NMTCB PET CERTIFICATION REVIEW ACTUAL
EXAM PAPER 2026 QUESTIONS WITH
SOLUTIONS GRADED A+
◉ Actions for Rb82 Generator Alert Level. Answer: Test after every
750 mL has passed through generator
◉ Stop using Rb-82 generator if. Answer: 17L of eleute volume
reached, or 42 days post calibration date, or Sr82 levenis
0.01uCi/mCi of Rb82, or Sr85 level reaches 0.1uCi/mCi of Rb82
◉ Cyclotron produced isotopes and half-life. Answer: C11, 20.3 min;
N13 9.97 min; O15 2.03 min; F18, 109.7 min
◉ Advantages of negative ion cyclotrons. Answer: 1) less nuclear
activation of cyl potion cavity and surroundings, 2) can self-focus
beam of particles, 3) can irradiate 2 targets at once
◉ Isotopes produces by negative ion cyclotrons. Answer: C, N, O =
metabolites
◉ Reaction for chemical labeling of F18-FDG. Answer: Nucleophilic
substitution
,◉ Chemical reaction of FDG. Answer: Electron rich group of atoms
change place on molecule with a positively charged leaving group
◉ Precursor molecule for FDG. Answer: Mannose triflate
◉ Chem formula of FDG. Answer: 2-deoxy-2-[18F]fluoro-D-glucose.
(Fludeoxyglucose F-18)
◉ Intermediate step in FDG synthesis. Answer: Alkaline hydrolysis
of 4 acetyl groups by replacing with OH
◉ Size filter used after purification. Answer: 0.22 um
◉ FDA ref for PET pharma production. Answer: General chapter 823
◉ FDG QA appearance. Answer: Colorless liquid
◉ FDG QA radiochemical identity. Answer: Thin-layer
chromatography Rf value to reference standard
◉ FDG QARadionuclide identity. Answer: Half-life 105 and 115
minutes
,◉ FDG QA bacterial endotoxins test. Answer: LAL (Limulus
amebocyte lysate) <175 USP endotoxins units per max volume
injection; endotoxins< 17.5 EU
◉ FDG QC pH. Answer: Between 4.5 to 7.5
◉ FDG QA Radiochemical purity. Answer: Thin-layered
chromatography radioactive Rf should be not less than 90% of total
radioactivity of chromatography plate
◉ FDG QC Radionuclideic purity. Answer: should be >99.5% of
radionuclidie specified. tested using multi-channel analyzer with
peaks at 0.511 and 1.022 MeV
◉ FDG QC chemical purity. Answer: testing depends upon synthesis
method used to create FDG
◉ Kryptofix 222. Answer: FDG QC chemical purity test - thin-layer
paper chromatography spot test. 50 ug/mL and FDG solution must
demonstrate a less intense response than standard
◉ 2-Chloro-2-deoxy-D-glucose. Answer: FDG QC chemical purity test
used if hydrolysis step was performed with hydrochloric acid. Use
, high-performance liquid chromatography using an electrochemical
detector. Mus not have more than 1 mg CLDG: 2-deoxy-2-D-glucose
◉ FDG QC Residual solvents. Answer: solvents can be in FDG from
cleaning or synthesis solutions. solutions may contain acetonitrle,
ether anddehydrated alcohol. no more 0.04% acetonitrle, and less
than 0.5% of ether or ethanol
◉ FDG QC Sterility. Answer: not performed before dosing due to
need of 14 days to perform. Regulations require final produce to be
placed in soy broth mixture with a thioglycollate growth media for
30 hrs. look for observable bacteria
◉ FDG QC Filter membrane integerity. Answer: final sterility filter
used at end of manufacturing process. Filter must remain intact as
pressure increased to 50 psi (bubble point test)
◉ FDG synthesis room requirements. Answer: room: cGMP, ISO class
5 clean room air hood, 21 CFR 212 for manufacturing of IV drugs
◉ which radiouclides are possible radioactive contaminates in
eluent of Rb82 generators. Answer: Sr82 and Dr85
◉ negative ion PET cyclotrons accelerate which partiles. Answer:
