UAMS Micro Exam 5 UPDATED ACTUAL Questions and CORRECT Answers
Blastomyces dermatitidis
Identify the following organism: broad
based budding yeast (figure 8) on KOH
mount
What is the purpose of using KOH? Breaks down cellular material; thus allows the viewing of fungal elements
What is the recommended temperature & 25-30; 4 weeks
the length of time for incubating fungal
cultures?
Which tests are used to identify the Conversion of yeast to mold form, exoantigens, & NA probes (method of choice)
dimorphic molds?
What is the preferred temperature for 25-33
growing M. marinum, M. haemophilum & M.
ulcerans
Which test has replaced the India ink mount Cryptococcus antigen test - more sensitive
on CSF?
What is the purpose of using the Typically used to confirm a positive fluorochrome stain
Kinyoun/Ziehl-Neelson stain?
What is the disease caused by White piedra
Trichosporon beigelii?
Describe how to perform an India ink CSF + India ink; coverslip & read
mount.
What is the principle of the BACTEC 460 Measure 14CO2 production
system?
Which fungus is endemic in the American Coccidioides immitis
Southwest?
, Buff, rough colonies
Describe the appearance of MTB on LJ
medium.
What is the principle of the VersaTrek Measures pressure changes (manometric system)
(formerly ESP Myco) system?
Which mycobacterial media are considered Middlebrook agar: 7H10 & & 7H11
nonselective agar-based media?
"Beaver-tail" shape
Describe the macroconidia associated with
Epidermophyton?
Identify the reagents used in the Auramine-o-rhodamine, acid alcohol, & potassium permanganate
fluorescent stain.
Describe how to perform a chlamydospore Inoculate cornmeal agar; coverslip & incubate; examine for chlamydospores
test.
Identify the following organism: niacin & M. tuberculosis (MTB)
nitrate +, 68 C catalase =, & buff, rough
colonies
Actinomycotic mycetoma are caused by Actinomycetes, like Nocardia, Streptomyces, or Actinomadura
_______
Food-absorbing portion of the hyphae; grows into
agar
Define vegetative hyphae.
What diseases are caused Nocardia? Nocardiosis (pulmonary) & mycetoma (granules present)
While examining a Gram stain smear from a Perform a modified AF stain
brain abscess, the MT sees Gram-positive
branching rods. Before contacting the
doctor, the technologist should ______
Define flavus. Septate hyphae in hair
How do the interferon-gamma release Measures release of interferon gamma by blood WBCs to MTB antigens
assays differ from the TB skin test?
Describe how to perform a KOH mount. Specimen + 10% KOH; coverslip & heat gently; read after 10-15 minutes
What are the recommended collection AM sputum on 3 successive days
procedures for isolating fungi from
sputum?
Which disease causes "cauliflower-like Chromoblastomycosis
lesions" usually found on extremities?
Which organism is considered the tap M. gordonae
water scotochromogen?
Identify the following organism: thick Coccidioides immitis
walled spherule w/ endospores on KOH &
alternating arthroconidia on LPCB mount
Blastomyces dermatitidis
Identify the following organism: broad
based budding yeast (figure 8) on KOH
mount
What is the purpose of using KOH? Breaks down cellular material; thus allows the viewing of fungal elements
What is the recommended temperature & 25-30; 4 weeks
the length of time for incubating fungal
cultures?
Which tests are used to identify the Conversion of yeast to mold form, exoantigens, & NA probes (method of choice)
dimorphic molds?
What is the preferred temperature for 25-33
growing M. marinum, M. haemophilum & M.
ulcerans
Which test has replaced the India ink mount Cryptococcus antigen test - more sensitive
on CSF?
What is the purpose of using the Typically used to confirm a positive fluorochrome stain
Kinyoun/Ziehl-Neelson stain?
What is the disease caused by White piedra
Trichosporon beigelii?
Describe how to perform an India ink CSF + India ink; coverslip & read
mount.
What is the principle of the BACTEC 460 Measure 14CO2 production
system?
Which fungus is endemic in the American Coccidioides immitis
Southwest?
, Buff, rough colonies
Describe the appearance of MTB on LJ
medium.
What is the principle of the VersaTrek Measures pressure changes (manometric system)
(formerly ESP Myco) system?
Which mycobacterial media are considered Middlebrook agar: 7H10 & & 7H11
nonselective agar-based media?
"Beaver-tail" shape
Describe the macroconidia associated with
Epidermophyton?
Identify the reagents used in the Auramine-o-rhodamine, acid alcohol, & potassium permanganate
fluorescent stain.
Describe how to perform a chlamydospore Inoculate cornmeal agar; coverslip & incubate; examine for chlamydospores
test.
Identify the following organism: niacin & M. tuberculosis (MTB)
nitrate +, 68 C catalase =, & buff, rough
colonies
Actinomycotic mycetoma are caused by Actinomycetes, like Nocardia, Streptomyces, or Actinomadura
_______
Food-absorbing portion of the hyphae; grows into
agar
Define vegetative hyphae.
What diseases are caused Nocardia? Nocardiosis (pulmonary) & mycetoma (granules present)
While examining a Gram stain smear from a Perform a modified AF stain
brain abscess, the MT sees Gram-positive
branching rods. Before contacting the
doctor, the technologist should ______
Define flavus. Septate hyphae in hair
How do the interferon-gamma release Measures release of interferon gamma by blood WBCs to MTB antigens
assays differ from the TB skin test?
Describe how to perform a KOH mount. Specimen + 10% KOH; coverslip & heat gently; read after 10-15 minutes
What are the recommended collection AM sputum on 3 successive days
procedures for isolating fungi from
sputum?
Which disease causes "cauliflower-like Chromoblastomycosis
lesions" usually found on extremities?
Which organism is considered the tap M. gordonae
water scotochromogen?
Identify the following organism: thick Coccidioides immitis
walled spherule w/ endospores on KOH &
alternating arthroconidia on LPCB mount
