BMS 127: Lab Practical Final Exam Questions With
Complete Answers
When are you permitted in lab? - ANSWER only when supervised by staff member
Visitors in lab - ANSWER not permitted without special permission
forbidden in lab - ANSWER eating, drinking, smoking, applying lip balm
cell phone use - ANSWER forbidden
accidents/spills - ANSWER reported to instructor immediately
Are gloves optional or required? - ANSWER optional for everyday use
-required and provided for select few
Safety glasses - ANSWER optional
daily routine - ANSWER -stow belongings
-wash hands with antimicrobial soap
-lab coat, hair back
,-bleach bench top
-reverse
Eye-wash and shower station location - ANSWER rear (south exit)
fire extinguishers - ANSWER either side of blackboard
sinks - ANSWER student bench
first aid - ANSWER lab prep area
Red Biohazard containers - ANSWER all disposable materials that have touched
microorganisms
Sharps container - ANSWER front room next to fume hood
-all broken glass,
-toothpicks
-anything that could puncture plastic bag
-anything that could injure a custodian
-MICROSCOPE SLIDES
,Cleaning Spilled Bacterial Culture - ANSWER 1. notify everyone
2. flood spill with lots of bleach
3. cover area with paper towels
4. let sit 10-15 minutes to kill microorganisms
5. wipe up, dispose in biohazard container and sharps
aseptic technique - ANSWER series of procedures designed to minimize contamination of
laboratory workers, their samples, and their surroundings
agar - ANSWER -gelatinous substance,
-mostly polysaccharides
-derived from marine algae
-solid at room temp
-holds lots of H2O and nutrients
-not consumed by microorganisms
broth - ANSWER name for any liquid nutrient solution in which microorganisms can grow
culture tube/petri dish - ANSWER contain cultures
agar slant - ANSWER culture tube containing agar that has solidified at an angle
, -increase SA on which microorgs. will grow
agar deep - ANSWER agar in a culture tube allowed to solidify upright
Why are petri dishes stored upside down? - ANSWER -prevents condensation inside lid from
falling down onto culture
-helps prevent accidental exposure to and contamination of culture within
Bunsen burner - ANSWER
colony of bacteria - ANSWER lump of bacterial growth to see with naked eye
Why is mouth of culture tube flamed and what happens? - ANSWER -decontaminate area to
ensure no cross-contamination between bacteria/loop
-creates a column of rising heat to prevent contamination
Pure culture - ANSWER microbial growth containing just one species
Why can't you place loop directly on bench top? - ANSWER -could be contaminated
Why is hand held higher than loop being flamed? - ANSWER so hot liquids wont burn you
Complete Answers
When are you permitted in lab? - ANSWER only when supervised by staff member
Visitors in lab - ANSWER not permitted without special permission
forbidden in lab - ANSWER eating, drinking, smoking, applying lip balm
cell phone use - ANSWER forbidden
accidents/spills - ANSWER reported to instructor immediately
Are gloves optional or required? - ANSWER optional for everyday use
-required and provided for select few
Safety glasses - ANSWER optional
daily routine - ANSWER -stow belongings
-wash hands with antimicrobial soap
-lab coat, hair back
,-bleach bench top
-reverse
Eye-wash and shower station location - ANSWER rear (south exit)
fire extinguishers - ANSWER either side of blackboard
sinks - ANSWER student bench
first aid - ANSWER lab prep area
Red Biohazard containers - ANSWER all disposable materials that have touched
microorganisms
Sharps container - ANSWER front room next to fume hood
-all broken glass,
-toothpicks
-anything that could puncture plastic bag
-anything that could injure a custodian
-MICROSCOPE SLIDES
,Cleaning Spilled Bacterial Culture - ANSWER 1. notify everyone
2. flood spill with lots of bleach
3. cover area with paper towels
4. let sit 10-15 minutes to kill microorganisms
5. wipe up, dispose in biohazard container and sharps
aseptic technique - ANSWER series of procedures designed to minimize contamination of
laboratory workers, their samples, and their surroundings
agar - ANSWER -gelatinous substance,
-mostly polysaccharides
-derived from marine algae
-solid at room temp
-holds lots of H2O and nutrients
-not consumed by microorganisms
broth - ANSWER name for any liquid nutrient solution in which microorganisms can grow
culture tube/petri dish - ANSWER contain cultures
agar slant - ANSWER culture tube containing agar that has solidified at an angle
, -increase SA on which microorgs. will grow
agar deep - ANSWER agar in a culture tube allowed to solidify upright
Why are petri dishes stored upside down? - ANSWER -prevents condensation inside lid from
falling down onto culture
-helps prevent accidental exposure to and contamination of culture within
Bunsen burner - ANSWER
colony of bacteria - ANSWER lump of bacterial growth to see with naked eye
Why is mouth of culture tube flamed and what happens? - ANSWER -decontaminate area to
ensure no cross-contamination between bacteria/loop
-creates a column of rising heat to prevent contamination
Pure culture - ANSWER microbial growth containing just one species
Why can't you place loop directly on bench top? - ANSWER -could be contaminated
Why is hand held higher than loop being flamed? - ANSWER so hot liquids wont burn you
