Practice Exam 1
Biochemistry I (SUNY Old
Westbury)
, CP4510 Practice Exam 1 Fall 2019
1. What functional groups are present on this molecule?
A) ether and aldehyde
B) hydroxyl and aldehyde
C) hydroxyl and carboxylic acid
D) hydroxyl and ester
E) hydroxyl and ketone
2. If the free energy change ΔG for a reaction is -46.11 kJ/mol, the reaction is:
A) at equilibrium. B) endergonic. C) endothermic. D) exergonic. E) exothermic.
3. Consider an acetate buffer, initially at the same pH as its pKa (4.76). When sodium
hydroxide (NaOH) is mixed with this buffer, the:
A) pH remains constant.
B) pH rises more than if an equal amount of NaOH is added to unbuffered water at pH 4.76.
C) ratio of sodium acetate to acetic acid in the buffer increases.
D) sodium acetate formed precipitates because it is less soluble than acetic acid.
4. Phosphoric acid is tribasic, with pKa’s of 2.14, 6.86, and 12.4. The ionic form that
predominates at pH
3.2 is:
A) H3PO4. B) H2PO4–. C) HPO42–. D) PO43–. E) None of the above.
5. The three-dimensional structure of a protein is determined primarily by:
A) electrostatic guidance from nucleic acid structure.
B) how many amino acids are in the protein.
C) hydrophobic interaction with lipids that provide a folding framework.
D) modification during interactions with ribosomes.
E) the sequence of amino acids in the protein.
6. All of the amino acids that are found in proteins, except for proline, contain a(n):
A) amino group. B) carbonyl group C) carboxyl group D) ester group
E) thiol group.
7. In a mixture of the five proteins listed below, which should elute second in size-
exclusion (gel- filtration) chromatography?
A) cytochrome c Mr = 13,00
0
B) immunoglobulin Mr = 145,00
G 0
C) ribonuclease A Mr = 13,70
0
D) RNA polymerase Mr = 450,00
0
E) serum albumin Mr = 68,50
0
Biochemistry I (SUNY Old
Westbury)
, CP4510 Practice Exam 1 Fall 2019
1. What functional groups are present on this molecule?
A) ether and aldehyde
B) hydroxyl and aldehyde
C) hydroxyl and carboxylic acid
D) hydroxyl and ester
E) hydroxyl and ketone
2. If the free energy change ΔG for a reaction is -46.11 kJ/mol, the reaction is:
A) at equilibrium. B) endergonic. C) endothermic. D) exergonic. E) exothermic.
3. Consider an acetate buffer, initially at the same pH as its pKa (4.76). When sodium
hydroxide (NaOH) is mixed with this buffer, the:
A) pH remains constant.
B) pH rises more than if an equal amount of NaOH is added to unbuffered water at pH 4.76.
C) ratio of sodium acetate to acetic acid in the buffer increases.
D) sodium acetate formed precipitates because it is less soluble than acetic acid.
4. Phosphoric acid is tribasic, with pKa’s of 2.14, 6.86, and 12.4. The ionic form that
predominates at pH
3.2 is:
A) H3PO4. B) H2PO4–. C) HPO42–. D) PO43–. E) None of the above.
5. The three-dimensional structure of a protein is determined primarily by:
A) electrostatic guidance from nucleic acid structure.
B) how many amino acids are in the protein.
C) hydrophobic interaction with lipids that provide a folding framework.
D) modification during interactions with ribosomes.
E) the sequence of amino acids in the protein.
6. All of the amino acids that are found in proteins, except for proline, contain a(n):
A) amino group. B) carbonyl group C) carboxyl group D) ester group
E) thiol group.
7. In a mixture of the five proteins listed below, which should elute second in size-
exclusion (gel- filtration) chromatography?
A) cytochrome c Mr = 13,00
0
B) immunoglobulin Mr = 145,00
G 0
C) ribonuclease A Mr = 13,70
0
D) RNA polymerase Mr = 450,00
0
E) serum albumin Mr = 68,50
0
