ALAT LATEST 2026 EXAM TEST QUESTIONS AND ANSWERS
GRADED A+
✔✔To prevent animals from becoming dehydrated or even dying, what should the tech
always do after cage racks with auto water are moved or changed - ✔✔Reconnect the
water line
✔✔What type of cage washer is best used for cleaning larger pieces of equipment such
as bulk carts and cage racks - ✔✔rack washer
✔✔What is superheated steam - ✔✔steam that is able to be heated above its normal
temp of 212 F because it is maintained at a higher pressure
✔✔What type of microisolation cage is not mechanically ventilated? How is this type of
cage ventilated? - ✔✔static microisolation cages. A filtered lid allows passive air
exchange into and out of the cage
✔✔Why is feed offered in more than one location for group-house animals -
✔✔because dominant animals may hoard feed
✔✔List two special requirements for cat housing - ✔✔raised resting surface and clean
fresh litter pans
✔✔List two species most commonly found in aquatic cages in a lab setting -
✔✔Xenopus laevis (african clawed frog)
zebrafish
✔✔List four types of plastic used to manufacture small animal caging systems -
✔✔polystyrene, polypropylene, polycarbonate, polyethylene, polyphenylsulfone,
polysulfone
✔✔What are five parameters that determine the necessary size of a primary enclosure -
✔✔experimental requirements, animal's body weight, animal's vertical height or length,
animal's exercise requirements, animal's enrichment needs
✔✔Metabolism cages are most commonly used with which of the following types of lab
animals - ✔✔rodents
✔✔what are 2 types of research studies that use biocontainment caging systems for the
animals - ✔✔infectious disease studies, tox studies
✔✔Squeeze cages are used for what species - ✔✔NHP
,✔✔What type of caging is the term "jump box" used to describe - ✔✔transfer cage
✔✔List three reasons animal rooms must be properly ventilated - ✔✔prevent
accumulation of waste gases, maintain temps, maintain humidity, reduce accumulation
of airborne microorganism
✔✔Which of the following waste gases are prevented from accumulation when animal
rooms are properly ventilated - ✔✔ammonia and carbon dioxide
✔✔Negative effects on animals if daily light schedule is changed - ✔✔stress, changes
in animal physiology that could affect the research, decreased breeding efficiency
✔✔list the four most common types/groups of microorganisms found in the AF -
✔✔bacteria, viruses, fungi, protozoa
✔✔Name three processes used to either remove or kill microorganisms -
✔✔disinfection, sanitization, sterilization
✔✔Why must you remove organic matter such as hair, saliva, urine, and feces before
disinfecting a surface - ✔✔Some sanitizing chemicals are inactivated in the presence of
organic matter, or encrusted matter may trap and shield microorganisms from contact
with sanitizers
✔✔What document provides info on how to handle or safely work with a substance -
✔✔the material safety data sheet (MSDS)
✔✔List three methods of sanitization - ✔✔irradiation, dry heat sterilization, exposure to
ethylene oxide or hydrogen peroxide gas, autoclave, chemical sterilants
✔✔kills bacteria - ✔✔bacteriocidal
✔✔inhibits the growth of bacteria - ✔✔bacteriostatic
✔✔inhibits the growth of viruses - ✔✔virostatic
✔✔kills viruses - ✔✔virucidal
✔✔What is the term for a bacteria that has formed a tough outer coat for protection
against harsh environmental conditions - ✔✔A spore
✔✔Five components of food that are known as nutrients - ✔✔proteins, fats, carbs,
vitamins, minerals
, ✔✔What is the difference between an essential nutrient and a nonessential nutrient -
✔✔essential nutrients are those an animal cannot produce within its body and must be
supplied in the diet. Non-essential nutrients are produced through normal body
metabolism
✔✔In AF, what is usually added to animals' drinking water to prevent the growth of
pseudomonas aeruginose - ✔✔acid
✔✔what is the indigestible form of carbohydrate that provides no energy called -
✔✔fiber
✔✔what are the two types of molecules that join together to make up carbs - ✔✔sugar
and starch molecules
✔✔the main function of body fat is - ✔✔to provide energy
✔✔A deficiency can lead to anemia - ✔✔iron
✔✔electrolytes - ✔✔sodium and chloride
✔✔fat soluble vitamins - ✔✔A,D, E, and K
✔✔cannot be synthesized by NHPs or guinea pigs - ✔✔vitamin c
✔✔deficiency can lead to thinning of the bones - ✔✔calcium
✔✔why must water-soluble vitamins be supplied to an animal on a daily basis - ✔✔they
are not easily stored by the body
✔✔where in the body are the fat-soluble vitamins stored - ✔✔liver
✔✔what is the type of tissue that stores and metabolizes fat called - ✔✔adipose tissue
✔✔which animals have multi-compartmentalized stomachs that can digest some fiber -
✔✔ruminants
✔✔what does the term NPO mean - ✔✔nothing by mouth
✔✔two species that are no fasted prior to surgery - ✔✔rodents and rabbits
✔✔what does environmenttal enrichment provide laboratory animals the opportunity to
do - ✔✔have some variety and engage in species-specific behaviors
