AAB MT CHEMISTRY EXAM 2025 WITH
COMPLETE SOLUTIONS GRADED A+
Hemoglobin is composed of heme and globin.
Correct Answers: True.
The heme portion of hemoglobin contains iron.
Correct Answers: True.
Iron in hemoglobin is in the ferrous state.
Correct Answers: True.
Each hemoglobin molecule can carry how many oxygen molecules?
Correct Answers: Four.
Oxyhemoglobin is hemoglobin bound to oxygen.
Correct Answers: True.
Deoxyhemoglobin is hemoglobin without oxygen.
Correct Answers: True.
Carboxyhemoglobin is hemoglobin bound to carbon monoxide.
Correct Answers: True.
Methemoglobin has iron in the ferric state.
Correct Answers: True.
Sulfhemoglobin is a permanently altered form from sulfur-containing drugs.
Correct Answers: True.
,A glycated form of hemoglobin measured to monitor long-term diabetes control
is called?
Correct Answers: Hemoglobin A1c.
The oxygen dissociation curve describes the relationship between?
Correct Answers: Partial pressure of oxygen and hemoglobin saturation.
A shift of the oxygen dissociation curve to the right indicates decreased oxygen
affinity.
Correct Answers: True.
What causes a right shift in the oxygen dissociation curve?
Correct Answers: Increased temperature, increased 2,3-DPG, decreased pH
(acidosis).
The Bohr effect describes how increased CO2 and decreased pH decrease oxygen
affinity.
Correct Answers: True.
2,3-Diphosphoglycerate is produced in red cells and decreases oxygen affinity.
Correct Answers: True.
Hematocrit measures the percentage of blood volume occupied by red cells.
Correct Answers: True.
Normal adult hemoglobin types are primarily Hb A, Hb A2, and Hb F.
Correct Answers: True.
What is the primary adult hemoglobin, consisting of two alpha and two beta
chains?
Correct Answers: Hemoglobin A.
Hb F has a higher affinity for oxygen than Hb A.
Correct Answers: True.
,A hemoglobin variant causing sickle cell disease is?
Correct Answers: Hemoglobin S.
In Hemoglobin S, glutamic acid is replaced by valine on the beta chain.
Correct Answers: True.
What is the laboratory test that separates hemoglobin variants based on charge?
Correct Answers: Hemoglobin electrophoresis.
What principle does alkaline electrophoresis for hemoglobin use?
Correct Answers: Separation based on charge at alkaline pH.
Cellulose acetate at pH 8.4-8.6 is a common medium for hemoglobin
electrophoresis.
Correct Answers: True.
Citrate agar electrophoresis at acidic pH helps distinguish between Hb S and Hb C.
Correct Answers: True.
Hb C disease is characterized by target cells and mild hemolytic anemia.
Correct Answers: True.
What is the confirmatory test for sickle cell disease showing tactoid crystals?
Correct Answers: Hemoglobin solubility test (using sodium dithionite).
The Sickledex test is a solubility test.
Correct Answers: True.
What test quantifies Hb A2, important for beta-thalassemia diagnosis?
Correct Answers: Column chromatography or HPLC.
High-Performance Liquid Chromatography is a primary method for hemoglobin
identification and quantification.
Correct Answers: True.
, What does an increased Hb F level in an adult suggest?
Correct Answers: Beta-thalassemia, hereditary persistence of Hb F, or some bone
marrow disorders.
Unstable hemoglobins may precipitate as Heinz bodies.
Correct Answers: True.
The isopropanol precipitation test detects unstable hemoglobins.
Correct Answers: True.
Methemoglobin cannot bind oxygen.
Correct Answers: True.
The body reduces methemoglobin back to hemoglobin using the enzyme?
Correct Answers: Methemoglobin reductase (NADH-cytochrome b5 reductase).
Cyanmethemoglobin method is used to measure hemoglobin concentration.
Correct Answers: True.
What reagent is used in the cyanmethemoglobin method?
Correct Answers: Drabkin's reagent.
Drabkin's reagent contains potassium ferricyanide and potassium cyanide.
Correct Answers: True.
In the cyanmethemoglobin method, all forms of hemoglobin except
sulfhemoglobin are converted.
Correct Answers: True.
The absorbance of cyanmethemoglobin is measured at what wavelength?
Correct Answers: 540 nm.
MCH stands for Mean Corpuscular Hemoglobin.
