MCB 150 EXAM 4 – Questions With Complete Solutions
It is often desirable to express eukaryotic genes in bacteria,
which can make a protein of interest quickly and cheaply. What
is TRUE about this kind of genetic transformation?
a) The expressed gene and the genes for all of its regulatory
elements must be incorporated into the bacterial chromosome.
b) The expressed gene must be incorporated into the bacterial
chromosome, but its promoter region should be replaced with a
bacterial promoter.
c) Only polycistronic genes are capable of being expressed in a
bacteria.
d) The expressed gene and the gene for RNA Polymerase II
must both be incorporated into the bacterial genome.
e) Only the transcribed region of the gene should be
incorporated into the bacterial genome. No promoter is
necessary to transcribe a transformed gene.
b) The expressed gene must be incorporated into the bacterial
chromosome, but its promoter region should be replaced with a
bacterial promoter.
RNA Polymerase II by itself does not have a strong affinity for
binding DNA. Select the explanation that describes why cells do
,not need for RNA polymerase II to have a strong affinity for
binding DNA for transcription to occur.
The requirement of general transcription factors to bind RNA
polymerase II to the DNA molecule gives the cell more precise
control over transcription.
TFIID binds to
a) DNA
b) activator
c) DNA polymerase II
d) all of the above
d) all of the above
A regulatory element that can function from a large distance
(e.g. tens of thousands of base pairs) from a promoter is called
an enhancer
How could an activator influence gene expression at a promoter
far away from the place that it binds DNA?
A bend in the DNA allows the activator-coactivator to interact
with mediator and mediator stimulates transcription.
Which of the following statements about enhancers is TRUE?
a) Enhancers are sequences to which activators bind.
, b) Enhancers are found in the promoter of a gene.
c) Enhancers are only found in certain chromosomal locations.
d) Enhancers are sequences to which activators bind and they
are found in the promoter of a gene.
e) Enhancers are found in the promoter of a gene, and they are
only found in certain chromosomal locations.
a) Enhancers are sequences to which activators bind.
what protein would not interact with a coactivator?
repressors
why do eukaryotes use so many regulatory elements, such as
activators, repressors, coactivators, and basal transcription
factors?
The complexity allows specific control over the timing and
quantity of the protein produced.
what are the the consequence of an activator with a loss-of-
function mutation on expression of a transcriptional target gene?
The gene will be expressed, but in less than optimal quantities.
DNA is associated with proteins to form
chromatin
a _______ is composed of DNA wrapped around an octamer of
histone proteins
nucleosome
It is often desirable to express eukaryotic genes in bacteria,
which can make a protein of interest quickly and cheaply. What
is TRUE about this kind of genetic transformation?
a) The expressed gene and the genes for all of its regulatory
elements must be incorporated into the bacterial chromosome.
b) The expressed gene must be incorporated into the bacterial
chromosome, but its promoter region should be replaced with a
bacterial promoter.
c) Only polycistronic genes are capable of being expressed in a
bacteria.
d) The expressed gene and the gene for RNA Polymerase II
must both be incorporated into the bacterial genome.
e) Only the transcribed region of the gene should be
incorporated into the bacterial genome. No promoter is
necessary to transcribe a transformed gene.
b) The expressed gene must be incorporated into the bacterial
chromosome, but its promoter region should be replaced with a
bacterial promoter.
RNA Polymerase II by itself does not have a strong affinity for
binding DNA. Select the explanation that describes why cells do
,not need for RNA polymerase II to have a strong affinity for
binding DNA for transcription to occur.
The requirement of general transcription factors to bind RNA
polymerase II to the DNA molecule gives the cell more precise
control over transcription.
TFIID binds to
a) DNA
b) activator
c) DNA polymerase II
d) all of the above
d) all of the above
A regulatory element that can function from a large distance
(e.g. tens of thousands of base pairs) from a promoter is called
an enhancer
How could an activator influence gene expression at a promoter
far away from the place that it binds DNA?
A bend in the DNA allows the activator-coactivator to interact
with mediator and mediator stimulates transcription.
Which of the following statements about enhancers is TRUE?
a) Enhancers are sequences to which activators bind.
, b) Enhancers are found in the promoter of a gene.
c) Enhancers are only found in certain chromosomal locations.
d) Enhancers are sequences to which activators bind and they
are found in the promoter of a gene.
e) Enhancers are found in the promoter of a gene, and they are
only found in certain chromosomal locations.
a) Enhancers are sequences to which activators bind.
what protein would not interact with a coactivator?
repressors
why do eukaryotes use so many regulatory elements, such as
activators, repressors, coactivators, and basal transcription
factors?
The complexity allows specific control over the timing and
quantity of the protein produced.
what are the the consequence of an activator with a loss-of-
function mutation on expression of a transcriptional target gene?
The gene will be expressed, but in less than optimal quantities.
DNA is associated with proteins to form
chromatin
a _______ is composed of DNA wrapped around an octamer of
histone proteins
nucleosome
