CHT EXAM PREP ACTUAL EXAM FOR 2025-2026
& & & & & & &
EXAM QUESTIONS WITH 100% CORRECT & & & & &
ANSWERS | VERIFIED | UPDATED (ACTUAL & & & & & &
EXAM) 2025\2026 LATEST VERSION!! & & & &
What do ASHI Standards say about running both the optical and fluorescent standards
& & & & & & & & & & & & &
for flow cytometry?
& &
A. Run each time the instrument is turned on
& & & & & & & & &
B. Run any time maintenance has occurred
& & & & & & &
C. Run any time problems have occurred that could potentially affect instrument
& & & & & & & & & & & &
function &
D. All of the above :
& & & & &
ANSWER : D. All of the above & & & & & &
The initial recipient workup for a kidney transplant includes all of the following
& & & & & & & & & & & & &
EXCEPT?
A. Establishing the cause of the ESRD (End Stage Renal Disease), if possible
& & & & & & & & & & & & &
B. Measuring the GFR (glomerular filtration rate)
& & & & & & &
C. Performing a health examination and blood work
& & & & & & & &
D. Determining if the patient is a U.S. citizen
& & & & & & & & &
Determining the psychological and social aspects of the candidate :
& & & & & & & & &
ANSWER : D. Determining if the patient is a U.S. citizen
& & & & & & & & & &
Once a candidate has been approved for a transplant, what histocompatibility test(s)
& & & & & & & & & & & &
is/are carried out?
& &
A. HLA typing
& & &
B. Screening for antibodies directed to foreign HLA
& & & & & & & &
,C. ABO typing
& & &
D. HLA typing and antibody screening HLA typing, HLA antibody screening, and ABO
& & & & & & & & & & & &
& typing : &
ANSWER : D. HLA typing and antibody screening HLA typing, HLA antibody
& & & & & & & & & & & &
screening, and ABO typing & & &
All of the following are true about living donors EXCEPT?
& & & & & & & & &
A. Must be over 16 years of age
& & & & & & & &
B. Must be at low risk for any complications associated with transplantation
& & & & & & & & & & & &
C. Must be making this decision freely and for altruistic reasons
& & & & & & & & & & &
D. Must not exhibit any behaviors that may increase the risk for disease transmission
& & & & & & & & & & & & & &
E. Must sign an Informed Consent form :
& & & & & & &
ANSWER : A. Must be over 16 years of age & & & & & & & & &
All of the following are true about unacceptable antigens EXCEPT?
& & & & & & & & &
A. Identified by the presence of antibodies in the transplant candidate directed against
& & & & & & & & & & & & &
foreign HLA & &
B. Entered into UNET for a transplant candidate
& & & & & & & &
C. Used to identify unacceptable donors
& & & & & &
D. Cannot be altered once they are initially entered into UNET
& & & & & & & & & & &
E. Their presence can be altered by vaccination or infection :
& & & & & & & & & &
ANSWER : D. Cannot be altered once they are initially entered into UNET
& & & & & & & & & & & &
What group oversees the initial activities related to deceased donors like obtaining
& & & & & & & & & & & &
family consent for donation, overseeing the clinical management of the donor, and
& & & & & & & & & & & &
beginning the organ allocation procedures?
& & & &
A. UNOS
& &
B. Organ Procurement Organization (OPO)
& & & & &
C. Candidate's transplant program
& & & &
,D. HLA laboratory
& & &
E. Local hospital where the donor is located :
& & & & & & & &
ANSWER : B. Organ Procurement Organization (OPO) & & & & & &
The virtual crossmatch between the deceased donor and potential recipients considers
& & & & & & & & & & &
all of the following EXCEPT?
& & & &
A. Unacceptable antigens
& & &
B. CREG groups and epitopes
& & & & &
C. Antibodies directed to DRA
& & & & &
D. Possible false positive antibody reactions in patient's medical history
& & & & & & & & & &
E. Past positive, current negative antibodies :
& & & & & &
ANSWER : C. Antibodies directed to DRA & & & & & &
A discrepancy between the virtual crossmatch and the physical crossmatch may be
& & & & & & & & & & & &
caused by which of the following?
& & & & &
A. Technical problem when performing physical crossmatch
& & & & & & &
B. Presence of IgM in serum
& & & & & &
C. Denatured antigen on single antigen beads used for antibody test
& & & & & & & & & & &
D. Mismatched allele not on panel used for antibody test
& & & & & & & & & &
E. All of the above :
& & & & &
ANSWER : E. All of the above & & & & & &
Which of the following are required by ASHI's standards for transplant support?
