need to write its mirror, which will run from 3' ---> 5'. 3’ GAT AGC ATA 5’ 5’ ATA AGC GTA 3’ 2 Which amino acid sequence would result from this Coding DNA strand? 3’ ATG CAG ATA 5’? (1/1 Points) Tyr Val Tyr Ile Gln Ile Ile Asp Val The correct answer is Ile Asp Val. We are starting at the coding strand, and have to remember the relationship between coding DNA and mRNA. These two strands are noncomplementary and parallel. So we copy the coding strand , change T ---> U, and then write the mRNA sequence: 3’ ATG CAG ATA 5’ coding 3’ AUG CAG AUA 5’ mRNAMirror by changing orientation: 5’ AUA GAC GUA 3’ Read chart Ile Asp Val (chart is in direction of 5' ---> 3') Leu His Lys 3 Which amino acid sequence would be made from this template DNA strand? 5’ TAT TAC CGA 3’? (1/1 Points) Tyr Tyr Arg Ile Met Ala Ser Val Ile The correct answer is Ser Val Ile because 5’ TAT TAC CGA 3’ template is complementary and antiparallel so 3’ AUA AUG GCU 5’ but it is in the wrong orientation, so mirror 5’ UCG GUA AUA 3’ and read the chart Ser Val Ile Ser His Gln 4 Which of the following are the correct components for a PCR reaction? (1/1 Points) dNTPs, Primer, RNA Polymerase, template RNA dNTPs, Primer, DNA Polymerase, template DNA The correct answer is dNTPs, Primer, DNA Polymerase, template DNA. Notice that all components are about DNA. The Primer is even a DNA Primer. ATP, Primer, mRNA polymerase; template mRNA Acetyl CoA, RNA primer, DNA Ligase, Template phosphate 5 Assuming 100% reaction efficiency, how many DNA copies will be produced after 5 PCR cycles, if we begin with 1 DNA template? (1/1 Points) 16 32 The correct answer is 32. 2x2x2x2x2=32 64 10 6 Which of the following would represent a silent mutation if this is the original sequence: 5’ AUC GUA ACA 3’? (1/1 Points) 5’ AUC GGA ACA 3’ 5' AUA GUA ACA 3' The correct answer is 5' AUC GUA ACA 3' Ile Val Thr 5’ AUG GUA ACA 3’ 5’ AUC GCA ACA 3’ 7 If the original coding sequence is 5’ CGA TAC TTC AGA 3’ and it is mutated to 5' CGA TAT TTC AGA 3', what type of mutation would have taken place? (1/1 Points) Silent The correct answer is silent mutation. The nucleotide sequence changes, but it codes for the same amino acid. The coding sequence 5' TAC 3' corresponds to the mRNA sequence 5' UAC 3' (Tyr), and the coding sequence 5' TAT 3' corresponds to the mRNA sequence 5' UAU 3' (Tyr). Since the C changed to at T, this is a point mutation. If the point mutation results in the same amino acid in the new sequence as in the original sequence, the point mutation is a sil