UAMS Micro exam 4 UPDATED ACTUAL Questions and CORRECT Answers
corresponds to the flora in the mothers vagina
Normal Flora at birth
usually micrococci, streptococci, enterics, and lactobacillus
The bladder and urine is usually Sterile
MO's found in urine are usually contaminants
Always correlate the gram stain with what was cultured
The most important step is specimen procurement
, Best urine specimen 1st morning urine
best blood culture specimen collected at time of fever, before antibiotics
a lab test is no better than the specimen collected
preferred specimen transport time to the 2 hours
lab
how are sputum and urine stored refridgerated
how are CFS and other sterile fluids stored roomtemp
wounds, abscesses, sputum, Male urethral swab, sterile body fluids, Stool for WBCs
routinely gramstained specimens
only
high PMNs - indicates infection
Determine quality of specimen
low Epis - (high epis indicate contamination)
Enumerate cells 10x
Enumerate bacteria 100x
1-10 1+
Examine 30 fields
11-24 2+
what are the grading values
>= 25 3+
you can be more specific and make a more Blood culture
"probable" identification on what specimen
urine
vagina/throat/rectal for GC
inappropriate to gram stain
Throat for GAS
Stool for enteric pathogens (salmonella/shigella)
lack of normal flora during food poisoning - staph
exceptions for inappropriate stool gram
specific rod morphology for Campylobacter
stain rules
WBC count (PMNs)
CO2 - for fastidious MOs
incubate media in
facultative MOs will grow anyway
BAP, CHOC, Broth
Media for Normally Sterile body fluids anaerobic if requested
MAC/EMB if requested
stains nucleic acids
Acridine Orange used for NSBF
bacteria and yeast fluoresce orange
H. influenzae type B - children <2
Strep Pneumo - children, elderly, head trauma, immunocompromised
common organisms for NSBF Neisseria Meningitidis - children
GBS - infants
Listeria, Staph
tissue/biopsy - ground up
specimen types for wound/abcess pus - best specimen
swab - least desirable specimen
S. aureus
GAS
Enterics
most common wound/abcess MO
Anaerobes
PSAR
Enterococci
corresponds to the flora in the mothers vagina
Normal Flora at birth
usually micrococci, streptococci, enterics, and lactobacillus
The bladder and urine is usually Sterile
MO's found in urine are usually contaminants
Always correlate the gram stain with what was cultured
The most important step is specimen procurement
, Best urine specimen 1st morning urine
best blood culture specimen collected at time of fever, before antibiotics
a lab test is no better than the specimen collected
preferred specimen transport time to the 2 hours
lab
how are sputum and urine stored refridgerated
how are CFS and other sterile fluids stored roomtemp
wounds, abscesses, sputum, Male urethral swab, sterile body fluids, Stool for WBCs
routinely gramstained specimens
only
high PMNs - indicates infection
Determine quality of specimen
low Epis - (high epis indicate contamination)
Enumerate cells 10x
Enumerate bacteria 100x
1-10 1+
Examine 30 fields
11-24 2+
what are the grading values
>= 25 3+
you can be more specific and make a more Blood culture
"probable" identification on what specimen
urine
vagina/throat/rectal for GC
inappropriate to gram stain
Throat for GAS
Stool for enteric pathogens (salmonella/shigella)
lack of normal flora during food poisoning - staph
exceptions for inappropriate stool gram
specific rod morphology for Campylobacter
stain rules
WBC count (PMNs)
CO2 - for fastidious MOs
incubate media in
facultative MOs will grow anyway
BAP, CHOC, Broth
Media for Normally Sterile body fluids anaerobic if requested
MAC/EMB if requested
stains nucleic acids
Acridine Orange used for NSBF
bacteria and yeast fluoresce orange
H. influenzae type B - children <2
Strep Pneumo - children, elderly, head trauma, immunocompromised
common organisms for NSBF Neisseria Meningitidis - children
GBS - infants
Listeria, Staph
tissue/biopsy - ground up
specimen types for wound/abcess pus - best specimen
swab - least desirable specimen
S. aureus
GAS
Enterics
most common wound/abcess MO
Anaerobes
PSAR
Enterococci
