MLS Review, Microbiology Questions With Complete
Solutions
> 20 mm Correct Answers An oxacillin disk screen test is used
to detect strep pneumo resistance to penicillin. Using mueller-
hinton agar with 5% sheep blood and a 1 ug oxacillin disk,what
is the recommended inhibition zone size for penicillin
susceptibility:
10 unit penicillin disk Correct Answers Nonpathogenic
moraxella spp. capable of growing on selective media for
neisseria can be differentaiated from Neisseria spp. by which
test?
4.5 Correct Answers at what pH does the methyl red test (MR)
become positive?
5 ug of novobiocin Correct Answers Staphylococcus
saprophyticus is best differentiated from staph. epidermidis by
resistance to:
5% O2, 10% CO2, and 85% N2 Correct Answers what
atmospheric condition is needed to recover campylobacter spp.
from specimens inoculated onto a campy-selective agar at 35-37
'C and 42'C?
60 sec Correct Answers when cleaning the skin with alcohol
then iodine for a blood culture collection, the iodine should
remain intact on the skin for at least:
,70% alcohol then 2% iodine (or an iodiphor) Correct Answers
the aseptic collection of blood cultures requires that the skin be
cleansed with:
acetoin Correct Answers the VP (voges-proskauer) test detects
which end product of glucose fermentation?
acetoin Correct Answers Which test should be used to
differentiate staph. aureus from staph intermedius?
acintobacillus Correct Answers Which genus of small gram-
negative coccobacilli is associated mainly with animals but may
cause endocarditis, bacteremia, as well as wound and dental
infections? (most species are oxidase and catalase positive)
amines Correct Answers decarboxylation of the amino acids
lysine, ornithine, and arginine results in the formation of:
aminoglycosides Correct Answers what class of antibiotics is
used for the treatment of serious gram-negative infections as
well as infections with Mycobacterium tuberculosis?
ammonium carbonate Correct Answers In the test for urease
production, ammonia reacts to form which product?
antibiotics and nutrients Correct Answers specimens for viral
culture should be transported in media containing:
b Correct Answers The majority of Haemophilus influenzae
infections are caused by which of the following capsular
serotypes?
, bacillus spp. Correct Answers Large gram positive sprore
forming rods growing on blood agar as large raised beta-
hemolytic colonies that spread and appear as frosted green/gray
glass are most likely:
bacteroides, peptostreptococcus, and clostridium spp. Correct
Answers prereduced and vitamin K1-supplemented blood agar
plates are recommended isolation media for:
bartonella spp. Correct Answers An elderly woman with several
cats was hospitalized with suspected cat scratch disease (CSD).
Blood cultures appeared negative, but a small, slightly curved
pleomorphic, gram negative rod grew on Brain heart infusion
agar with 5% horse or rabbit blood. What is the most likely
identification?
basic fuchsin and thionin Correct Answers In addition to CO2
requirements and biochemical characteristics, Brucella
melitensis and brucella abortus are differentiated by growth on
media containing which dyes?
biphasic castenada bottles with brucella broth Correct Answers
Which medium is recommended for the recovery of Brucela spp.
from blood and bone marrow specimens?
bordatella pertussis Correct Answers A 5 year old,
nonimmunized male with a persistent cough, fever, and flulike
symptoms was admitted. Nasopharyngeal swabs were cultured
on 15% blood, chocolate, bordet-genjou and regan lowe with
10% charcoal agars. All media grew gram negative
Solutions
> 20 mm Correct Answers An oxacillin disk screen test is used
to detect strep pneumo resistance to penicillin. Using mueller-
hinton agar with 5% sheep blood and a 1 ug oxacillin disk,what
is the recommended inhibition zone size for penicillin
susceptibility:
10 unit penicillin disk Correct Answers Nonpathogenic
moraxella spp. capable of growing on selective media for
neisseria can be differentaiated from Neisseria spp. by which
test?
4.5 Correct Answers at what pH does the methyl red test (MR)
become positive?
5 ug of novobiocin Correct Answers Staphylococcus
saprophyticus is best differentiated from staph. epidermidis by
resistance to:
5% O2, 10% CO2, and 85% N2 Correct Answers what
atmospheric condition is needed to recover campylobacter spp.
from specimens inoculated onto a campy-selective agar at 35-37
'C and 42'C?
60 sec Correct Answers when cleaning the skin with alcohol
then iodine for a blood culture collection, the iodine should
remain intact on the skin for at least:
,70% alcohol then 2% iodine (or an iodiphor) Correct Answers
the aseptic collection of blood cultures requires that the skin be
cleansed with:
acetoin Correct Answers the VP (voges-proskauer) test detects
which end product of glucose fermentation?
acetoin Correct Answers Which test should be used to
differentiate staph. aureus from staph intermedius?
acintobacillus Correct Answers Which genus of small gram-
negative coccobacilli is associated mainly with animals but may
cause endocarditis, bacteremia, as well as wound and dental
infections? (most species are oxidase and catalase positive)
amines Correct Answers decarboxylation of the amino acids
lysine, ornithine, and arginine results in the formation of:
aminoglycosides Correct Answers what class of antibiotics is
used for the treatment of serious gram-negative infections as
well as infections with Mycobacterium tuberculosis?
ammonium carbonate Correct Answers In the test for urease
production, ammonia reacts to form which product?
antibiotics and nutrients Correct Answers specimens for viral
culture should be transported in media containing:
b Correct Answers The majority of Haemophilus influenzae
infections are caused by which of the following capsular
serotypes?
, bacillus spp. Correct Answers Large gram positive sprore
forming rods growing on blood agar as large raised beta-
hemolytic colonies that spread and appear as frosted green/gray
glass are most likely:
bacteroides, peptostreptococcus, and clostridium spp. Correct
Answers prereduced and vitamin K1-supplemented blood agar
plates are recommended isolation media for:
bartonella spp. Correct Answers An elderly woman with several
cats was hospitalized with suspected cat scratch disease (CSD).
Blood cultures appeared negative, but a small, slightly curved
pleomorphic, gram negative rod grew on Brain heart infusion
agar with 5% horse or rabbit blood. What is the most likely
identification?
basic fuchsin and thionin Correct Answers In addition to CO2
requirements and biochemical characteristics, Brucella
melitensis and brucella abortus are differentiated by growth on
media containing which dyes?
biphasic castenada bottles with brucella broth Correct Answers
Which medium is recommended for the recovery of Brucela spp.
from blood and bone marrow specimens?
bordatella pertussis Correct Answers A 5 year old,
nonimmunized male with a persistent cough, fever, and flulike
symptoms was admitted. Nasopharyngeal swabs were cultured
on 15% blood, chocolate, bordet-genjou and regan lowe with
10% charcoal agars. All media grew gram negative
