Molecular Diagnostics Fundamentals,
Methods and Clinical Applications
with Complete Solutions
(AMR)The range within which a specimen may be measured directly (without dilution of
concentration) for an analyte - ANSWER-Analyte measurement range (AMR)
a 12-kD peptide associated at the cell membrane with the MHC heavy chain comprising
the class 1 HLA - ANSWER-Beta 2 microglobulin
a complex of proteins factors required by RNA polymerase to initiate RNA synthesis -
ANSWER-basal transcription complex
a cyclic protein found in antibiotics - ANSWER-Beta-lactam
a different version of the same sequence, gene or locus - ANSWER-allele
A DNA sequence difference, the phenotypic effect of which is counteracted by a second
trait or polymorphism - ANSWER-balanced polymorphism
a PCR method for amplification of complex targets using random priming with 6- to 10-
base primers for typing of organisms; also known as randomly amplified polymorphic
DNA (RAPD) - ANSWER-Arbitrarily Primed PCR
A position on the DNA sugar-phosphate backbone where the ribose sugar does not
carry a nitrogen base - ANSWER-abasic site
a reaction mix containing all components except for target; used to detect target
contamination in test reagents - ANSWER-blank
a set of PCR amplicons of a VNTR or STR that represents all possible alleles in a
population - ANSWER-allelic ladder
a structure visible in the interphase nucleus formed by the inactive X chromosome in
female mammals - ANSWER-Barr body
a synthetic short RNA that antagonizes oncomirs through complementary hybridization.
- ANSWER-antimir
a transplanted organ from a genetically different donor - ANSWER-allograft
A virus that infects bacteria - ANSWER-bacteriophage
, a vitamin that is used in the laboratory for nonradioactive probe detection - ANSWER-
Biotin
ability of a method to detect only the analyte and not nonspecific targets - ANSWER-
Analytic specificity
adjacent site; location of incoming charged tRNA binding in the ribosome complex for
acceptance of the growing peptide - ANSWER-A site
AFLP; analysis of polymorphic DNA by amplification of selected regions and resolving
the amplified products by size - ANSWER-Amplified Fragment Length Polymorphism
(AFLP)
AHA; alloantibodies; antibodies that recognize human antigens - ANSWER-anti-human
antibodies
an adaptation of information technology for the biological sciences; computational
biology - ANSWER-Bioinformatics
an enzyme frequently used to generate signals from chemiluminescent or chromogenic
substrates. - ANSWER-Alkaline phosphatase
an enzyme produced by bacteria that are resistant to β-lactam-containing antibiotics -
ANSWER-Beta-lactamase
an expression of units of double-stranded nucleic acid - ANSWER-base pair
an image produced by exposing a light-sensitive film to a light- or radiation-emitting
membrane - ANSWER-Autoradiograph
an inheritance pattern where the child of an affected and an unaffected individual has a
50% probability of being affected - ANSWER-autosomal dominant
an inheritance pattern where the child of an affected and an unaffected individual has a
50% probability of being affected and where the child of two affected individuals has a
100% probability of being affected - ANSWER-autosomal recessive
an ionic substance or solution that maintains specific pH - ANSWER-buffer
antibiotic; a substance that inhibits growth of or kills bacteria - ANSWER-Antimicrobial
agent
antibodies that recognize human antigens - ANSWER-alloantibodies
Any chromosome that is not a X or Y chromosome - ANSWER-autosome
Methods and Clinical Applications
with Complete Solutions
(AMR)The range within which a specimen may be measured directly (without dilution of
concentration) for an analyte - ANSWER-Analyte measurement range (AMR)
a 12-kD peptide associated at the cell membrane with the MHC heavy chain comprising
the class 1 HLA - ANSWER-Beta 2 microglobulin
a complex of proteins factors required by RNA polymerase to initiate RNA synthesis -
ANSWER-basal transcription complex
a cyclic protein found in antibiotics - ANSWER-Beta-lactam
a different version of the same sequence, gene or locus - ANSWER-allele
A DNA sequence difference, the phenotypic effect of which is counteracted by a second
trait or polymorphism - ANSWER-balanced polymorphism
a PCR method for amplification of complex targets using random priming with 6- to 10-
base primers for typing of organisms; also known as randomly amplified polymorphic
DNA (RAPD) - ANSWER-Arbitrarily Primed PCR
A position on the DNA sugar-phosphate backbone where the ribose sugar does not
carry a nitrogen base - ANSWER-abasic site
a reaction mix containing all components except for target; used to detect target
contamination in test reagents - ANSWER-blank
a set of PCR amplicons of a VNTR or STR that represents all possible alleles in a
population - ANSWER-allelic ladder
a structure visible in the interphase nucleus formed by the inactive X chromosome in
female mammals - ANSWER-Barr body
a synthetic short RNA that antagonizes oncomirs through complementary hybridization.
- ANSWER-antimir
a transplanted organ from a genetically different donor - ANSWER-allograft
A virus that infects bacteria - ANSWER-bacteriophage
, a vitamin that is used in the laboratory for nonradioactive probe detection - ANSWER-
Biotin
ability of a method to detect only the analyte and not nonspecific targets - ANSWER-
Analytic specificity
adjacent site; location of incoming charged tRNA binding in the ribosome complex for
acceptance of the growing peptide - ANSWER-A site
AFLP; analysis of polymorphic DNA by amplification of selected regions and resolving
the amplified products by size - ANSWER-Amplified Fragment Length Polymorphism
(AFLP)
AHA; alloantibodies; antibodies that recognize human antigens - ANSWER-anti-human
antibodies
an adaptation of information technology for the biological sciences; computational
biology - ANSWER-Bioinformatics
an enzyme frequently used to generate signals from chemiluminescent or chromogenic
substrates. - ANSWER-Alkaline phosphatase
an enzyme produced by bacteria that are resistant to β-lactam-containing antibiotics -
ANSWER-Beta-lactamase
an expression of units of double-stranded nucleic acid - ANSWER-base pair
an image produced by exposing a light-sensitive film to a light- or radiation-emitting
membrane - ANSWER-Autoradiograph
an inheritance pattern where the child of an affected and an unaffected individual has a
50% probability of being affected - ANSWER-autosomal dominant
an inheritance pattern where the child of an affected and an unaffected individual has a
50% probability of being affected and where the child of two affected individuals has a
100% probability of being affected - ANSWER-autosomal recessive
an ionic substance or solution that maintains specific pH - ANSWER-buffer
antibiotic; a substance that inhibits growth of or kills bacteria - ANSWER-Antimicrobial
agent
antibodies that recognize human antigens - ANSWER-alloantibodies
Any chromosome that is not a X or Y chromosome - ANSWER-autosome
