Lab Exam #1 – Microbiology Examples Questions And Answers Solved.

Contamination is most often due to ... - correct answers failure to adhere to appropriate procedures Differentiation of bacteria into two major groups using the Gram stain is based on ... - correct answers differences in cell wall structure A culture media that contains one or more ingredients that inhibit the growth of certain microbes, but not others, is called a(n) ... - correct answers selective media Microscope slides and any broken glass generated in the laboratory should be ... - correct answers placed into the cardboard glass disposal box located in the laboratory area Which is the greater distance - 1 mile or 1 kilometer? - correct answers1 mile Which of the following is a function accomplished by heat fixation? - correct answers enhances absorption of the primary stain If the instructions state to examine a specimen at a total magnification of 400X, and your microscope has a 10X ocular lens; which objective lens must be used? - correct answers40X Due to the nature of the microbiology laboratory environment, which of the following is a primary concern? - correct answers safety What is the purpose of a "blue" filter used with a microscope? - correct answers restrict longer wavelengths of light from striking the specimen The mordant used for the Gram stain procedure is ... - correct answers iodine The total magnification achieved when using a 15X ocular lens and 45X objective lens would be ... - correct answers675X What is the base unit for linear distance in the metric system? - correct answersmeter How many kilograms is equal to 5000 milligrams? - correct answers0.005 kilograms If John is 6'2" tall and the doorway is 2 meters tall, can John walk through the doorway upright or must he bend over to avoid hitting his head? - correct answersJohn can walk through the doorway upright without hitting his head How many centimeters is equal to 10 inches? - correct answers25.4 centimeters Which is the greater volume - 5 quarts or 6 liters? - correct answers6 liters The primary stain used for the Gram stain procedure is ... - correct answerscrystal violet The magnification achieved by a light microscope is ... - correct answersa function of the interaction of light rays with the curvature of the lens Who is credited with the invention of the first usable microscope? - correct answersAntoine van Leeuwenhoek How close together can two objects be and be seen separately if your microscope is using visible light with wavelength equal to 750nm and an objective lens with a numerical aperture equal to 1.25? - correct answers0.3 micrometers The inhibitor used in Mannitol Salt Agar is ... - correct answers7.5% NaCl Disease-causing microbes are called ... - correct answerspathogens Which of the following types of culture media suppresses the growth of most bacteria, but allows fungi to grow well? - correct answersSabouraud dextrose agar The bending or changing the angle of the light rays as it passes through a medium such as a lens is called ... - correct answersrefraction Tryptic Soy Broth is a ... - correct answersgeneral purpose liquid culture media Which of the following bacteria and culture media combinations is likely to yield a green metallic sheen appearance? - correct answers Escherichia coli growing on Eosin Methylene Blue Agar One kilogram is equal to ... - correct answers1,000 grams When performing the Gram stain procedure, which of the following steps must occur before the other three? - correct answers heat fixation A microscope that utilizes visible light and more than one lens is called a(n) ... - correct answers compound microscope How many liters is equal to 5 gallons? - correct answers18.92 liters Which of the following is NOT a critical factor when performing culture procedures in the microbiology laboratory? - correct answers eat a healthy breakfast Bacteria stained using the Gram stain procedure that are observed to be spherical and purple would be described as ... - correct answers gram postive cocci A chemical that enhances absorption and helps solidify the relationship between the primary stain and the material being stained is called a(n) ... - correct answers mordant What total magnification is required in order to visualize individual bacteria? - correct answers Which of the following statements regarding selective and/or differential media is correct? - correct answers Lactose fermenting bacteria appear pink on MacConkey Agar Which is heavier - 2 kilograms or 250 grams? - correct answers2 kilograms The indicator used in Mannitol Salt Agar is ... - correct answers phenol red The differential characteristic evaluated by MacConkey agar is ... - correct answers lactose fermentation If the distance from Dallas, Texas to Atlanta, Goegia is 925 miles, how many kilometers would it be from Atlanta, Georgia to Dallas, Texas? - correct answers1492 kilometers (1488.64) A major consideration (i.e. enemy) in the microbiology laboratory is ... - correct answers contamination Which of the following states the sequence of events for the Gram stain in proper sequence? - correct answers crystal violet - iodine - decolorizer - safranin Gram negative bacteria generally appear __________ as a result of the gram stain procedure/ - correct answers pink Which of the following prefixes is used in the metric system to mean "ten times the base unit"? - correct answers deca- Which of the following statements is accurate regarding use of the microscope? - correct answers you should always begin the examination at low power magnification Bacteria observed microscopically after being stained using the Gram stain that are described as Gram negative bacilli would be ... - correct answers pink and elongated, rod-shaped What is the metric system base unit for weight? - correct answers gram The flat, disk-like part of the microscope that is used to adjust the amount of light transmitted to the object to be examined is called the ... - correct answers iris diaphragm Which of the following is a product of red algae that is used as a solidifying agent in many artificial culture medias? - correct answers agar What is the purpose of the "4-quadrant method" of inoculating culture media in plate form? - correct answers obtain isolated colonies of the organism The counterstain used for the Gram stain procedure is ... - correct answer safranin