Micro lab final lsue ACTUAL QUESTIONS
AND CORRECT ANSWERS
Aseptic Technique- - CORRECT ANSWERS insures that no
contaminating organisms are introduced into culture materials when the latter
are inoculated or handled in some manner.
-aseptic - CORRECT ANSWERS Insures that organisms that are being
handled don't contaminate the handlers or others who may be present.
-aseptic tech - CORRECT ANSWERS Ensuring that only the desired
organism is transferred in each inoculation is of paramount importance in the
identification process.
Work Area Disinfection- - CORRECT ANSWERS work area is first
treated with a disinfectant to kill any microorganisms that may be present. This
process destroys vegetative cells and viruses but may not destroy endospores.
Broth Tube- - CORRECT ANSWERS the tube is inserted into the tube
and twisted several times to ensure that the organisms on the loop are delivered
to the liquid.
Agar Slant- - CORRECT ANSWERS the surface of the slant is
inoculated by drawing the loop up the surface of the slant from the bottom of
the slant to its top.
Stab culture- - CORRECT ANSWERS needle is inserted into the agar
medium by stabbing it into the agar.
Robert Koch- - CORRECT ANSWERS father of the medical
microbiology, was one of the first to recognize that ud he was going to prove
, that a particular bacterium causes a specific disease, it would be necessary to
isolate the agent from all other bacteria and characterize the pathogen.
Pure Culture- - CORRECT ANSWERS Contains only a single kind of an
organism, whereas a mixed culture contains more than one kind. Can study
cultural, morphological, and physiological characteristics of an individual
organism.
Contaminated Culture- - CORRECT ANSWERS contains a desired
organism but also unwanted organisms.
Streak Plate and Pour Plate- - CORRECT ANSWERS both involve
diluting the bacterial cells in a sample to an endpoint where a single cell
divides, giving rise to a single pure colony. The colony is therefore assumed to
be the identical progeny of the original cell and can be picked and used for
further study of the bacterium.
How can bacteria be differentiated? - CORRECT ANSWERS By
characteristics of the colony, such as color, shape, etc.
Isolated Colonies- - CORRECT ANSWERS can then be subculture and
stains prepared to check for purity.
Streak Plate Method- - CORRECT ANSWERS most often used by
microbiologist to obtain pure cultures, simple and allows for economy of
materials. Good spacing between colonies on the plate is critical so that a single
pure colony can be aseptically isolated from quadrant 4 and is used for further
testing and study.
Pour Plate Method- - CORRECT ANSWERS easiest method, method of
separating one species of bacteria from another consists of diluting out one
loopful of organisms with three tubes of liquefied nutrient agar in such a manner
AND CORRECT ANSWERS
Aseptic Technique- - CORRECT ANSWERS insures that no
contaminating organisms are introduced into culture materials when the latter
are inoculated or handled in some manner.
-aseptic - CORRECT ANSWERS Insures that organisms that are being
handled don't contaminate the handlers or others who may be present.
-aseptic tech - CORRECT ANSWERS Ensuring that only the desired
organism is transferred in each inoculation is of paramount importance in the
identification process.
Work Area Disinfection- - CORRECT ANSWERS work area is first
treated with a disinfectant to kill any microorganisms that may be present. This
process destroys vegetative cells and viruses but may not destroy endospores.
Broth Tube- - CORRECT ANSWERS the tube is inserted into the tube
and twisted several times to ensure that the organisms on the loop are delivered
to the liquid.
Agar Slant- - CORRECT ANSWERS the surface of the slant is
inoculated by drawing the loop up the surface of the slant from the bottom of
the slant to its top.
Stab culture- - CORRECT ANSWERS needle is inserted into the agar
medium by stabbing it into the agar.
Robert Koch- - CORRECT ANSWERS father of the medical
microbiology, was one of the first to recognize that ud he was going to prove
, that a particular bacterium causes a specific disease, it would be necessary to
isolate the agent from all other bacteria and characterize the pathogen.
Pure Culture- - CORRECT ANSWERS Contains only a single kind of an
organism, whereas a mixed culture contains more than one kind. Can study
cultural, morphological, and physiological characteristics of an individual
organism.
Contaminated Culture- - CORRECT ANSWERS contains a desired
organism but also unwanted organisms.
Streak Plate and Pour Plate- - CORRECT ANSWERS both involve
diluting the bacterial cells in a sample to an endpoint where a single cell
divides, giving rise to a single pure colony. The colony is therefore assumed to
be the identical progeny of the original cell and can be picked and used for
further study of the bacterium.
How can bacteria be differentiated? - CORRECT ANSWERS By
characteristics of the colony, such as color, shape, etc.
Isolated Colonies- - CORRECT ANSWERS can then be subculture and
stains prepared to check for purity.
Streak Plate Method- - CORRECT ANSWERS most often used by
microbiologist to obtain pure cultures, simple and allows for economy of
materials. Good spacing between colonies on the plate is critical so that a single
pure colony can be aseptically isolated from quadrant 4 and is used for further
testing and study.
Pour Plate Method- - CORRECT ANSWERS easiest method, method of
separating one species of bacteria from another consists of diluting out one
loopful of organisms with three tubes of liquefied nutrient agar in such a manner