negatively charged hydrogen atoms
EXAM PAPER 2026 QUESTIONS WITH
SOLUTIONS GRADED A+
◉ Actions for Rb82 Generator Alert Level. Answer: Test after every
750 mL has passed through generator
◉ Stop using Rb-82 generator if. Answer: 17L of eleute volume
reached, or 42 days post calibration date, or Sr82 levenis
0.01uCi/mCi of Rb82, or Sr85 level reaches 0.1uCi/mCi of Rb82
◉ Cyclotron produced isotopes and half-life. Answer: C11, 20.3 min;
N13 9.97 min; O15 2.03 min; F18, 109.7 min
◉ Advantages of negative ion cyclotrons. Answer: 1) less nuclear
activation of cyl potion cavity and surroundings, 2) can self-focus
beam of particles, 3) can irradiate 2 targets at once
◉ Isotopes produces by negative ion cyclotrons. Answer: C, N, O =
metabolites
◉ Reaction for chemical labeling of F18-FDG. Answer: Nucleophilic
substitution
,◉ Chemical reaction of FDG. Answer: Electron rich group of atoms
change place on molecule with a positively charged leaving group
◉ Precursor molecule for FDG. Answer: Mannose triflate
◉ Chem formula of FDG. Answer: 2-deoxy-2-[18F]fluoro-D-glucose.
(Fludeoxyglucose F-18)
◉ Intermediate step in FDG synthesis. Answer: Alkaline hydrolysis
of 4 acetyl groups by replacing with OH
◉ Size filter used after purification. Answer: 0.22 um
◉ FDA ref for PET pharma production. Answer: General chapter 823
◉ FDG QA appearance. Answer: Colorless liquid
◉ FDG QA radiochemical identity. Answer: Thin-layer
chromatography Rf value to reference standard
◉ FDG QARadionuclide identity. Answer: Half-life 105 and 115
minutes
,◉ FDG QA bacterial endotoxins test. Answer: LAL (Limulus
amebocyte lysate) <175 USP endotoxins units per max volume
injection; endotoxins< 17.5 EU
◉ FDG QC pH. Answer: Between 4.5 to 7.5
◉ FDG QA Radiochemical purity. Answer: Thin-layered
chromatography radioactive Rf should be not less than 90% of total
radioactivity of chromatography plate
◉ FDG QC Radionuclideic purity. Answer: should be >99.5% of
radionuclidie specified. tested using multi-channel analyzer with
peaks at 0.511 and 1.022 MeV
◉ FDG QC chemical purity. Answer: testing depends upon synthesis
method used to create FDG
◉ Kryptofix 222. Answer: FDG QC chemical purity test - thin-layer
paper chromatography spot test. 50 ug/mL and FDG solution must
demonstrate a less intense response than standard
◉ 2-Chloro-2-deoxy-D-glucose. Answer: FDG QC chemical purity test
used if hydrolysis step was performed with hydrochloric acid. Use
, high-performance liquid chromatography using an electrochemical
detector. Mus not have more than 1 mg CLDG: 2-deoxy-2-D-glucose
◉ FDG QC Residual solvents. Answer: solvents can be in FDG from
cleaning or synthesis solutions. solutions may contain acetonitrle,
ether anddehydrated alcohol. no more 0.04% acetonitrle, and less
than 0.5% of ether or ethanol
◉ FDG QC Sterility. Answer: not performed before dosing due to
need of 14 days to perform. Regulations require final produce to be
placed in soy broth mixture with a thioglycollate growth media for
30 hrs. look for observable bacteria
◉ FDG QC Filter membrane integerity. Answer: final sterility filter
used at end of manufacturing process. Filter must remain intact as
pressure increased to 50 psi (bubble point test)
◉ FDG synthesis room requirements. Answer: room: cGMP, ISO class
5 clean room air hood, 21 CFR 212 for manufacturing of IV drugs
◉ which radiouclides are possible radioactive contaminates in
eluent of Rb82 generators. Answer: Sr82 and Dr85
◉ negative ion PET cyclotrons accelerate which partiles. Answer:
negatively charged hydrogen atoms
