MLS 413 Final Exam Study Guide Questions With
Complete Solutions
If all the screen cell lines are negative for the AHG phase and
check cells what phases of the crossmatch procedure need to
performed?
Only IS
When must all phases of the cross match procedure be
performed?
When the screen cell lines have a reaction during the AHG
phase?
When working on solving an antibody panel which of the
following steps would be performed first?
Find the first cell line with a negative reaction at AHG and
perform homozygous cross outs.
How do you determine how many type specific donor units
would need to be antigen typed in order to fun a specific number
of compatible units?
Amount of units needed/Negative antigen incedence
If multiple antibodies: amount of units need/(antibody 1-
negative antigen incidence * antibody 2-negative antigen
incidence)
What would be the proper ABO group selection order for a B
positive recipient?
,B pos, B neg, O pos, O neg
Which Rh antigen pair would be considered heterozygous
expression?
C+c+
Which Rh antigen scenario would correctly demonstrate
dosage?
EE 3+ Ee 1+
How does 2+ agglutination appear ?
several small-medium sized agglutinates with a clear
background.
Which of the following statements about Direct Antiglobulin
Testing (DAT) is incorrect?
Direct Antiglobulin Testing tubes must be incubated at 37°C for
10-30 minutes prior to the 3 required washes and the addition of
AHG reagent
If an O Positive recipient has previously never received blood or
blood products,what would be the expected reaction in
the crossmatch tube at the immediate spin (IS) phase with a B
Positive Donor Unit?
Will show agglutination
If an A Positive recipient has previously never received blood or
blood products,what would be the expected reaction in
the crossmatch tube at the immediate spin (IS) phase with an O
Negative Donor Unit?
, Will not show agglutination
If an A Negative recipient has previously never received blood
or blood products,what would be the expected reaction in
the crossmatch tube at the immediate spin (IS) phase with an A
Positive Donor Unit?
Will not show agglutination
Anti-D is Red Blood Cell stimulated and reacts at 37C/AHG (as
it is not IgM and naturally occurring).
If an AB Positive recipient has previously never received blood
or blood products,what would be the expected reaction in
the crossmatch tube at the immediate spin (IS) phase with an A
Negative Donor Unit?
Will not show agglutination
There are two substances that must be antigen typed after an
unexpected antibody is identified in the recipient, and before the
crossmatch can be performed. What are the two substances that
need to be antigen typed?
Donor unit red blood cells and recipient's red blood cells
If an ABO discrepancy was detected or a patient had an
unexpected antibody identified in the past listed in their medical
record, what phases of the crossmatch would need to
be performed?
IS, 37C, and AHG
What is the lectin for Anti-H
Ulex europaeus
Complete Solutions
If all the screen cell lines are negative for the AHG phase and
check cells what phases of the crossmatch procedure need to
performed?
Only IS
When must all phases of the cross match procedure be
performed?
When the screen cell lines have a reaction during the AHG
phase?
When working on solving an antibody panel which of the
following steps would be performed first?
Find the first cell line with a negative reaction at AHG and
perform homozygous cross outs.
How do you determine how many type specific donor units
would need to be antigen typed in order to fun a specific number
of compatible units?
Amount of units needed/Negative antigen incedence
If multiple antibodies: amount of units need/(antibody 1-
negative antigen incidence * antibody 2-negative antigen
incidence)
What would be the proper ABO group selection order for a B
positive recipient?
,B pos, B neg, O pos, O neg
Which Rh antigen pair would be considered heterozygous
expression?
C+c+
Which Rh antigen scenario would correctly demonstrate
dosage?
EE 3+ Ee 1+
How does 2+ agglutination appear ?
several small-medium sized agglutinates with a clear
background.
Which of the following statements about Direct Antiglobulin
Testing (DAT) is incorrect?
Direct Antiglobulin Testing tubes must be incubated at 37°C for
10-30 minutes prior to the 3 required washes and the addition of
AHG reagent
If an O Positive recipient has previously never received blood or
blood products,what would be the expected reaction in
the crossmatch tube at the immediate spin (IS) phase with a B
Positive Donor Unit?
Will show agglutination
If an A Positive recipient has previously never received blood or
blood products,what would be the expected reaction in
the crossmatch tube at the immediate spin (IS) phase with an O
Negative Donor Unit?
, Will not show agglutination
If an A Negative recipient has previously never received blood
or blood products,what would be the expected reaction in
the crossmatch tube at the immediate spin (IS) phase with an A
Positive Donor Unit?
Will not show agglutination
Anti-D is Red Blood Cell stimulated and reacts at 37C/AHG (as
it is not IgM and naturally occurring).
If an AB Positive recipient has previously never received blood
or blood products,what would be the expected reaction in
the crossmatch tube at the immediate spin (IS) phase with an A
Negative Donor Unit?
Will not show agglutination
There are two substances that must be antigen typed after an
unexpected antibody is identified in the recipient, and before the
crossmatch can be performed. What are the two substances that
need to be antigen typed?
Donor unit red blood cells and recipient's red blood cells
If an ABO discrepancy was detected or a patient had an
unexpected antibody identified in the past listed in their medical
record, what phases of the crossmatch would need to
be performed?
IS, 37C, and AHG
What is the lectin for Anti-H
Ulex europaeus