GRADED A+
✔✔To prevent animals from becoming dehydrated or even dying, what should the tech
always do after cage racks with auto water are moved or changed - ✔✔Reconnect the
water line
✔✔What type of cage washer is best used for cleaning larger pieces of equipment such
as bulk carts and cage racks - ✔✔rack washer
✔✔What is superheated steam - ✔✔steam that is able to be heated above its normal
temp of 212 F because it is maintained at a higher pressure
✔✔What type of microisolation cage is not mechanically ventilated? How is this type of
cage ventilated? - ✔✔static microisolation cages. A filtered lid allows passive air
exchange into and out of the cage
✔✔Why is feed offered in more than one location for group-house animals -
✔✔because dominant animals may hoard feed
✔✔List two special requirements for cat housing - ✔✔raised resting surface and clean
fresh litter pans
✔✔List two species most commonly found in aquatic cages in a lab setting -
✔✔Xenopus laevis (african clawed frog)
zebrafish
✔✔List four types of plastic used to manufacture small animal caging systems -
✔✔polystyrene, polypropylene, polycarbonate, polyethylene, polyphenylsulfone,
polysulfone
✔✔What are five parameters that determine the necessary size of a primary enclosure -
✔✔experimental requirements, animal's body weight, animal's vertical height or length,
animal's exercise requirements, animal's enrichment needs
✔✔Metabolism cages are most commonly used with which of the following types of lab
animals - ✔✔rodents
✔✔what are 2 types of research studies that use biocontainment caging systems for the
animals - ✔✔infectious disease studies, tox studies
✔✔Squeeze cages are used for what species - ✔✔NHP
,✔✔What type of caging is the term "jump box" used to describe - ✔✔transfer cage
✔✔List three reasons animal rooms must be properly ventilated - ✔✔prevent
accumulation of waste gases, maintain temps, maintain humidity, reduce accumulation
of airborne microorganism
✔✔Which of the following waste gases are prevented from accumulation when animal
rooms are properly ventilated - ✔✔ammonia and carbon dioxide
✔✔Negative effects on animals if daily light schedule is changed - ✔✔stress, changes
in animal physiology that could affect the research, decreased breeding efficiency
✔✔list the four most common types/groups of microorganisms found in the AF -
✔✔bacteria, viruses, fungi, protozoa
✔✔Name three processes used to either remove or kill microorganisms -
✔✔disinfection, sanitization, sterilization
✔✔Why must you remove organic matter such as hair, saliva, urine, and feces before
disinfecting a surface - ✔✔Some sanitizing chemicals are inactivated in the presence of
organic matter, or encrusted matter may trap and shield microorganisms from contact
with sanitizers
✔✔What document provides info on how to handle or safely work with a substance -
✔✔the material safety data sheet (MSDS)
✔✔List three methods of sanitization - ✔✔irradiation, dry heat sterilization, exposure to
ethylene oxide or hydrogen peroxide gas, autoclave, chemical sterilants
✔✔kills bacteria - ✔✔bacteriocidal
✔✔inhibits the growth of bacteria - ✔✔bacteriostatic
✔✔inhibits the growth of viruses - ✔✔virostatic
✔✔kills viruses - ✔✔virucidal
✔✔What is the term for a bacteria that has formed a tough outer coat for protection
against harsh environmental conditions - ✔✔A spore
✔✔Five components of food that are known as nutrients - ✔✔proteins, fats, carbs,
vitamins, minerals
, ✔✔What is the difference between an essential nutrient and a nonessential nutrient -
✔✔essential nutrients are those an animal cannot produce within its body and must be
supplied in the diet. Non-essential nutrients are produced through normal body
metabolism
✔✔In AF, what is usually added to animals' drinking water to prevent the growth of
pseudomonas aeruginose - ✔✔acid
✔✔what is the indigestible form of carbohydrate that provides no energy called -
✔✔fiber
✔✔what are the two types of molecules that join together to make up carbs - ✔✔sugar
and starch molecules
✔✔the main function of body fat is - ✔✔to provide energy
✔✔A deficiency can lead to anemia - ✔✔iron
✔✔electrolytes - ✔✔sodium and chloride
✔✔fat soluble vitamins - ✔✔A,D, E, and K
✔✔cannot be synthesized by NHPs or guinea pigs - ✔✔vitamin c
✔✔deficiency can lead to thinning of the bones - ✔✔calcium
✔✔why must water-soluble vitamins be supplied to an animal on a daily basis - ✔✔they
are not easily stored by the body
✔✔where in the body are the fat-soluble vitamins stored - ✔✔liver
✔✔what is the type of tissue that stores and metabolizes fat called - ✔✔adipose tissue
✔✔which animals have multi-compartmentalized stomachs that can digest some fiber -
✔✔ruminants
✔✔what does the term NPO mean - ✔✔nothing by mouth
✔✔two species that are no fasted prior to surgery - ✔✔rodents and rabbits
✔✔what does environmenttal enrichment provide laboratory animals the opportunity to
do - ✔✔have some variety and engage in species-specific behaviors
