BIOTECH BACE CORE EXAMS SET UPDATED QUESTIONS
AND ANSWERS GRADED A+
✔✔A technician is asked to verify the presence of a protein of interest after a production
run. Following purification, a sample is run on an SDS-PAGE gel, and a band appears
at the expected molecular weight. Which of the following techniques is the best method
to confirm that the band represents the purified protein of interest? - ✔✔Run a western
blot with a sample of the fraction
✔✔Which of the following best describes the basic principle of an ELISA? -
✔✔Detection of an antigen-antibody interaction using an enzyme-conjugated antibody
✔✔Diagnostic immunoassays for infectious diseases, such as strep throat, are
commonly used by physicians to quickly diagnose patients. These immunoassays utilize
the reaction of antibodies to which of the following from a patient's sample? -
✔✔Antigens from the pathogen
✔✔Which of the following molecular diagnostic techniques can be used to detect
specific DNA sequences associated with genetic disorders? - ✔✔Polymerase Chain
Reaction (PCR)
✔✔Which of the following parameters is NOT typically evaluated during the validation of
an assay? - ✔✔System Compatibility
✔✔What is the primary function of fluorescence-activated cell sorting (FACS)? - ✔✔To
separate and sort a population of cells based on their fluorescence properties
✔✔Which of the following best describes the principle of centrifugation? - ✔✔Use of
gravitational force to separate particles in a solution based on their size, shape, and
density
✔✔Why is plasmid DNA purified using anion exchange column chromatography? -
✔✔DNA is negatively charged, binds to the positively charged resin, and is eluted with
increasing salt.
✔✔Which of the following best describes the principle of chromatography? -
✔✔Separation of components in a mixture via interactions with stationary and mobile
phases
✔✔Gel-Filtration/Size Exclusion Chromatography (SEC) - ✔✔Separates proteins based
on size
✔✔Ion Exchange Chromatography (IEC) - ✔✔Separates proteins based on charge
, ✔✔Affinity Chromatography - ✔✔Separates proteins based on unique functional groups
✔✔Hydrophobic Interaction Chromatography (HIC) - ✔✔Separates proteins based on
solubility in water
✔✔When performing a transformation, the cold calcium chloride solution makes the
cells chemically competent. Which of following is the primary feature of chemically
competent cells? - ✔✔They are more permeable to DNA.
✔✔Which of the following is a primary benefit of using automation and robotics in
biotechnology laboratories? - ✔✔Increased throughput and consistency in performing
repetitive tasks
✔✔Preventive maintenance (PM) and calibration on laboratory equipment is scheduled
according to which of the following? - ✔✔Based on the company's SOPs for PM and
calibration, Based on how often the equipment is used, Based on the equipment
manufacturer's suggestions.
✔✔Laminar Flow Hood - ✔✔A device that provides a sterile, unidirectional flow of
filtered air over a work surface.
✔✔HEPA Filter - ✔✔A filter that removes at least 99.97% of airborne particles larger
than 0.3 micrometers.
✔✔ISO Class 5 Cleanroom - ✔✔An environment with fewer than 3,520 particles per
cubic meter of air.
✔✔Aseptic Processing - ✔✔The process of filling and packaging products without
contamination.
✔✔Turbulence - ✔✔Disruption in airflow that can cause contamination in a controlled
environment.
✔✔"Bioethics" is best described as: - ✔✔The study of decision making as it applies to
moral decisions that must be made due to advances in biology and technology.
✔✔Working for Company A, a technician was trained on a particular method for
performing an assay. Now working at Company B, the technician is asked to perform
the same assay using Company B's test record. The technician notes that Company B's
method is much slower. Which of the following is the correct course of action? -
✔✔Follow the Company B test record, even though Company A's method is faster
✔✔Which of the following is permitted in the laboratory? - ✔✔Eye glasses
AND ANSWERS GRADED A+
✔✔A technician is asked to verify the presence of a protein of interest after a production
run. Following purification, a sample is run on an SDS-PAGE gel, and a band appears
at the expected molecular weight. Which of the following techniques is the best method
to confirm that the band represents the purified protein of interest? - ✔✔Run a western
blot with a sample of the fraction
✔✔Which of the following best describes the basic principle of an ELISA? -
✔✔Detection of an antigen-antibody interaction using an enzyme-conjugated antibody
✔✔Diagnostic immunoassays for infectious diseases, such as strep throat, are
commonly used by physicians to quickly diagnose patients. These immunoassays utilize
the reaction of antibodies to which of the following from a patient's sample? -
✔✔Antigens from the pathogen
✔✔Which of the following molecular diagnostic techniques can be used to detect
specific DNA sequences associated with genetic disorders? - ✔✔Polymerase Chain
Reaction (PCR)
✔✔Which of the following parameters is NOT typically evaluated during the validation of
an assay? - ✔✔System Compatibility
✔✔What is the primary function of fluorescence-activated cell sorting (FACS)? - ✔✔To
separate and sort a population of cells based on their fluorescence properties
✔✔Which of the following best describes the principle of centrifugation? - ✔✔Use of
gravitational force to separate particles in a solution based on their size, shape, and
density
✔✔Why is plasmid DNA purified using anion exchange column chromatography? -
✔✔DNA is negatively charged, binds to the positively charged resin, and is eluted with
increasing salt.
✔✔Which of the following best describes the principle of chromatography? -
✔✔Separation of components in a mixture via interactions with stationary and mobile
phases
✔✔Gel-Filtration/Size Exclusion Chromatography (SEC) - ✔✔Separates proteins based
on size
✔✔Ion Exchange Chromatography (IEC) - ✔✔Separates proteins based on charge
, ✔✔Affinity Chromatography - ✔✔Separates proteins based on unique functional groups
✔✔Hydrophobic Interaction Chromatography (HIC) - ✔✔Separates proteins based on
solubility in water
✔✔When performing a transformation, the cold calcium chloride solution makes the
cells chemically competent. Which of following is the primary feature of chemically
competent cells? - ✔✔They are more permeable to DNA.
✔✔Which of the following is a primary benefit of using automation and robotics in
biotechnology laboratories? - ✔✔Increased throughput and consistency in performing
repetitive tasks
✔✔Preventive maintenance (PM) and calibration on laboratory equipment is scheduled
according to which of the following? - ✔✔Based on the company's SOPs for PM and
calibration, Based on how often the equipment is used, Based on the equipment
manufacturer's suggestions.
✔✔Laminar Flow Hood - ✔✔A device that provides a sterile, unidirectional flow of
filtered air over a work surface.
✔✔HEPA Filter - ✔✔A filter that removes at least 99.97% of airborne particles larger
than 0.3 micrometers.
✔✔ISO Class 5 Cleanroom - ✔✔An environment with fewer than 3,520 particles per
cubic meter of air.
✔✔Aseptic Processing - ✔✔The process of filling and packaging products without
contamination.
✔✔Turbulence - ✔✔Disruption in airflow that can cause contamination in a controlled
environment.
✔✔"Bioethics" is best described as: - ✔✔The study of decision making as it applies to
moral decisions that must be made due to advances in biology and technology.
✔✔Working for Company A, a technician was trained on a particular method for
performing an assay. Now working at Company B, the technician is asked to perform
the same assay using Company B's test record. The technician notes that Company B's
method is much slower. Which of the following is the correct course of action? -
✔✔Follow the Company B test record, even though Company A's method is faster
✔✔Which of the following is permitted in the laboratory? - ✔✔Eye glasses