& & & & & & & & & & &
A. Protocols for patients at high risk for allograft rejection
& & & & & & & & & &
B. Policy on requirements for the sensitivity and specificity of the test system required
& & & & & & & & & & & & & &
to support clinical transplant protocols
& & & & &
C. Policy to periodically screen serum samples from each transplant patient for antibody
& & & & & & & & & & & &
& to HLA antigens, including the frequency of screening serum samples
& & & & & & & & & &
D. Policy for storage and maintenance of relevant transplant samples
& & & & & & & & & &
, E. All of these are required by ASHI standards. :
& & & & & & & & &
ANSWER : E. All of these are required by ASHI standards.
& & & & & & & & & &
Preformed HLA antibodies in the transplant candidate that are directed against
& & & & & & & & & & &
mismatched HLA in a potential donor can cause?
& & & & & & &
A. Hyperacute rejection
& & &
B. T cell mediated rejection
& & & & &
C. A delay in engraftment of the kidney
& & & & & & & &
D. Chronic rejection
& & &
E. All of the above :
& & & & &
ANSWER : A. Hyperacute rejection & & & &
Where in the cell does transcription (transferring information from DNA to mRNA)
& & & & & & & & & & & &
take place?
&
A. Nucleus
& &
B. Cytoplasm
& &
C. Endoplasmic reticulum
& & &
D. Golgi apparatus
& & &
E. Lysosome :
& &
ANSWER : A. Nucleus & & &
Which of the following is NOT true about the genetic code?
& & & & & & & & & &
A. Each codon is 3 letters long
& & & & & & &
B. There are codons that "tell" the ribosome to terminate synthesis
& & & & & & & & & & &
C. Each amino acid is specified by only a single codon
& & & & & & & & & & &
D. A codon in the mRNA might be GUA, corresponding to GTA in the DNA
& & & & & & & & & & & & & & &
E. Ribosomes read the code during protein synthesis :
& & & & & & & &
ANSWER : C. Each amino acid is specified by only a single codon
& & & & & & & & & & & &
& & & & & & &
EXAM QUESTIONS WITH 100% CORRECT & & & & &
ANSWERS | VERIFIED | UPDATED (ACTUAL & & & & & &
EXAM) 2025\2026 LATEST VERSION!! & & & &
What do ASHI Standards say about running both the optical and fluorescent standards
& & & & & & & & & & & & &
for flow cytometry?
& &
A. Run each time the instrument is turned on
& & & & & & & & &
B. Run any time maintenance has occurred
& & & & & & &
C. Run any time problems have occurred that could potentially affect instrument
& & & & & & & & & & & &
function &
D. All of the above :
& & & & &
ANSWER : D. All of the above & & & & & &
The initial recipient workup for a kidney transplant includes all of the following
& & & & & & & & & & & & &
EXCEPT?
A. Establishing the cause of the ESRD (End Stage Renal Disease), if possible
& & & & & & & & & & & & &
B. Measuring the GFR (glomerular filtration rate)
& & & & & & &
C. Performing a health examination and blood work
& & & & & & & &
D. Determining if the patient is a U.S. citizen
& & & & & & & & &
Determining the psychological and social aspects of the candidate :
& & & & & & & & &
ANSWER : D. Determining if the patient is a U.S. citizen
& & & & & & & & & &
Once a candidate has been approved for a transplant, what histocompatibility test(s)
& & & & & & & & & & & &
is/are carried out?
& &
A. HLA typing
& & &
B. Screening for antibodies directed to foreign HLA
& & & & & & & &
,C. ABO typing
& & &
D. HLA typing and antibody screening HLA typing, HLA antibody screening, and ABO
& & & & & & & & & & & &
& typing : &
ANSWER : D. HLA typing and antibody screening HLA typing, HLA antibody
& & & & & & & & & & & &
screening, and ABO typing & & &
All of the following are true about living donors EXCEPT?
& & & & & & & & &
A. Must be over 16 years of age
& & & & & & & &
B. Must be at low risk for any complications associated with transplantation
& & & & & & & & & & & &
C. Must be making this decision freely and for altruistic reasons
& & & & & & & & & & &
D. Must not exhibit any behaviors that may increase the risk for disease transmission
& & & & & & & & & & & & & &
E. Must sign an Informed Consent form :
& & & & & & &
ANSWER : A. Must be over 16 years of age & & & & & & & & &
All of the following are true about unacceptable antigens EXCEPT?
