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MLS ASCP Microbiology Exam 2025: Practice Questions, Study Guide & Review

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Prepare for the MLS ASCP Microbiology certification exam with this comprehensive 2025 resource. Includes realistic practice questions, detailed answer explanations, and a focused review of key topics like bacteriology, mycology, parasitology, virology, and laboratory procedures. Build confidence and pass your board exam on the first try.

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MLS ASCP Microbiology Actual Exam Newest
2025/2026 Complete All Questions And
Correct Detailed Answers (Verified Answers)
|Already Graded A+||Brand New Version!!


1 : 10 ratio - . . ANSWER ✔ ✔The proper blood-to-broth
ratio for blood cultures to reduce the antibacterial effects
of serum


Beta-lactamase - . . ANSWER ✔ ✔Produced by a
penicillin-resistant N. gonorrhoeae


Columbia CNA with 5% sheep blood - . . ANSWER ✔
✔Selective medium used for the isolation of gram-positive
microorgnisms


Indole test - . . ANSWER ✔ ✔used for determining an
organism's ability to produce indole from deamination of
tryptophan


JEMBEC system - . . ANSWER ✔ ✔is a transport and
inoculation medium used for direct plating of specimens

,2|Page


for N gonorrhoeae. Chocolate based selective medium is
inoculated with the specimen. This is placed in an
impermeable plastic bag with a bicarbonate-sodium citrate
pellet to generate a carbon dioxide rich environment.


Martin Lewis agar - . . ANSWER ✔ ✔Chocolate agar base
containing vancomycin, colistin, anisomycin, and
trimethoprim for better inhibition of gram-positive
organisms and candida.


Differential medium - . . ANSWER ✔ ✔aids in the
presumptive identification of organisms based on their
apperance


SPS - . . ANSWER ✔ ✔used as anticoagulant in blood
cultures to inactivate neutrophils and components of
serum complement


Blood culture - . . ANSWER ✔ ✔Typhoid fever can be
confirmed by this culture


Regan-Lowe agar - . . ANSWER ✔ ✔best medium for
culture for Bordetella pertussis

,3|Page




Francisella tularensis - . . ANSWER ✔ ✔most rapid
method for detection is PCR


synovial infections - . . ANSWER ✔ ✔Anaerobic
susceptibility testing is helpful


Schlichter test - . . ANSWER ✔ ✔is used to monitor
bactericidal activity during antimicrobic therapy in cases
of endocaditis


Moraxella osloensis - . . ANSWER ✔ ✔is a gram-negative
coccobacillus that is often plump and occurs in pairs and
demonstrates a morphology similar to Neisseria


Extra-intestinal isolates of Salmonella - . . ANSWER ✔
✔should be tested for resistance to nalidixic acid and if
resistant report the ciprofloxacin as resistant


Gentamicin - . . ANSWER ✔ ✔antimicrobial resistance
occurs from enzymatic drug modification

, 4|Page


Enterococcus infection - . . ANSWER ✔ ✔need 2
antimicrobial agents, penicillin derivative and an
aminoglycoside


pulsed-field gel electrophoresis - . . ANSWER ✔
✔commonly used method to determine the relatedness of
2 or more bacterial strains


Micrococcus - . . ANSWER ✔ ✔is modified oxidase
positive, bacitracin (0.04U) susceptible and resistant to
lysotaphin


Enterococcus - . . ANSWER ✔ ✔may be used as a positive
quality control for the bile esculin test


Campylobacter - . . ANSWER ✔ ✔are gram-negative,
curved bacilli "gull wings" that require microaerophilic
conditions for growth


Porphyrin test - . . ANSWER ✔ ✔was devised to detect
strains of Haemophilus capable of synthesis of hemin

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