BIO 198 Lab Practical - Final Exam
Study Guide
what are the different levels of protein structure? - Answer-primary structure
secondary structure
tertiary structure
quaternary structure
primary structure - Answer-first level of protein structure, sequence of amino acids linked together to
form a polypeptide chain
secondary structure - Answer-second level of protein structure, regular and recurring arrangements in
space of adjacent amino acid residues in a polypeptide chain
tertiary structure - Answer-third level, overall three-dimensional arrangement of its polypeptide chain in
space
quaternary structure - Answer-fourth level, association of several protein chains or subunits into a
closely packed arrangement
relationship between enzymes, substrates, and products - Answer-A substrate enters the active site of
an enzyme; the reaction then occurs, converting the substrate into products and forming an enzyme
products complex. The products then leave the active site of the enzyme
what are some environmental factors that can affect the structure of a protein? - Answer-pH and
temperature
, what does it mean when an enzyme is denatured? - Answer-causes bonds to be broken, structure to be
disrupted and it stops functioning
- happens when enzymes are exposed to extreme conditions (high temp or extreme pH levels)
how does temperature effect the rate of peroxidase - Answer-10 to 30 degrees: activity increased slowly
30 degrees and above: rate of activity increases
70 degrees: reaches its peak
how was the rate of enzyme calculated - Answer-slope/time
peroxidase - Answer-enzyme used in the experiment, breaks down H202
Equation for cellular respiration - Answer-C6H12O6 + 6O2 --> 6CO2 + 6H2O + energy
What organisms were tested in Exercise 6? - Answer-germinating peas and superworms
what was measured in order to determine the rate of respiration in the peas and superworms? -
Answer-measured how much CO2 was released, inserted the CO2 sensor in the neck of the chamber
(connected to LabQuest)
M.A.R
units? - Answer-Mass-Adjusted Rate
- divide the rates of carbon dioxide production by the mass of the organism
- ppm/s/g
Study Guide
what are the different levels of protein structure? - Answer-primary structure
secondary structure
tertiary structure
quaternary structure
primary structure - Answer-first level of protein structure, sequence of amino acids linked together to
form a polypeptide chain
secondary structure - Answer-second level of protein structure, regular and recurring arrangements in
space of adjacent amino acid residues in a polypeptide chain
tertiary structure - Answer-third level, overall three-dimensional arrangement of its polypeptide chain in
space
quaternary structure - Answer-fourth level, association of several protein chains or subunits into a
closely packed arrangement
relationship between enzymes, substrates, and products - Answer-A substrate enters the active site of
an enzyme; the reaction then occurs, converting the substrate into products and forming an enzyme
products complex. The products then leave the active site of the enzyme
what are some environmental factors that can affect the structure of a protein? - Answer-pH and
temperature
, what does it mean when an enzyme is denatured? - Answer-causes bonds to be broken, structure to be
disrupted and it stops functioning
- happens when enzymes are exposed to extreme conditions (high temp or extreme pH levels)
how does temperature effect the rate of peroxidase - Answer-10 to 30 degrees: activity increased slowly
30 degrees and above: rate of activity increases
70 degrees: reaches its peak
how was the rate of enzyme calculated - Answer-slope/time
peroxidase - Answer-enzyme used in the experiment, breaks down H202
Equation for cellular respiration - Answer-C6H12O6 + 6O2 --> 6CO2 + 6H2O + energy
What organisms were tested in Exercise 6? - Answer-germinating peas and superworms
what was measured in order to determine the rate of respiration in the peas and superworms? -
Answer-measured how much CO2 was released, inserted the CO2 sensor in the neck of the chamber
(connected to LabQuest)
M.A.R
units? - Answer-Mass-Adjusted Rate
- divide the rates of carbon dioxide production by the mass of the organism
- ppm/s/g
