Microbiology Lab Questions With Complete Solutions
there are many types of staining tech used in microbiology. which of the following is not a commonly
used staining - (ANSWER)wright's stain
which of the following stains do not require heat fixation? - (ANSWER)capsule stain
The ___ stain provides critical information about peptidoglycan structure of the cell wall. -
(ANSWER)Gram
Which of the following stains require heat fixation? - (ANSWER)Endospore
Gram
Simple
Acid-fast
Gram stain - (ANSWER)a stain that can differentiate between cells that have thick or thin layers of
peptidoglycan in their cell walls
Acid-Fast stain - (ANSWER)a stain that would be appropriate for cells that contain mycolic acid in their
cell walls.
Capsule stain - (ANSWER)a stain that enables you to effectively visualize a bacterium's glycocalyx
Which of the following provides a quick and effective method used to visualize and determine bacterial
morphology and arrangement? - (ANSWER)Simple stain
If you decolorize for more than 30 seconds, gram-positive organisms will most likely appear ____ in
color. - (ANSWER)pink
What might happen if you omitted the safranin step while performing the Gram stain on the mixed
culture of gram-positive and gram-negative cells? - (ANSWER)Gram-negative organisms might not be
visible
, Microbiology Lab Questions With Complete Solutions
if you omit the Gram's iodine step while preforming the Gram stain, gram-positive cells will most likely
be ____ in color. - (ANSWER)pink
What outcome could be expected if you forgot the crystal violet step while performing a Gram stain? -
(ANSWER)Gram-positive and gram-negative organisms would stain pink
Gram-negative cells appear __ after Gram staining whereas gram-positive cells appear __. -
(ANSWER)red, purple
If you forget to use the decolorizer, which cell type(s) will appear purple? - (ANSWER)Gram-positive and
Gram-negative
What is the primary dye used in the gram staining technique? - (ANSWER)crystal violet
What is used as the mordant in the gram staining technique? - (ANSWER)Gram's iodine
which of the following correctly describe how the Gram stain works to color different types of cells? -
(ANSWER)gram-positive cells retain the purple dye-mordant complex because of their thick layer of
peptidoglycan.
gram-negative cells are decolorized and stained by the pink/red secondary stain
which of the following describes the correct sequence of the gram stain procedure? - (ANSWER)heat-
fixed slide; use of crystal violet, iodine, decolorizer, and safranin
which of the following are terms used to describe the colored dyes applied in the Gram staining
technique? - (ANSWER)primary and counterstain
what is thought to cause gram-positive cells to retain the primary stain and the mordant complex
whereas gram-negative cells are decolorized? - (ANSWER)the thickness of the peptidoglycan layer
there are many types of staining tech used in microbiology. which of the following is not a commonly
used staining - (ANSWER)wright's stain
which of the following stains do not require heat fixation? - (ANSWER)capsule stain
The ___ stain provides critical information about peptidoglycan structure of the cell wall. -
(ANSWER)Gram
Which of the following stains require heat fixation? - (ANSWER)Endospore
Gram
Simple
Acid-fast
Gram stain - (ANSWER)a stain that can differentiate between cells that have thick or thin layers of
peptidoglycan in their cell walls
Acid-Fast stain - (ANSWER)a stain that would be appropriate for cells that contain mycolic acid in their
cell walls.
Capsule stain - (ANSWER)a stain that enables you to effectively visualize a bacterium's glycocalyx
Which of the following provides a quick and effective method used to visualize and determine bacterial
morphology and arrangement? - (ANSWER)Simple stain
If you decolorize for more than 30 seconds, gram-positive organisms will most likely appear ____ in
color. - (ANSWER)pink
What might happen if you omitted the safranin step while performing the Gram stain on the mixed
culture of gram-positive and gram-negative cells? - (ANSWER)Gram-negative organisms might not be
visible
, Microbiology Lab Questions With Complete Solutions
if you omit the Gram's iodine step while preforming the Gram stain, gram-positive cells will most likely
be ____ in color. - (ANSWER)pink
What outcome could be expected if you forgot the crystal violet step while performing a Gram stain? -
(ANSWER)Gram-positive and gram-negative organisms would stain pink
Gram-negative cells appear __ after Gram staining whereas gram-positive cells appear __. -
(ANSWER)red, purple
If you forget to use the decolorizer, which cell type(s) will appear purple? - (ANSWER)Gram-positive and
Gram-negative
What is the primary dye used in the gram staining technique? - (ANSWER)crystal violet
What is used as the mordant in the gram staining technique? - (ANSWER)Gram's iodine
which of the following correctly describe how the Gram stain works to color different types of cells? -
(ANSWER)gram-positive cells retain the purple dye-mordant complex because of their thick layer of
peptidoglycan.
gram-negative cells are decolorized and stained by the pink/red secondary stain
which of the following describes the correct sequence of the gram stain procedure? - (ANSWER)heat-
fixed slide; use of crystal violet, iodine, decolorizer, and safranin
which of the following are terms used to describe the colored dyes applied in the Gram staining
technique? - (ANSWER)primary and counterstain
what is thought to cause gram-positive cells to retain the primary stain and the mordant complex
whereas gram-negative cells are decolorized? - (ANSWER)the thickness of the peptidoglycan layer
