PIL AB - Methods of Humane Killing, Health and Safety Quiz, PIL AB - legislation quiz
Questions with Correct Answers Latest Update 2025/2026
overdose of an anaesthetic - Answers all animals
exposure to carbon dioxide gas in a rising concentration - Answers birds and rodents up to
1.5kg
(NOT neonatal)
dislocation of the neck - Answers rodents and rabbits up to 0.5kg
Cooling of fetuses followed by immersion in cold tissue fixative - Answers fetal mice, rats,
rabbits
decapitation - Answers fetal mammals and birds up to 50g
What official terminology is used in the UK to describe the categories of laboratory biosafety -
Answers Containment Level Biosafety (BSL)
What does LAA stand for? - Answers Laboratory Animal Allergens
Carry out DNA extration with solution considered phenol which could cause injury if it spills.
Risk or hazard? - Answers Risk
Where can allergens be present? - Answers Soiled bedding; urine/faeces; fur/dander
what are symptoms of sensitization/allergy? - Answers rhinitis
conjunctivitis
what does COSHH control? - Answers All substances hazardous to health
I do not need to register with the University Occupational Health Service (UOHS) as I am only
working with a small number of mice during my DPhil. T/F - Answers False
What to register with Occ Health (using HS1 form)? - Answers Before conducting any workings
with lab animals
Minimum PPE should be worn when directly working with Lab Animals? - Answers gloves,
hairnets, scrubs/oversuit/lab coat, face-fitted mask
What does IVC stand for? - Answers individually ventilated cage
Do surgical type masks protect against Lab Animal Allergens - Answers No
Where must you report a Sharps, Splash, or Bite injury? - Answers Manager/supervisor; occ
health; IRIS
, Do provisions of Cruelty Act of 1876 and the Protection of Animals Act 1911 still apply? -
Answers Cruelty of animals act appealed by section 27 of 1986 acts;
Most of 191a act is amended and included in more general Animal Welfare Act 2006.
definition of death according to ASPA - Answers Permanent cesssation of circulation; or
destruction of the brain
Criteria procedure must satisfy under ASPA regulation? - Answers Scientific procedure;
Applied to protected animal;
Have potential to cause pain and suffering, distress, or lasting harm [Section 2(1)] (PSDLH)
What purpose does Oxford require Establishment License (PEL)? - Answers To allow:
-scientific procedures to be carried out here;
-breed animals;
-to supply experimental animals
PEL - Holder of establishment license - Answers Gill Aitken
Other people on University's Establishment License (PEL) - Answers -named compliance officer;
-named vet surgeon;
-holder of establishment license;
-named animal care and welfare officer;
-named information officer;
named compliance officer (NCO) - Answers keeps track of compliance with law, usually same
as PEL holder
named vet surgeon (NVT) - Answers for expert advice on welfare
holder of establishment license - Answers
named animal care and welfare officer (NACWO) - Answers responsible for day to day welfare
named information officer (NIO) - Answers up to date with info on species, 3Rs, etc.
Severity limits of procedures - Answers -mild
-moderate
-severe
Questions with Correct Answers Latest Update 2025/2026
overdose of an anaesthetic - Answers all animals
exposure to carbon dioxide gas in a rising concentration - Answers birds and rodents up to
1.5kg
(NOT neonatal)
dislocation of the neck - Answers rodents and rabbits up to 0.5kg
Cooling of fetuses followed by immersion in cold tissue fixative - Answers fetal mice, rats,
rabbits
decapitation - Answers fetal mammals and birds up to 50g
What official terminology is used in the UK to describe the categories of laboratory biosafety -
Answers Containment Level Biosafety (BSL)
What does LAA stand for? - Answers Laboratory Animal Allergens
Carry out DNA extration with solution considered phenol which could cause injury if it spills.
Risk or hazard? - Answers Risk
Where can allergens be present? - Answers Soiled bedding; urine/faeces; fur/dander
what are symptoms of sensitization/allergy? - Answers rhinitis
conjunctivitis
what does COSHH control? - Answers All substances hazardous to health
I do not need to register with the University Occupational Health Service (UOHS) as I am only
working with a small number of mice during my DPhil. T/F - Answers False
What to register with Occ Health (using HS1 form)? - Answers Before conducting any workings
with lab animals
Minimum PPE should be worn when directly working with Lab Animals? - Answers gloves,
hairnets, scrubs/oversuit/lab coat, face-fitted mask
What does IVC stand for? - Answers individually ventilated cage
Do surgical type masks protect against Lab Animal Allergens - Answers No
Where must you report a Sharps, Splash, or Bite injury? - Answers Manager/supervisor; occ
health; IRIS
, Do provisions of Cruelty Act of 1876 and the Protection of Animals Act 1911 still apply? -
Answers Cruelty of animals act appealed by section 27 of 1986 acts;
Most of 191a act is amended and included in more general Animal Welfare Act 2006.
definition of death according to ASPA - Answers Permanent cesssation of circulation; or
destruction of the brain
Criteria procedure must satisfy under ASPA regulation? - Answers Scientific procedure;
Applied to protected animal;
Have potential to cause pain and suffering, distress, or lasting harm [Section 2(1)] (PSDLH)
What purpose does Oxford require Establishment License (PEL)? - Answers To allow:
-scientific procedures to be carried out here;
-breed animals;
-to supply experimental animals
PEL - Holder of establishment license - Answers Gill Aitken
Other people on University's Establishment License (PEL) - Answers -named compliance officer;
-named vet surgeon;
-holder of establishment license;
-named animal care and welfare officer;
-named information officer;
named compliance officer (NCO) - Answers keeps track of compliance with law, usually same
as PEL holder
named vet surgeon (NVT) - Answers for expert advice on welfare
holder of establishment license - Answers
named animal care and welfare officer (NACWO) - Answers responsible for day to day welfare
named information officer (NIO) - Answers up to date with info on species, 3Rs, etc.
Severity limits of procedures - Answers -mild
-moderate
-severe
