2026 ACS Biochemistry Exam – Fully Updated
Questions with Verified Correct Answers (A+
Rated) Chamberlain University |
1. Henderson-Hasselbach Equation --solution-- pH = pKa + log ([A-] / [HA])
2. FMOC Chemical Synthesis --solution-- Used in synthesis of a growing amino acid
chain to a polystyrene bead. FMOC is used as a protecting group on the N- terminus.
3. Salting Out (Purification) --solution-- Changes soluble protein to solid precipitate.
Protein precipitates when the charges on the protein match the charges in the solution.
4. Size-Exclusion Chromatography --solution-- Separates sample based on size with
smaller molecules eluting later.
5. Ion-Exchange Chromatography --solution-- Separates sample based on charge. CM
attracts +, DEAE attracts -. May have repulsion effect on like charges. Salt or acid used
to remove stuck proteins.
6. Hydrophobic/Reverse Phase Chromatography --solution-- Beads are coated with a
carbon chain. Hydrophobic proteins stick better. Elute with non-H-bonding solvent
(acetonitrile).
Questions with Verified Correct Answers (A+
Rated) Chamberlain University |
1. Henderson-Hasselbach Equation --solution-- pH = pKa + log ([A-] / [HA])
2. FMOC Chemical Synthesis --solution-- Used in synthesis of a growing amino acid
chain to a polystyrene bead. FMOC is used as a protecting group on the N- terminus.
3. Salting Out (Purification) --solution-- Changes soluble protein to solid precipitate.
Protein precipitates when the charges on the protein match the charges in the solution.
4. Size-Exclusion Chromatography --solution-- Separates sample based on size with
smaller molecules eluting later.
5. Ion-Exchange Chromatography --solution-- Separates sample based on charge. CM
attracts +, DEAE attracts -. May have repulsion effect on like charges. Salt or acid used
to remove stuck proteins.
6. Hydrophobic/Reverse Phase Chromatography --solution-- Beads are coated with a
carbon chain. Hydrophobic proteins stick better. Elute with non-H-bonding solvent
(acetonitrile).
