Biochemical Assays for Bacterial Antigen Detection | 2026 Verified Answers & Lab Solutions

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Biochemical Assays For Bacterial Antigen Detection.pdf




RY08 12/17/2024


Biochemical Assays For Bacterial Antigen Detection


Objective
To understand the assays available for detecting bacterial or viral antigens




Notes

ELISA - Enzyme Linked Immunosorbent Assay = utilizes antibodies and colorimetric readout to
indicate an antigen of interest

Color intensity = abundance of antigen
Light Yellow = low amounts of antigen present
Dark yellow = high amounts of antigen present

WESTERN BLOT - Separation of proteins based on size, utilize antibodies and a colorimetric
readout to indicate an antigen of interest

Procedure
Part I: Loading the gel SDS is negative charge dark purple color
Part II: Running the gel
35 mAmps/gel
Part III: Transferring the gel
25 V for - 75 minutes
Part IV: Blocking the gel
2%BSA for ~ 45 minutes at RT room temperature
Place on plate rocker
Part V: Primary antibody
TBST/BSA for ~ 45 minutes at RT
Place on rocker
Part VI: Secondary Antibody
TBST/BSA for ~ 45 minutes at RT
Place on rocker
Part VII: Membrane Development
BCIP/NBT at RT, covered
Place on rocker
Closely Monitor




This study source was downloaded by 1827175 from cliffsnotes.com on 01-08-2026 18:43:59 GMT -06:00

Biochemical Assays For Bacterial Antigen Detection.pdf
Biochemical Assays For Bacterial Antigen Detection.pdf
Biochemical Assays For Bacterial Antigen Detection.pdf
https://www.cliffsnotes.com//study-notes/23217563