UAMS MICRO EXAM 3 UPDATED ACTUAL Questions and CORRECT Answers
Open tube: Acid (yellow)
Closed tube: Acid (yellow)
OF Reaction for Fermenters
Ex: Enterobacteriacea
Open tube: Acid (yellow)
Closed tube: No acid (green/blue)
OF Reaction for Oxidizers
Ex: Pseudomonas aeruginosa
Open tube: No acid (green/blue)
Closed tube: No acid (green/blue)
OF Reaction for Non-oxidizers
Ex: Assachrolytic Acinetobacter
(+) result; red color within 30 min of addition of Reagents A and B.
Nitrate Reduction Test:
Nitrate reduced to Nitrite.
Ex. E. coli.
(+) results. Red color does not appear with addition of Reagents A and B. Medium
Nitrate Reduction Test: stays colorless when zinc dust is added.
Nitrogen gas produced.
Ex: Pseudomonas aeruginosa.
A negative result. A red color appears after the addition of zinc dust.
Nitrate Reduction Test:
Nitrate not reduced.
Ex: Acinetobacter.
Causes diseases such as swimmer's ear and infections in immunocompromised
Pseudomonas aeruginosa Diseases
patients (CF and burn patients).
Morphology: Large, irregularly shaped, metallic sheen. Produces Pyocyanin. Beta
Pseudomonas aeruginosa Morphology hemolytic on sheep BAP; Grows on MAC (lactose neg).
Grows on routine media including MAC.
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, Glucose oxidizer
Oxidase positive
P. aeruginosa Key ID Tests Growth at 42 degrees
TSI: A/NC
Produces pyocyanin
Pseudomonas aeruginosa What is the most commonly isolated NFB?
Acinetobacter What is the 2nd most commonly isolated NFB?
Which species of Acinetobacter are A. baumannii = saccharolytic
saccharolytic and which are A. lwoffii = assaccharolytic
assacharolytic?
Oxidase negative
Acinetobacter Key ID Tests Nitrate negative
Glucose utilization
Which microbe can appear as pairs of Acinetobacter
plump coccobacilli or as filamentous rods?
What is the 3rd most commonly isolated Stenotrophomonas maltophilia
NFB?
Oxidase negative
Growth on MAC
Key ID for Stenotrophomonas maltophilia
Uses glucose and maltose
Sensitive to SXT
Important nosocomial pathogen
Diseases of Burkholderia cepacia
Cepacia syndrome: severe resp disease in CF patients
What color is B. cepacia on BCSA and Yellow - produces acid from lactose
OFPBL agar?
What microbe has a strong, earthy odor? Burkholderia cepacia (and pseudomallei)
Oxidase positive (weak)
ID for B. cepacia
Bipolar staining on Gram stain
What microbe causes melioidosis, an Burkholderia pseudomallei
aggressive granulomatous pulmonary
disease?
What microbe is known as the Vietnamese B. pseudomallei
time bomb?
What microbe produces wrinkled colonies B. pseudomallei
on lab media?
ID for B. pseudomallei Oxidase positive (weak)
Pairs of plump coccobacilli
Penicillin test is used to differentiate short coccobacilli to true cocci.
Moraxella characteristics on Gram stain
-coccobacilli will form rods
-true cocci will remain as cocci
Grows on blood agar
Culture characteristics of Moraxella MAC growth is variable
Usually don't need to ID species
Oxidase positive
Key characteristics of Moraxella Asaccharolytic
Penicillin susceptible (most NFB are resistant)
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Open tube: Acid (yellow)
Closed tube: Acid (yellow)
OF Reaction for Fermenters
Ex: Enterobacteriacea
Open tube: Acid (yellow)
Closed tube: No acid (green/blue)
OF Reaction for Oxidizers
Ex: Pseudomonas aeruginosa
Open tube: No acid (green/blue)
Closed tube: No acid (green/blue)
OF Reaction for Non-oxidizers
Ex: Assachrolytic Acinetobacter
(+) result; red color within 30 min of addition of Reagents A and B.
Nitrate Reduction Test:
Nitrate reduced to Nitrite.
Ex. E. coli.
(+) results. Red color does not appear with addition of Reagents A and B. Medium
Nitrate Reduction Test: stays colorless when zinc dust is added.
Nitrogen gas produced.
Ex: Pseudomonas aeruginosa.
A negative result. A red color appears after the addition of zinc dust.
Nitrate Reduction Test:
Nitrate not reduced.
Ex: Acinetobacter.
Causes diseases such as swimmer's ear and infections in immunocompromised
Pseudomonas aeruginosa Diseases
patients (CF and burn patients).
Morphology: Large, irregularly shaped, metallic sheen. Produces Pyocyanin. Beta
Pseudomonas aeruginosa Morphology hemolytic on sheep BAP; Grows on MAC (lactose neg).
Grows on routine media including MAC.
https://quizlet.com/893025857/uams-micro-exam-3-flash-cards/ 1/7
, Glucose oxidizer
Oxidase positive
P. aeruginosa Key ID Tests Growth at 42 degrees
TSI: A/NC
Produces pyocyanin
Pseudomonas aeruginosa What is the most commonly isolated NFB?
Acinetobacter What is the 2nd most commonly isolated NFB?
Which species of Acinetobacter are A. baumannii = saccharolytic
saccharolytic and which are A. lwoffii = assaccharolytic
assacharolytic?
Oxidase negative
Acinetobacter Key ID Tests Nitrate negative
Glucose utilization
Which microbe can appear as pairs of Acinetobacter
plump coccobacilli or as filamentous rods?
What is the 3rd most commonly isolated Stenotrophomonas maltophilia
NFB?
Oxidase negative
Growth on MAC
Key ID for Stenotrophomonas maltophilia
Uses glucose and maltose
Sensitive to SXT
Important nosocomial pathogen
Diseases of Burkholderia cepacia
Cepacia syndrome: severe resp disease in CF patients
What color is B. cepacia on BCSA and Yellow - produces acid from lactose
OFPBL agar?
What microbe has a strong, earthy odor? Burkholderia cepacia (and pseudomallei)
Oxidase positive (weak)
ID for B. cepacia
Bipolar staining on Gram stain
What microbe causes melioidosis, an Burkholderia pseudomallei
aggressive granulomatous pulmonary
disease?
What microbe is known as the Vietnamese B. pseudomallei
time bomb?
What microbe produces wrinkled colonies B. pseudomallei
on lab media?
ID for B. pseudomallei Oxidase positive (weak)
Pairs of plump coccobacilli
Penicillin test is used to differentiate short coccobacilli to true cocci.
Moraxella characteristics on Gram stain
-coccobacilli will form rods
-true cocci will remain as cocci
Grows on blood agar
Culture characteristics of Moraxella MAC growth is variable
Usually don't need to ID species
Oxidase positive
Key characteristics of Moraxella Asaccharolytic
Penicillin susceptible (most NFB are resistant)
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