Oxford Cambridge and RSA - 2025
Monday 9 June 2025 – Morning
GCSE (9–1) Biology B (Twenty First Century Science)
J257/04 Depth in Biology (Higher Tier)
Time allowed: 1 hour 45 minutes
(Verified Question Paper With Mark
Scheme Combined June 2025)
,INSTRUCTIONS
• Use black ink. You can use an HB pencil, but only for graphs and diagrams.
• Write your answer to each question in the space provided. If you need extra
space use the lined pages at the end of this booklet. The question numbers
must be clearly shown.
• Answer all the questions.
• Where appropriate, your answer should be supported with working.
Marks might be given for using a correct method, even if your answer is
wrong.
INFORMATION
• The total mark for this paper is 90.
• The marks for each question are shown in brackets [ ].
• Quality of extended response will be assessed in questions marked with an
asterisk (*).
• This document has 24 pages.
ADVICE
• Read each question carefully before you start your answer.
, 3
1 The pupil is part of the human eye.
Pupil
Iris
A student investigates the effect of changing the light level on pupil
diameter. The student uses a lamp with adjustable brightness,
measured in lumens.
The maximum brightness of the lamp is 1200 lumens (100%).
Table 1.1 shows the data the student records.
Table 1.1
Light level Light brightness Distance between Pupil diameter
(%) (lumens) lamp and eye (m) (mm)
80 1.5 4
40 480 1.5 6
20 240 1.5 8
(a) Complete Table 1.2 to identify the variables in the investigation.
Put one tick (✓) in each row to show whether the variable is the dependent variable, the
independent variable or neither.
Table 1.2
Dependent Independent Neither
variable variable
Distance between lamp and eye (m)
Light brightness (lumens)
Pupil diameter (mm)
[2]
© OCR Turn over
2025
, 2
(b) Suggest one other variable not included in Table 1.2 that should be controlled.
............................................................................................................................................
..............
.................................................................................................................................. [1]
(c) Calculate the number of lumens equivalent to a light level of 80%.
Use the data in Table 1.1.
Number of lumens equivalent to a light level of 80% = ............................................
lumens [2]
(d) The student measures the pupil diameter by holding a ruler against the person’s eye.
Suggest a safer way to measure the pupil diameter.
...................................................................................................................................................
.......
.................................................................................................................................. [1]
(e) The student predicts that if a light brightness of 1200 lumens is tested, the pupil
diameter will be approximately 2 mm.
Explain why the student is correct.
...................................................................................................................................................
.......
...................................................................................................................................................
.......
...................................................................................................................................................
.......
...................................................................................................................................................
.......
...................................................................................................................................................
.......
.................................................................................................................................. [3]
© OCR
2025
Monday 9 June 2025 – Morning
GCSE (9–1) Biology B (Twenty First Century Science)
J257/04 Depth in Biology (Higher Tier)
Time allowed: 1 hour 45 minutes
(Verified Question Paper With Mark
Scheme Combined June 2025)
,INSTRUCTIONS
• Use black ink. You can use an HB pencil, but only for graphs and diagrams.
• Write your answer to each question in the space provided. If you need extra
space use the lined pages at the end of this booklet. The question numbers
must be clearly shown.
• Answer all the questions.
• Where appropriate, your answer should be supported with working.
Marks might be given for using a correct method, even if your answer is
wrong.
INFORMATION
• The total mark for this paper is 90.
• The marks for each question are shown in brackets [ ].
• Quality of extended response will be assessed in questions marked with an
asterisk (*).
• This document has 24 pages.
ADVICE
• Read each question carefully before you start your answer.
, 3
1 The pupil is part of the human eye.
Pupil
Iris
A student investigates the effect of changing the light level on pupil
diameter. The student uses a lamp with adjustable brightness,
measured in lumens.
The maximum brightness of the lamp is 1200 lumens (100%).
Table 1.1 shows the data the student records.
Table 1.1
Light level Light brightness Distance between Pupil diameter
(%) (lumens) lamp and eye (m) (mm)
80 1.5 4
40 480 1.5 6
20 240 1.5 8
(a) Complete Table 1.2 to identify the variables in the investigation.
Put one tick (✓) in each row to show whether the variable is the dependent variable, the
independent variable or neither.
Table 1.2
Dependent Independent Neither
variable variable
Distance between lamp and eye (m)
Light brightness (lumens)
Pupil diameter (mm)
[2]
© OCR Turn over
2025
, 2
(b) Suggest one other variable not included in Table 1.2 that should be controlled.
............................................................................................................................................
..............
.................................................................................................................................. [1]
(c) Calculate the number of lumens equivalent to a light level of 80%.
Use the data in Table 1.1.
Number of lumens equivalent to a light level of 80% = ............................................
lumens [2]
(d) The student measures the pupil diameter by holding a ruler against the person’s eye.
Suggest a safer way to measure the pupil diameter.
...................................................................................................................................................
.......
.................................................................................................................................. [1]
(e) The student predicts that if a light brightness of 1200 lumens is tested, the pupil
diameter will be approximately 2 mm.
Explain why the student is correct.
...................................................................................................................................................
.......
...................................................................................................................................................
.......
...................................................................................................................................................
.......
...................................................................................................................................................
.......
...................................................................................................................................................
.......
.................................................................................................................................. [3]
© OCR
2025