Correct Answers: True.
COMPLETE SOLUTIONS GRADED A+
Hemoglobin is composed of heme and globin.
Correct Answers: True.
The heme portion of hemoglobin contains iron.
Correct Answers: True.
Iron in hemoglobin is in the ferrous state.
Correct Answers: True.
Each hemoglobin molecule can carry how many oxygen molecules?
Correct Answers: Four.
Oxyhemoglobin is hemoglobin bound to oxygen.
Correct Answers: True.
Deoxyhemoglobin is hemoglobin without oxygen.
Correct Answers: True.
Carboxyhemoglobin is hemoglobin bound to carbon monoxide.
Correct Answers: True.
Methemoglobin has iron in the ferric state.
Correct Answers: True.
Sulfhemoglobin is a permanently altered form from sulfur-containing drugs.
Correct Answers: True.
,A glycated form of hemoglobin measured to monitor long-term diabetes control
is called?
Correct Answers: Hemoglobin A1c.
The oxygen dissociation curve describes the relationship between?
Correct Answers: Partial pressure of oxygen and hemoglobin saturation.
A shift of the oxygen dissociation curve to the right indicates decreased oxygen
affinity.
Correct Answers: True.
What causes a right shift in the oxygen dissociation curve?
Correct Answers: Increased temperature, increased 2,3-DPG, decreased pH
(acidosis).
The Bohr effect describes how increased CO2 and decreased pH decrease oxygen
affinity.
Correct Answers: True.
2,3-Diphosphoglycerate is produced in red cells and decreases oxygen affinity.
Correct Answers: True.
Hematocrit measures the percentage of blood volume occupied by red cells.
Correct Answers: True.
Normal adult hemoglobin types are primarily Hb A, Hb A2, and Hb F.
Correct Answers: True.
What is the primary adult hemoglobin, consisting of two alpha and two beta
chains?
Correct Answers: Hemoglobin A.
Hb F has a higher affinity for oxygen than Hb A.
Correct Answers: True.
,A hemoglobin variant causing sickle cell disease is?
Correct Answers: Hemoglobin S.
In Hemoglobin S, glutamic acid is replaced by valine on the beta chain.
Correct Answers: True.
What is the laboratory test that separates hemoglobin variants based on charge?
Correct Answers: Hemoglobin electrophoresis.
What principle does alkaline electrophoresis for hemoglobin use?
Correct Answers: Separation based on charge at alkaline pH.
Cellulose acetate at pH 8.4-8.6 is a common medium for hemoglobin
electrophoresis.
Correct Answers: True.
Citrate agar electrophoresis at acidic pH helps distinguish between Hb S and Hb C.
Correct Answers: True.
Hb C disease is characterized by target cells and mild hemolytic anemia.
Correct Answers: True.
What is the confirmatory test for sickle cell disease showing tactoid crystals?
Correct Answers: Hemoglobin solubility test (using sodium dithionite).
The Sickledex test is a solubility test.
Correct Answers: True.
What test quantifies Hb A2, important for beta-thalassemia diagnosis?
Correct Answers: Column chromatography or HPLC.
High-Performance Liquid Chromatography is a primary method for hemoglobin
identification and quantification.
Correct Answers: True.
, What does an increased Hb F level in an adult suggest?
Correct Answers: Beta-thalassemia, hereditary persistence of Hb F, or some bone
marrow disorders.
Unstable hemoglobins may precipitate as Heinz bodies.
Correct Answers: True.
The isopropanol precipitation test detects unstable hemoglobins.
Correct Answers: True.
Methemoglobin cannot bind oxygen.
Correct Answers: True.
The body reduces methemoglobin back to hemoglobin using the enzyme?
Correct Answers: Methemoglobin reductase (NADH-cytochrome b5 reductase).
Cyanmethemoglobin method is used to measure hemoglobin concentration.
Correct Answers: True.
What reagent is used in the cyanmethemoglobin method?
Correct Answers: Drabkin's reagent.
Drabkin's reagent contains potassium ferricyanide and potassium cyanide.
Correct Answers: True.
In the cyanmethemoglobin method, all forms of hemoglobin except
sulfhemoglobin are converted.
Correct Answers: True.
The absorbance of cyanmethemoglobin is measured at what wavelength?
Correct Answers: 540 nm.
MCH stands for Mean Corpuscular Hemoglobin.
Correct Answers: True.
