Stem Cells & Genome Therapies 2026
Expert Verified | Ace the Test
Inner cell mass - 🧠ANSWER ✔✔- One of the two first lineages originated
from the fertilised embryo.
- The ICM gives rise to all embryonic tissues and the yolk sac.
Stem cells properties - 🧠ANSWER ✔✔self renewing and potency
Fetal stem cells - 🧠ANSWER ✔✔Cord blood sc, amniotic fluid sc.
Waddington epigenetic landscape - 🧠ANSWER ✔✔Depicts how a cell
progresses from an undifferentiated state to one of a number of discrete,
distinct, differentiated cell fates during development
,James A thomson, Joseph Itskovitz-Eldor, et al. - 🧠ANSWER
✔✔Embryonic stem cell lines derived from humman blastocyst
HESC derivation methods - 🧠ANSWER ✔✔Immunosugical method
Whole/partial embryo culture method
Immunosurgical derivation method (advantages and disadvantages) -
🧠ANSWER ✔✔+
- Easy isolation of ICM from blastocysts possessing large and distinct ICM.
- Selective removal of trophectoderm from expanded blastocysts.
-
- Possibility of contamination with animal pathogens.
- Difficult to isolate ICM from blastocysts having small or indistinct ICM.
Whole embyo derivation method (advantages and disadvantages) -
🧠ANSWER ✔✔+
- Can be used regardless of blastocyst quality.
- No loss of ICM during the process.
- Alleviates contamination with pathogens.
, -
- Risk of trophectoderm overgrowth (impeded growth of ICM).
Partial embyo derivation method (advantages and disadvantages) -
🧠ANSWER ✔✔+
- Efficient for blastocyst with large or small ICM.
- Minimises trophectoderm growth.
- Alleviates contamination with pathogens.
-
- Difficult to isolate portion containing the ICM.
Distinctive pluripotency markers - 🧠ANSWER ✔✔Oct4 and Nanog
HESC culture medium: lab prepared and chemically defined (give pros and
cons for both) - 🧠ANSWER ✔✔Lab prepared:
+ Can adapt media to suit needs.
- Potential batch issues with components.
- Takes time to prepare.
- Daily feeding.
3
COPYRIGHT©JOSHCLAY 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE. PRIVACY
STATEMENT. ALL RIGHTS RESERVED
Expert Verified | Ace the Test
Inner cell mass - 🧠ANSWER ✔✔- One of the two first lineages originated
from the fertilised embryo.
- The ICM gives rise to all embryonic tissues and the yolk sac.
Stem cells properties - 🧠ANSWER ✔✔self renewing and potency
Fetal stem cells - 🧠ANSWER ✔✔Cord blood sc, amniotic fluid sc.
Waddington epigenetic landscape - 🧠ANSWER ✔✔Depicts how a cell
progresses from an undifferentiated state to one of a number of discrete,
distinct, differentiated cell fates during development
,James A thomson, Joseph Itskovitz-Eldor, et al. - 🧠ANSWER
✔✔Embryonic stem cell lines derived from humman blastocyst
HESC derivation methods - 🧠ANSWER ✔✔Immunosugical method
Whole/partial embryo culture method
Immunosurgical derivation method (advantages and disadvantages) -
🧠ANSWER ✔✔+
- Easy isolation of ICM from blastocysts possessing large and distinct ICM.
- Selective removal of trophectoderm from expanded blastocysts.
-
- Possibility of contamination with animal pathogens.
- Difficult to isolate ICM from blastocysts having small or indistinct ICM.
Whole embyo derivation method (advantages and disadvantages) -
🧠ANSWER ✔✔+
- Can be used regardless of blastocyst quality.
- No loss of ICM during the process.
- Alleviates contamination with pathogens.
, -
- Risk of trophectoderm overgrowth (impeded growth of ICM).
Partial embyo derivation method (advantages and disadvantages) -
🧠ANSWER ✔✔+
- Efficient for blastocyst with large or small ICM.
- Minimises trophectoderm growth.
- Alleviates contamination with pathogens.
-
- Difficult to isolate portion containing the ICM.
Distinctive pluripotency markers - 🧠ANSWER ✔✔Oct4 and Nanog
HESC culture medium: lab prepared and chemically defined (give pros and
cons for both) - 🧠ANSWER ✔✔Lab prepared:
+ Can adapt media to suit needs.
- Potential batch issues with components.
- Takes time to prepare.
- Daily feeding.
3
COPYRIGHT©JOSHCLAY 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE. PRIVACY
STATEMENT. ALL RIGHTS RESERVED
