Portage Microbiology Exam #3 Questions and Answers
Question 1
What is the total magnification of a sample imaged with a 60x objective and a 10x eyepiece? (short answer)
Correct Answer
60 x 10 = 600x magnification
Question 2
True or False: A cell that is adherent, flat, and unstained is easily identified using bright field microscopy
Correct Answer
False. Adherent, flat cells are almost invisible due to the limits on both resolution and contrast
Question 3
Which of the following could be seen clearly by the unaided eye? (multiple choice)
Correct Answer
Protozoa with diameter of 150um and skin cell with diameter of 1500 um (have to be greater than 100um
Page 1 of 7
, Question 4
Label the following unmarked microscope components by matching it with the components provided
Correct Answer
1. Eyepiece
2. Neck
3. Fine adjustment knob
4. Objective
5. Stage
6. Base
Question 5
This type of microscope is best suited for visualizing GFP, RFP and YFP proteins (given list of microscopes)
Correct Answer
Flourescence
Question 6
This type of microscope can provide detailed images of live cells without staining (given list of microscopes)
Correct Answer
Phase-Contrast
Page 2 of 7
Question 1
What is the total magnification of a sample imaged with a 60x objective and a 10x eyepiece? (short answer)
Correct Answer
60 x 10 = 600x magnification
Question 2
True or False: A cell that is adherent, flat, and unstained is easily identified using bright field microscopy
Correct Answer
False. Adherent, flat cells are almost invisible due to the limits on both resolution and contrast
Question 3
Which of the following could be seen clearly by the unaided eye? (multiple choice)
Correct Answer
Protozoa with diameter of 150um and skin cell with diameter of 1500 um (have to be greater than 100um
Page 1 of 7
, Question 4
Label the following unmarked microscope components by matching it with the components provided
Correct Answer
1. Eyepiece
2. Neck
3. Fine adjustment knob
4. Objective
5. Stage
6. Base
Question 5
This type of microscope is best suited for visualizing GFP, RFP and YFP proteins (given list of microscopes)
Correct Answer
Flourescence
Question 6
This type of microscope can provide detailed images of live cells without staining (given list of microscopes)
Correct Answer
Phase-Contrast
Page 2 of 7