Enriched medium
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-Contains complex organic substances
-Supports the growth of fastidious bacteria
Ex: Blood agar, Thayer-Martin
Transmission electron microscope
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, -often used to view structures of cells and viruses
-electrons are transmitted through the specimen
-The specimen must be very thin (sliced), and stained to increase image
contrast
Pasteurazation
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Mild heating used to kill off spoiled bacteria, but not hot enough to
evaporate the liquid
Name the microbes that cause tuberculosis and leprosy. what stain is used to identify
them?
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-mycobacterium tuberculosis and mycobacterium leprae
-Acid-fast stain is used to identify them
how are the major microorganism groups broken down into prokaryotic and
eukaryotic
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Prokaryotes: bacteria and archae
Eukaryotes: fungi, algae, protozoa, and helmynths
, Viruses are in their own group
Lysis
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The physical rupture or deterioration of a cell
Phase-contrast microscopy
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-transforms subtle changes in light waves passing through a specimen into
differences in light intensity
-allows differentiation of internal components of live, unstained cells
-useful for viewing intracellular structures
Subculture
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-a new cell or microbiological culture made by transferring some or all
cells from a previous culture to fresh growth medium
Protoplast
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, a bacterial cell whose cell wall is completely lacking and that is vulnerable
to osmotic lysis
The Five I's
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-Inoculation
-Incubation
-Isolation
-Inspection
-Identification
Immunology
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This branch of Microbiology studies the complex web of protective
substances and cells produced in response to infection.
Assay medium
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-used to test effectiveness of antibiotics, disinfectants, and antiseptics
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-Contains complex organic substances
-Supports the growth of fastidious bacteria
Ex: Blood agar, Thayer-Martin
Transmission electron microscope
Give this one a try later!
, -often used to view structures of cells and viruses
-electrons are transmitted through the specimen
-The specimen must be very thin (sliced), and stained to increase image
contrast
Pasteurazation
Give this one a try later!
Mild heating used to kill off spoiled bacteria, but not hot enough to
evaporate the liquid
Name the microbes that cause tuberculosis and leprosy. what stain is used to identify
them?
Give this one a try later!
-mycobacterium tuberculosis and mycobacterium leprae
-Acid-fast stain is used to identify them
how are the major microorganism groups broken down into prokaryotic and
eukaryotic
Give this one a try later!
Prokaryotes: bacteria and archae
Eukaryotes: fungi, algae, protozoa, and helmynths
, Viruses are in their own group
Lysis
Give this one a try later!
The physical rupture or deterioration of a cell
Phase-contrast microscopy
Give this one a try later!
-transforms subtle changes in light waves passing through a specimen into
differences in light intensity
-allows differentiation of internal components of live, unstained cells
-useful for viewing intracellular structures
Subculture
Give this one a try later!
-a new cell or microbiological culture made by transferring some or all
cells from a previous culture to fresh growth medium
Protoplast
Give this one a try later!
, a bacterial cell whose cell wall is completely lacking and that is vulnerable
to osmotic lysis
The Five I's
Give this one a try later!
-Inoculation
-Incubation
-Isolation
-Inspection
-Identification
Immunology
Give this one a try later!
This branch of Microbiology studies the complex web of protective
substances and cells produced in response to infection.
Assay medium
Give this one a try later!
-used to test effectiveness of antibiotics, disinfectants, and antiseptics
