UAMS Micro Exam 3 UPDATED Questions
and CORRECT Answers
What type of reaction is expected when Pseudomonas aeruginosa is inoculated to OF glucose
media? - CORRECT ANSWER - Yellow in open tube
blue-green in closed tube (oil)
Which organism pits the agar? - CORRECT ANSWER - Eikenella corrodens
What is the method of choice for detecting Chlamydia trachomatis in adults? - CORRECT
ANSWER - NAAT
What is the appearance of nonfermenters on MAC/EMB? - CORRECT ANSWER -
Lactose negative - colorless
Vibrio is usually transmitted through contaminated ________ & _________. - CORRECT
ANSWER - Water & seafood
What is the Gram stain result for Actinomyces? - CORRECT ANSWER - Gram positive
branching rods
What type of specimens are acceptable for anaerobic cultures? - CORRECT ANSWER -
Aspirates & tissues; swabs are least desirable
Which organism appears green on TSA and why? - CORRECT ANSWER - Pseudomonas
aeruginosa produces pyocyanin
Which organism is associated with lice? - CORRECT ANSWER - Borrelia recurrentis
,What is the method of choice for detecting Bordetella & Ehrlichia/Anaplasma? - CORRECT
ANSWER - PCR
What type of nitrate reaction is produced by Pseudomonas aeruginosa? - CORRECT
ANSWER - Positive; no color change after addition of zinc (nitrate to nitrogen gas)
Name the STD caused by Chlamydia trachomatis that produces a regional lymphadenopathy
(hint: the disease is rarely seen in the U.S.). - CORRECT ANSWER - Lymphogranuloma
venereum
Although Actinobacillus actinomycetemcomitans is an aerobic organism, it is often found in
association with an anerobic organism. What is that MO? - CORRECT ANSWER -
Actinomyces israelii
Which organism is found in animal urine? - CORRECT ANSWER - Leptospira
What is the medium used to isolate Legionella? - CORRECT ANSWER - BCYE
Name the diseases caused by Chlamydia trachomatis that effect newborns. - CORRECT
ANSWER - Inclusion conjunctivitis & neonatal pneumonia
What two methods are used to detect Chlamydia trachomatis in shell vials? - CORRECT
ANSWER - Iodine stain for glycogen inclusion or DFA
Name two nonfermentors that are oxidase negative. - CORRECT ANSWER -
Acinetobacter & Stenotrophomonas
What organisms are used to perform the reverse CAMP test? - CORRECT ANSWER -
Known Gp B strep & suspected Clostridium perfringens isolate
, Which organism causes human granulocyte anaplasmosis? - CORRECT ANSWER -
Anaplasma phagocytophilum
Which organism causes antibiotic associated diarrhea & pesudomembranous colitis? -
CORRECT ANSWER - Clostridium difficile
What is the Gram stain result for Vibro? - CORRECT ANSWER - Gram negative rods;
some may be curved
What is the Gram stain result for the nonfermenters? - CORRECT ANSWER - Gram
negative bacilli
Which organisms are associated with ticks? - CORRECT ANSWER - Rickettsia,
Ehrlichia, Anaplasma, & Borrelia burgdorferi
Burkholderia, Moraxella, Kingella, & Eikenella are all oxidase positive. Name other organisms
that are oxidase positive. - CORRECT ANSWER - Vibrio, Campy/Helico, Aeromonas, &
Plesiomonas (also Pseudomonas)
Which organism causes cat scratch fever, bacteremia, bacillary angiomatosis? - CORRECT
ANSWER - Bartonella henselae
What does BCYE contain that is required for Legionella growth? - CORRECT
ANSWER - Cysteine & iron
What is the identification of the following organism: Double zone of hemolysis
Reverse CAMP +
lecithinase +
Nagler + - CORRECT ANSWER - Clostridium perfringens
and CORRECT Answers
What type of reaction is expected when Pseudomonas aeruginosa is inoculated to OF glucose
media? - CORRECT ANSWER - Yellow in open tube
blue-green in closed tube (oil)
Which organism pits the agar? - CORRECT ANSWER - Eikenella corrodens
What is the method of choice for detecting Chlamydia trachomatis in adults? - CORRECT
ANSWER - NAAT
What is the appearance of nonfermenters on MAC/EMB? - CORRECT ANSWER -
Lactose negative - colorless
Vibrio is usually transmitted through contaminated ________ & _________. - CORRECT
ANSWER - Water & seafood
What is the Gram stain result for Actinomyces? - CORRECT ANSWER - Gram positive
branching rods
What type of specimens are acceptable for anaerobic cultures? - CORRECT ANSWER -
Aspirates & tissues; swabs are least desirable
Which organism appears green on TSA and why? - CORRECT ANSWER - Pseudomonas
aeruginosa produces pyocyanin
Which organism is associated with lice? - CORRECT ANSWER - Borrelia recurrentis
,What is the method of choice for detecting Bordetella & Ehrlichia/Anaplasma? - CORRECT
ANSWER - PCR
What type of nitrate reaction is produced by Pseudomonas aeruginosa? - CORRECT
ANSWER - Positive; no color change after addition of zinc (nitrate to nitrogen gas)
Name the STD caused by Chlamydia trachomatis that produces a regional lymphadenopathy
(hint: the disease is rarely seen in the U.S.). - CORRECT ANSWER - Lymphogranuloma
venereum
Although Actinobacillus actinomycetemcomitans is an aerobic organism, it is often found in
association with an anerobic organism. What is that MO? - CORRECT ANSWER -
Actinomyces israelii
Which organism is found in animal urine? - CORRECT ANSWER - Leptospira
What is the medium used to isolate Legionella? - CORRECT ANSWER - BCYE
Name the diseases caused by Chlamydia trachomatis that effect newborns. - CORRECT
ANSWER - Inclusion conjunctivitis & neonatal pneumonia
What two methods are used to detect Chlamydia trachomatis in shell vials? - CORRECT
ANSWER - Iodine stain for glycogen inclusion or DFA
Name two nonfermentors that are oxidase negative. - CORRECT ANSWER -
Acinetobacter & Stenotrophomonas
What organisms are used to perform the reverse CAMP test? - CORRECT ANSWER -
Known Gp B strep & suspected Clostridium perfringens isolate
, Which organism causes human granulocyte anaplasmosis? - CORRECT ANSWER -
Anaplasma phagocytophilum
Which organism causes antibiotic associated diarrhea & pesudomembranous colitis? -
CORRECT ANSWER - Clostridium difficile
What is the Gram stain result for Vibro? - CORRECT ANSWER - Gram negative rods;
some may be curved
What is the Gram stain result for the nonfermenters? - CORRECT ANSWER - Gram
negative bacilli
Which organisms are associated with ticks? - CORRECT ANSWER - Rickettsia,
Ehrlichia, Anaplasma, & Borrelia burgdorferi
Burkholderia, Moraxella, Kingella, & Eikenella are all oxidase positive. Name other organisms
that are oxidase positive. - CORRECT ANSWER - Vibrio, Campy/Helico, Aeromonas, &
Plesiomonas (also Pseudomonas)
Which organism causes cat scratch fever, bacteremia, bacillary angiomatosis? - CORRECT
ANSWER - Bartonella henselae
What does BCYE contain that is required for Legionella growth? - CORRECT
ANSWER - Cysteine & iron
What is the identification of the following organism: Double zone of hemolysis
Reverse CAMP +
lecithinase +
Nagler + - CORRECT ANSWER - Clostridium perfringens
