MOLECULAR DIAGNOSTICS 3RD EDITION
BUCKINGHAM TEST BANK TEST PAPER
2026 EXAM REVIEW WITH COMPLETE
SOLVED ANSWERS
⩥ What is the purpose of a sensitivity control? Answer: shows lower
limit of detection
⩥ true negative Answer: Indicates accurately that a disease is not
present.
⩥ homologous extrinsic Answer: target-derived control with a non-target
derived sequence insert, uses same primers, is not a control for dna
extraction
⩥ heterologous extrinsic Answer: non-target derived controls that are
added to each sample prior to dna extraction, controls for extraction and
amplification but needs a second set of primers
⩥ heterologous intrinsic Answer: non target sequences present in the
sample, requires multiplex or two PCRs, controls for amp and
extraction, needs to be optimized so that control amp does not interfere
with sample amp
, ⩥ Validation Answer: a series of analyte tests using a particular method
to establish method characteristics, must be used to test controls,
specificity, sensitivity, and reproducibility of the assay
⩥ Proficiency testing Answer: Sample tests performed in a clinical
laboratory to determine with what degree of accuracy tests are being
performed. Testing samples are checked in the same manner as patient
specimens.
⩥ Competency Testing Answer: This is mandated by CLIA and tests
individual technologists
⩥ LDT Answer: Laboratory developed test. A subset of in vitro
diagnostic devices are designed, manufactured and offered for clinical
use by a single laboratory
⩥ Bacteria involved in respiratory infection? Answer: Bordetella,
Legionella, Mycobacteria, Chlamydia, Streptococcus, M. tuberculosis
⩥ M. tuberculosis used to be diagnosed by smears but this method
proved to not be accurate. With molecular testing accuracy has been
improved, which tests are used to diagnose M. tuberculosis now?
Answer: PCR and qPCR
BUCKINGHAM TEST BANK TEST PAPER
2026 EXAM REVIEW WITH COMPLETE
SOLVED ANSWERS
⩥ What is the purpose of a sensitivity control? Answer: shows lower
limit of detection
⩥ true negative Answer: Indicates accurately that a disease is not
present.
⩥ homologous extrinsic Answer: target-derived control with a non-target
derived sequence insert, uses same primers, is not a control for dna
extraction
⩥ heterologous extrinsic Answer: non-target derived controls that are
added to each sample prior to dna extraction, controls for extraction and
amplification but needs a second set of primers
⩥ heterologous intrinsic Answer: non target sequences present in the
sample, requires multiplex or two PCRs, controls for amp and
extraction, needs to be optimized so that control amp does not interfere
with sample amp
, ⩥ Validation Answer: a series of analyte tests using a particular method
to establish method characteristics, must be used to test controls,
specificity, sensitivity, and reproducibility of the assay
⩥ Proficiency testing Answer: Sample tests performed in a clinical
laboratory to determine with what degree of accuracy tests are being
performed. Testing samples are checked in the same manner as patient
specimens.
⩥ Competency Testing Answer: This is mandated by CLIA and tests
individual technologists
⩥ LDT Answer: Laboratory developed test. A subset of in vitro
diagnostic devices are designed, manufactured and offered for clinical
use by a single laboratory
⩥ Bacteria involved in respiratory infection? Answer: Bordetella,
Legionella, Mycobacteria, Chlamydia, Streptococcus, M. tuberculosis
⩥ M. tuberculosis used to be diagnosed by smears but this method
proved to not be accurate. With molecular testing accuracy has been
improved, which tests are used to diagnose M. tuberculosis now?
Answer: PCR and qPCR