& & & & & & & & &
A. Identified by the presence of antibodies in the transplant candidate directed against
& & & & & & & & & & & & &
foreign HLA & &
B. Entered into UNET for a transplant candidate
& & & & & & & &
C. Used to identify unacceptable donors
& & & & & &
D. Cannot be altered once they are initially entered into UNET
& & & & & & & & & & &
E. Their presence can be altered by vaccination or infection :
& & & & & & & & & &
ANSWER : D. Cannot be altered once they are initially entered into UNET
& & & & & & & & & & & &
What group oversees the initial activities related to deceased donors like obtaining
& & & & & & & & & & & &
family consent for donation, overseeing the clinical management of the donor, and
& & & & & & & & & & & &
beginning the organ allocation procedures?
& & & &
A. UNOS
& &
B. Organ Procurement Organization (OPO)
& & & & &
C. Candidate's transplant program
& & & &
,D. HLA laboratory
& & &
E. Local hospital where the donor is located :
& & & & & & & &
ANSWER : B. Organ Procurement Organization (OPO) & & & & & &
The virtual crossmatch between the deceased donor and potential recipients considers
& & & & & & & & & & &
all of the following EXCEPT?
& & & &
A. Unacceptable antigens
& & &
B. CREG groups and epitopes
& & & & &
C. Antibodies directed to DRA
& & & & &
D. Possible false positive antibody reactions in patient's medical history
& & & & & & & & & &
E. Past positive, current negative antibodies :
& & & & & &
ANSWER : C. Antibodies directed to DRA & & & & & &
A discrepancy between the virtual crossmatch and the physical crossmatch may be
& & & & & & & & & & & &
caused by which of the following?
& & & & &
A. Technical problem when performing physical crossmatch
& & & & & & &
B. Presence of IgM in serum
& & & & & &
C. Denatured antigen on single antigen beads used for antibody test
& & & & & & & & & & &
D. Mismatched allele not on panel used for antibody test
& & & & & & & & & &
E. All of the above :
& & & & &
ANSWER : E. All of the above & & & & & &
Which of the following are required by ASHI's standards for transplant support?
& & & & & & & & & & &
A. Protocols for patients at high risk for allograft rejection
& & & & & & & & & &
B. Policy on requirements for the sensitivity and specificity of the test system required
& & & & & & & & & & & & & &
to support clinical transplant protocols
& & & & &
C. Policy to periodically screen serum samples from each transplant patient for antibody
& & & & & & & & & & & &
& to HLA antigens, including the frequency of screening serum samples
& & & & & & & & & &
D. Policy for storage and maintenance of relevant transplant samples
& & & & & & & & & &
, E. All of these are required by ASHI standards. :
& & & & & & & & &
ANSWER : E. All of these are required by ASHI standards.
& & & & & & & & & &
Preformed HLA antibodies in the transplant candidate that are directed against
& & & & & & & & & & &
mismatched HLA in a potential donor can cause?
& & & & & & &
A. Hyperacute rejection
& & &
B. T cell mediated rejection
& & & & &
C. A delay in engraftment of the kidney
& & & & & & & &
D. Chronic rejection
& & &
E. All of the above :
& & & & &
ANSWER : A. Hyperacute rejection & & & &
Where in the cell does transcription (transferring information from DNA to mRNA)
& & & & & & & & & & & &
take place?
&
A. Nucleus
& &
B. Cytoplasm
& &
C. Endoplasmic reticulum
& & &
D. Golgi apparatus
& & &
E. Lysosome :
& &
ANSWER : A. Nucleus & & &
Which of the following is NOT true about the genetic code?
& & & & & & & & & &
A. Each codon is 3 letters long
& & & & & & &
B. There are codons that "tell" the ribosome to terminate synthesis
& & & & & & & & & & &
C. Each amino acid is specified by only a single codon
& & & & & & & & & & &
D. A codon in the mRNA might be GUA, corresponding to GTA in the DNA
& & & & & & & & & & & & & & &
E. Ribosomes read the code during protein synthesis :
& & & & & & & &
ANSWER : C. Each amino acid is specified by only a single codon
& & & & & & & & & & & &
