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BIO 340 Midterm Exam 2025 – Complete Biology
Study Guide, Practice Test Questions & Answer Key
Prepare for your BIO 340 Midterm Exam with this comprehensive 2025 biology study guide.
Covers genetics, molecular biology, cell structure, and physiology with practice questions,
answers, and detailed explanations to help you master every topic and boost your exam score.
BIO 340 Midterm Exam,
BIO 340 study guide,
BIO 340 midterm review,
BIO 340 practice test,
Which of the following statements about the column chromatography is not correct? - answer-gel
filtration chromatography separates proteins on their ability to bind to specific groups on the column
matrix
Which of the following is true about sds page - answer-it involves protein denaturation
Which of the following is the primary factor that dictates how far a protein will migrate during sds-page -
answer-size
Which of the following statements about western blotting is correct? - answer-the detection of a
particular protein by western blotting relies on the very specific interaction between the protein and
antibody
X ray diffraction - answer-- more suitable for complexes larger than about 100kd
, 2|Page
- requires samples to crystallize
Nuclear magnetic resonance nmr - answer-better reveal dynamics of molecular motions
Nmr and x - answer-used to determine structure of proteins
Which of the following statements about the use of mass spectrometry in protein investigation is not
correct? - answer-mass spectrometry involves the separation of ionic fragments on a gel
Which of the following would require an electron microscope in order to be viewed? - answer-a
ribosome
In which of the following microscopy techniques are oblique rays of light focused on the specimen? -
answer-dark-field microscopy
A bacterial enzyme that cuts dna within a short, specific sequence and is used in many applications by
the molecular biologist is known as: - answer-restriction enzymes
Which of the following is not needed in order to amplify a dna fragment by pcr - answer-knowledge of
the complete nucleotide sequence of the fragment to be amplified
Which of the following best describes the complete sequence of steps occurring every cycle of pcr? -
answer-the primers hybridize to the target dna
Which is the function of dideoxynucelotides in sanger dna sequencing? - answer-they stop synthesis at a
specific site, so the base at that site can be determined
Illumina next generation sequencing uses... - answer-light detection after each round of synthesis
Which of the following is an advantage of rna sequencing over microarray analysis when looking at the
mrna expression levels within a population of cells? - answer-you can quantify all the rna, rather than
just the previously defines transcripts genes that you have probes for
BIO 340 Midterm Exam 2025 – Complete Biology
Study Guide, Practice Test Questions & Answer Key
Prepare for your BIO 340 Midterm Exam with this comprehensive 2025 biology study guide.
Covers genetics, molecular biology, cell structure, and physiology with practice questions,
answers, and detailed explanations to help you master every topic and boost your exam score.
BIO 340 Midterm Exam,
BIO 340 study guide,
BIO 340 midterm review,
BIO 340 practice test,
Which of the following statements about the column chromatography is not correct? - answer-gel
filtration chromatography separates proteins on their ability to bind to specific groups on the column
matrix
Which of the following is true about sds page - answer-it involves protein denaturation
Which of the following is the primary factor that dictates how far a protein will migrate during sds-page -
answer-size
Which of the following statements about western blotting is correct? - answer-the detection of a
particular protein by western blotting relies on the very specific interaction between the protein and
antibody
X ray diffraction - answer-- more suitable for complexes larger than about 100kd
, 2|Page
- requires samples to crystallize
Nuclear magnetic resonance nmr - answer-better reveal dynamics of molecular motions
Nmr and x - answer-used to determine structure of proteins
Which of the following statements about the use of mass spectrometry in protein investigation is not
correct? - answer-mass spectrometry involves the separation of ionic fragments on a gel
Which of the following would require an electron microscope in order to be viewed? - answer-a
ribosome
In which of the following microscopy techniques are oblique rays of light focused on the specimen? -
answer-dark-field microscopy
A bacterial enzyme that cuts dna within a short, specific sequence and is used in many applications by
the molecular biologist is known as: - answer-restriction enzymes
Which of the following is not needed in order to amplify a dna fragment by pcr - answer-knowledge of
the complete nucleotide sequence of the fragment to be amplified
Which of the following best describes the complete sequence of steps occurring every cycle of pcr? -
answer-the primers hybridize to the target dna
Which is the function of dideoxynucelotides in sanger dna sequencing? - answer-they stop synthesis at a
specific site, so the base at that site can be determined
Illumina next generation sequencing uses... - answer-light detection after each round of synthesis
Which of the following is an advantage of rna sequencing over microarray analysis when looking at the
mrna expression levels within a population of cells? - answer-you can quantify all the rna, rather than
just the previously defines transcripts genes that you have probes for
