BIOL 251 A&P Exam 1; Portage Learning QUESTIONS AND
ANSWERS WITH COMPLETE SOLUTIONS GRADE A+.
What metric unit would be most appropriate for expressing the size of most
viruses?
Nanometer
The __________ of a compound light microscope focuses light from the
light source on the specimen.
condenser
Most bacteria are in the __________ size range.
1-10 micrometer
Which of the following statements is NOT true for both TEM and SEM?
-The specimen must be sectioned before viewing.
-The illuminating source is an electron beam.
-Both can be used to view specimens smaller than 0.2 micrometers.
-Black-and-white images are produced.
-The microscope is focused using electromagnetic lenses.
The specimen must be sectioned before viewing.
Which of the following types of microscopy can be used to specifically
identify pathogens (such as Mycobacterium tuberculosis or rabies virus) in
specimens?
Fluorescence
If you use a compound light microscope, a 2-µm bacterial cell is best seen at
which magnification?
1
,1000x
A student is observing an object that displays the internal detail on a virus
at great magnification. Which type of microscope is used for this image?
Transmission electron microscope (TEM)
Which of the following kinds of microscopy would be most appropriate for
viewing the shape and arrangement of pili or fimbriae on the surface of a
bacterial cell?
Scanning electron microscopy (SEM)
Which type of light microscopy is used to visualize stained specimens?
Brightfield
When using a 10× ocular lens and a 40× objective, the total magnification
achieved is __________.
400
Resolution is very good when using an electron microscope because the
__________ of the electron beam is much shorter than that of visible light.
wavelength
A(n) __________ is equal to 0.000001 m.
micrometer
__________ is the quality of the microscope that allows one to distinguish
between two points that are very close together.
Resolution
2
, Which of the following is the most convenient and appropriate unit for
expressing the size of an average bacterial cell?
Micrometer
The __________ is the part of the microscope that controls the amount of
light entering the condenser.
diaphragm
Immersion oil is used with the 100 × objective lens of the compound light
microscope to __________.
prevent the loss of resolution due to refraction
Place these structures of the compound light microscope in the order that
light passes through them on the way to the observer's eyes: (1) condenser,
(2) ocular lens, (3) light source, (4) specimen, (5) objective lens.
light source → condenser → specimen → objective lens → ocular lens
Which of the following types of microscopy is used to study the structure of
the slimy accumulation of bacteria on an IV catheter tip?
Scanning acoustic
__________ acts to decrease refraction of light rays and thus increase
resolution.
Immersion oil
Which type of microscope uses a metal and diamond probe that is gently
forced down along the surface of a specimen?
Atomic force
3
ANSWERS WITH COMPLETE SOLUTIONS GRADE A+.
What metric unit would be most appropriate for expressing the size of most
viruses?
Nanometer
The __________ of a compound light microscope focuses light from the
light source on the specimen.
condenser
Most bacteria are in the __________ size range.
1-10 micrometer
Which of the following statements is NOT true for both TEM and SEM?
-The specimen must be sectioned before viewing.
-The illuminating source is an electron beam.
-Both can be used to view specimens smaller than 0.2 micrometers.
-Black-and-white images are produced.
-The microscope is focused using electromagnetic lenses.
The specimen must be sectioned before viewing.
Which of the following types of microscopy can be used to specifically
identify pathogens (such as Mycobacterium tuberculosis or rabies virus) in
specimens?
Fluorescence
If you use a compound light microscope, a 2-µm bacterial cell is best seen at
which magnification?
1
,1000x
A student is observing an object that displays the internal detail on a virus
at great magnification. Which type of microscope is used for this image?
Transmission electron microscope (TEM)
Which of the following kinds of microscopy would be most appropriate for
viewing the shape and arrangement of pili or fimbriae on the surface of a
bacterial cell?
Scanning electron microscopy (SEM)
Which type of light microscopy is used to visualize stained specimens?
Brightfield
When using a 10× ocular lens and a 40× objective, the total magnification
achieved is __________.
400
Resolution is very good when using an electron microscope because the
__________ of the electron beam is much shorter than that of visible light.
wavelength
A(n) __________ is equal to 0.000001 m.
micrometer
__________ is the quality of the microscope that allows one to distinguish
between two points that are very close together.
Resolution
2
, Which of the following is the most convenient and appropriate unit for
expressing the size of an average bacterial cell?
Micrometer
The __________ is the part of the microscope that controls the amount of
light entering the condenser.
diaphragm
Immersion oil is used with the 100 × objective lens of the compound light
microscope to __________.
prevent the loss of resolution due to refraction
Place these structures of the compound light microscope in the order that
light passes through them on the way to the observer's eyes: (1) condenser,
(2) ocular lens, (3) light source, (4) specimen, (5) objective lens.
light source → condenser → specimen → objective lens → ocular lens
Which of the following types of microscopy is used to study the structure of
the slimy accumulation of bacteria on an IV catheter tip?
Scanning acoustic
__________ acts to decrease refraction of light rays and thus increase
resolution.
Immersion oil
Which type of microscope uses a metal and diamond probe that is gently
forced down along the surface of a specimen?
Atomic force
3
