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Molecular & Cellular Biology Exam questions and answers graded A+

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Molecular & Cellular Biology Exam questions and answers graded A+

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Molecular & Cellular Biology Exam
questions and answers graded A+
what does the "P" in "PCR" stand for? - ANS✅✅polymerase

what does the "C" in "PCR" stand for? - ANS✅✅chain

what does the "R" in "PCR" stand for? - ANS✅✅reaction

what enzyme catalyzes PCR? - ANS✅✅DNA polymerase

how many steps are in 1 cycle of PCR? - ANS✅✅3

if you start with 1 copy of DNA, how many copies should there be after 4 cycles of PCR? -
ANS✅✅16

how many primers are typically needed for PCR? - ANS✅✅2

for the denaturation stepf of PCR, approximately what temperature is used? - ANS✅✅96 C

denaturing DNA means... - ANS✅✅breaking the hydrogen bonds between the bases

what metal ion is essential for PCR? - ANS✅✅Mg2+

when primers bind to their complementary sequences it is called... - ANS✅✅annealing

primers are extended... - ANS✅✅only 5' to 3' direction

what enzyme is used in PCR during the first cycle to add nucleotides to the 2nd strand? -
ANS✅✅tac polymerase

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Molecular & Cellular Biology Exam questions and answers graded A+

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