A short sequence of amino acids at the N-terminus of a protein, that helps target that protein to
a specific location within a cell during its synthesis, is referred to as?
a signal sequence
A protein Z has 3 amino-ends and 3 carboxyl-ends with a total of 300 amino acids residues.
Which of the following might you suggest with some certainty about protein Z?
it has quaternary structure
Which of the following may likely change the shape of a protein leading to its denaturation?
A. an increase in cellular temperature ANS: A
B. an increase in cellular temperature
C. a chemical inhibitor that binds to the protein
D. a change in the presence of charged ions locally
E. choose this choice if all of these could change the shape and may denature a protein
Enzymes would be expected to show an optimal rate of enzyme activity versus which of the
following conditions?
A. pH
B. temperature
C. enzyme concentration
D. only a and b are correct
E. a, b, and c are correct
only a and b are correct
Which of the following is correct concerning a plot of the rate of an enzyme reactionagainst the
concentration of the enzyme's substrate?
, such a plot shows saturation and a maximum rate for the enzyme reaction
Increasing the substrate concentration in an enzymatic reaction could overcome which of the
following?
competitive inhibition of the enzyme
There are 7 known classes of enzymes that catalyze biochemical reactions in cells. The enzyme
class that breaks covalent bonds within polymeric molecules such as glycogen, proteins, and the
nucleic acids by adding water to that bond is which of the following class of enzymes?
hydrolases
Select the best explanation of the effect of a single mutation that results in single amino acid
substitutions within the sequence of amino acids in an enzyme?
it may affect the physicochemical properties of the enzyme such as its optimal temperature
and pH
Some of the drugs used to treat HIV patients are competitive inhibitors of the HIV reverse
transcriptase enzyme.Unfortunately, the high mutation rate of HIV genome means that the virus
rapidly acquires new mutations with amino acid changes that make them resistant to these
competitive inhibitors. Where in the reverse transcriptase enzyme might such amino acid
changes be most likely to occur in this enzyme?
in or near the active site
When one has a severe fever (+104oF), what serious consequence are more likely to occur, if
the fever is not controlled?
a change in the tertiary structure of some critical enzymes
Which of the following is correct about Gram staining of bacterial cell walls?
the peptidoglycan complexes in Gram+ bacteria retains the crystal violet stain
Enzyme A has a measured Km of 1.5 mg and enzyme B has a measured Km of 4.5 mg.Which
enzyme, A or B, would have a better binding affinity for its own substrate?