Microbiology LAB Exam Miller Review Questions And Answers Guaranteed Success.

Total Magnification - correct answer stake the power of the objective (4X, 10X, 40x) and multiply by the power of the eyepiece, usually 10X. 4x - correct answers Scanning lens 10x - correct answers low power lens 40x - correct answers high power dry lens 100x - correct answers High power oil immersion lens. Oil must be used to focus properly. Condenser - correct answers used to stage clamp. adjust both intensity and contrast of light. Aperture lever located on front adjusts contrast Ocular - correct answers moves left/right to adjust interpupillary distance- use scale located between eyepieces NOTE: left eyepiece rotates in/out to compensate for vision irregularities. Normally set to 0 on scale. 10x magnification. Binocular Tube - correct answers swivels to permit eyepiece housing to rotate. Slightly loosen screw to rotate. Lock screw securely. Stage Adjustment knobs - correct answers Knobs move stage clamp left/right, north/south. Position specimen centered on light source. Specimen is clipped into place by CONDENSER- used to stage clamp. Lamp Intensity Dial - correct answers used to adjust light intensity. Normally set between 8-10. Arm - correct answersthis part on the side of the microscope is used to support it when it is carried Base - correct answerssupports microscope and is used to carry instrument Coarse Focus Knob - correct answersmoves stage height in large increments. Used only for finding and initially focusing on specimen using low power lenses only. Do not use with 40x and 100x lenses! Fine Adjustment knob - correct answersmoves stage height in fine increments. Used with low power lenses for final focus. Must be used when using 40x & 100x lenses Explain how to clean and care for microscope - correct answers Explain how to focus the microscope - correct answers Hemolysis of Sheep's Blood Agar - Alpha hemolysis of S. oralis - correct answers Hemolysis of Sheep's Blood Agar - beta-hemolysis of S. pyogenes - correct answers Hemolysis of Sheep's Blood Agar - Gamma-hemolysis of E. coli - correct answers Gelatin Agar - Gelatinase positive results of B. subtitles - correct answers Gelatin Agar - Gelatinase negative results of E. coli - correct answers Starch Agar - Amylase positive results of B. subtils - correct answers Starch Agar - Amylase negative results of E. coli - correct answers IMVICs - Indole: positive red ring and negative yellow ring - correct answers IMVICs - Methyl Red: positive top and negative yellow top - correct answers IMVICs - Vogues Proskauer: positive rust color and negative straw - correct answers IMVICs - Citrate: positive blue and negative green. - correct answers Gram Stain of S. aureus - correct answers Gram Stain of B. megaterium - correct answers Gram Stain of S. pyogenes - correct answers Gram Stain of E. coli - correct answers Mixed Gram stain of S. aureus and E. coli - correct answers Capsule Stain of K. pneumoniae - correct answers Fatty Bodies - correct answers Flagella Stain of an amphitrichous spirillium - correct answers Tetrad formation of M. luteus - correct answers Mixed Acid-Fast Stain of S. aureus and M. smegmatis - correct answers Negative Stain of B. subtilis - correct answers Spore Stain of Bacillus sp. - correct answers