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Samenvatting Plasma 1 voorbereiding bio-analytisch practicum

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Dit document omvat alle voorbereidende info omtrent het practicum 'plasma 1' van het vak Bio-analytisch practicum. Dit is te kennen leerstof voor het examen en is te kennen voor eventuele ondervragingen.

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Universiteit Gent (UGent)
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Bio analytisch practicum (J000506A)








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Institution
Universiteit Gent (UGent)
Study
Farmaceutische Wetenschappen
Course
Bio analytisch practicum (J000506A)
All documents for this subject (19)

Document information

Uploaded on
October 30, 2025
Number of pages
4
Written in
2025/2026
Type
Summary

Subjects

  • voorbereiding
  • plasma 1
  • bio analytisch practicum
  • samenvatting

Content preview

 Plasma 1: Bepaling van glucose in plasma (colorimetrisch)


Principe:

Glucose bepaling in plasma via de Somogyi-Nelson methode

Proteïne precipitatie + colorimetrische reactie

Kwantificatie: UV-spectrofotometrie



Relevantie:

- Glucose ~ stofwisseling
- “verouderde” methode (~1950)
- Proteïne precipitatie
- Vergelijking andere glucose kwantificatie methode (cfr. Plasma 2)



Proteïne precipitatie – staalopzuivering:

- Zinksulfaat

• precipitatie proteïnen

• precipitatie -SH componenten

- Barium-hydroxide

• + Zinksulfaat  Barium-sulfaat

• Barium-sulfaat  precipitatie urinezuur, creatinine



Colorimetrische reactie:

- Koper reagens  oxidatie glucose

• Reductie koper (Cu+2  Cu2O)

- Arsenomolybdaat reagens  kleuring

• Oxidatie koper (Cu2O  Cu +2 )

• Reductie arsenomolybdaat = kleuring




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