ANSWERS|LATEST !!!!2026|GRADED A+| (EXAM
A nanometer is defined as: - ANSWER 10 -9 or one-billionth of a meter
True or False: A nanometer is longer than a micrometer - ANSWER False
A nanometer is 1000x smaller than a micrometer
Resolution and contrast are two critical factors that influence your ability to see an object.
Explain each. - ANSWER Resolution refers to the distance between two objects at
which the objects can still be seen as separate. Poor or low resolution means two or more
objects may appear as one.
Contrast on the other hand is the difference in light absorbance between two objects. Poor
contrast gives a high background and makes the visualization of multiple objects difficult. For
instance, trying to identify 2 dark colored objects at
night (low light = low contrast) versus the same 2 objects in the middle of
a sunny afternoon (bright light against 2 dark objects = high contrast).
Assuming a fixed ocular, identify the part of the microscope you would adjust to enhance the
magnification of a sample. - ANSWER Objective
Only the oculars (eyepiece) and the objectives contribute to the magnification of the
sample. Since the eyepiece
is fixed, only the objectives could be altered.
1
, Assuming a constant (non-adjustable) light source power, identify the part of the microscope
you would adjust to limit the amount of light entering the microscope. -
ANSWER Iris diaphragm
What is the total magnification on (relative to your eye) of a sample imaged with a 60x
objective and a 10x eyepiece? Show your math. - ANSWER 60x objective x 10x
ocular = 600x magnification
True or False: Staining is often required to image a cell that is adherent and flat (thin). -
ANSWER True
Adherent, flat and unstained cells are almost invisible due to the limits on both
resolution and contrast. Therefore, cell staining is often required to adequately image
the sample.
True or False: A cell that is adherent, flat (thin), and unstained is easily identified using bright
field microscopy - ANSWER False: Adherent, flat cells are almost invisible due to the
limits on both resolution and contrast
Which of the following could be seen clearly by the unaided eye? Select all that apply.
A. Bacteria with diameter of 24 μm
B. Protozoa with diameter of 150 μm
C. Virus with a diameter of 0.2 μm
D. Skin cell with diameter of 1500 μm - ANSWER B. Protozoa with diameter of 150
μm
D. Skin cell with diameter of 1500 μm
2
A nanometer is defined as: - ANSWER 10 -9 or one-billionth of a meter
True or False: A nanometer is longer than a micrometer - ANSWER False
A nanometer is 1000x smaller than a micrometer
Resolution and contrast are two critical factors that influence your ability to see an object.
Explain each. - ANSWER Resolution refers to the distance between two objects at
which the objects can still be seen as separate. Poor or low resolution means two or more
objects may appear as one.
Contrast on the other hand is the difference in light absorbance between two objects. Poor
contrast gives a high background and makes the visualization of multiple objects difficult. For
instance, trying to identify 2 dark colored objects at
night (low light = low contrast) versus the same 2 objects in the middle of
a sunny afternoon (bright light against 2 dark objects = high contrast).
Assuming a fixed ocular, identify the part of the microscope you would adjust to enhance the
magnification of a sample. - ANSWER Objective
Only the oculars (eyepiece) and the objectives contribute to the magnification of the
sample. Since the eyepiece
is fixed, only the objectives could be altered.
1
, Assuming a constant (non-adjustable) light source power, identify the part of the microscope
you would adjust to limit the amount of light entering the microscope. -
ANSWER Iris diaphragm
What is the total magnification on (relative to your eye) of a sample imaged with a 60x
objective and a 10x eyepiece? Show your math. - ANSWER 60x objective x 10x
ocular = 600x magnification
True or False: Staining is often required to image a cell that is adherent and flat (thin). -
ANSWER True
Adherent, flat and unstained cells are almost invisible due to the limits on both
resolution and contrast. Therefore, cell staining is often required to adequately image
the sample.
True or False: A cell that is adherent, flat (thin), and unstained is easily identified using bright
field microscopy - ANSWER False: Adherent, flat cells are almost invisible due to the
limits on both resolution and contrast
Which of the following could be seen clearly by the unaided eye? Select all that apply.
A. Bacteria with diameter of 24 μm
B. Protozoa with diameter of 150 μm
C. Virus with a diameter of 0.2 μm
D. Skin cell with diameter of 1500 μm - ANSWER B. Protozoa with diameter of 150
μm
D. Skin cell with diameter of 1500 μm
2
