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C785 Biochemistry & Molecular Biology | 180+ Questions & Answers | PCR, Protein Structure, Metabolism, Enzymes | Western Governors University

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This expert-verified guide features over 180 detailed questions and answers for the C785 – Biochemistry and Molecular Biology course at Western Governors University (WGU). Designed specifically for exam preparation and conceptual mastery, this document covers essential biological and biochemical processes relevant to healthcare, life sciences, and nursing students. Topics span foundational molecular biology—including DNA replication, transcription, and translation—as well as applied content such as: Polymerase Chain Reaction (PCR) steps and requirements Protein folding and levels of structure (primary to quaternary) DNA repair mechanisms including base excision and mismatch repair Enzyme action and inhibition, competitive and noncompetitive Cellular respiration, glycolysis, fermentation, the citric acid cycle, and electron transport chain Metabolic regulation through insulin, glucagon, and metformin Fatty acid metabolism, including beta-oxidation and fatty acid synthesis Physiological insights into hemoglobin, myoglobin, and neurodegenerative conditions like Alzheimer’s All answers are structured for rapid review with clear, concise explanations suitable for test prep, flashcards, or guided study. This resource is ideal for mastering complex biochemical pathways and molecular mechanisms. This document is especially useful for: Pre-nursing, nursing, health science, and biology students Learners in B.S. Health Professions, Pre-medical tracks, and biochemistry-related certification programs Anyone preparing for C785 assessments, TEAS, HESI, or foundational bioscience exams Whether you’re building foundational understanding or reviewing for your final exam, this resource equips you with the critical content and structure needed to succeed. Keywords: C785, biochemistry, molecular biology, PCR, DNA replication, protein structure, transcription, translation, enzymes, metabolism, glycolysis, citric acid cycle, beta oxidation, fatty acids, insulin, glucagon, hemoglobin, Alzheimer’s, WGU

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polymerase chain reaction (PCR) - 🧠ANSWER ✔✔process to copy DNA


What is needed to complete PCR - 🧠ANSWER ✔✔Template DNA,

Nucleotides (dNTPS), DNA Polymerase, and DNA


Steps of PCR - 🧠ANSWER ✔✔denaturation, annealing, elongation


denaturation - 🧠ANSWER ✔✔loss of normal shape of a protein due to heat

or other factor


Annealing - 🧠ANSWER ✔✔cooled to 50c primers stick and want to copy

and add DNA polymerase

,Elongation - 🧠ANSWER ✔✔reaction heated to 70C and DNA polymerase

add nucleotides building a new DNA strand.


base excision repair - 🧠ANSWER ✔✔a modified base is first excised and

then the entire nucleotide is replaced


DNA glycosylase - 🧠ANSWER ✔✔removes damaged DNA


DNA polymerase - 🧠ANSWER ✔✔replaces to damaged DNA ligase seals it


base excision repair - 🧠ANSWER ✔✔removes one single nucleotide


MIsmatch repair - 🧠ANSWER ✔✔The cellular process that uses special

enzymes to fix incorrectly paired nucleotides.


What DNA damage is corrected by mismatch repair - 🧠ANSWER ✔✔errors

in replication fixed

What occurs when DNA polymerase binds to DNA to make RNA -

🧠ANSWER ✔✔transcription ! DNA polymerase takes the individual

nucleotides and matches the them to the parental sequences to ensure a

correct pair. It must bind with RNA primer to work.


nonsense - 🧠ANSWER ✔✔change in 1 nucleotide produces a STOP codon

, silent mutation - 🧠ANSWER ✔✔A mutation that changes a single

nucleotide, but does not change the amino acid created.


Missense - 🧠ANSWER ✔✔a mutation that changes one amino acid


What happens during RNA splicing - 🧠ANSWER ✔✔after transcription, the

introns are removed and the exons are hooks back together


DNA transcription - 🧠ANSWER ✔✔the proccess of copying a section of dna

in order to make proteins


DNA translation - 🧠ANSWER ✔✔Process by which mRNA is converted into

a protein


non-template/coding strand - 🧠ANSWER ✔✔its sequence matches the

sequence of the RNA that is transcribed from the template strand and

codes for a polypeptide


complementary strand - 🧠ANSWER ✔✔A strand of DNA or RNA that has

complementary bases to another strand of DNA or RNA. For instance,

during DNA replication, the new strand that is formed is a complementary

strand. (Complementary bases: A-T, C-G)


DNA template to mRNA is? - 🧠ANSWER ✔✔transcription


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