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QMUL BIO163 (Molecular Genetics) WHOLE MODULE NOTES/SUMMARY

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Extensive notes for Molecular Genetics module. Full notes, revision for exams etc. I study medical genetics, got a first in my first year!

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DNA ,
RNA & Proteins
Central
Dogma Theory that genetic information flows only in one
z



direction : DNA RNA Protein or RNA- Protein

-

However ,
in some cases RNA can be Wanscibed
reverse to DNA



Nucleotide structure

Each monomer of DNA is a nucleotide
-




Sugar phosphate group &nitogeneous
consists :
of
-



, ,




base

RNA by2,
o

inters
Deoxyribose in DNA nbose in
-


,




Polymer molecules

Nucleotides
join together in condensation reactions
-




,




forming a
hydrogen bond and releasing a molecule
of water
per condensation reaction



Vanities of nucleotides
-

structres of nucleotides determines

their
hydrogen bonding potential
Pyrmidines bond with
purines
-




Forming a heux
* strands
negative charges the phosphate backbone
repel
-




on
are anti
each other , the bases stack inside the helix -

parallel
structure with equal spacing . To
between them

fit bases and sugars , the backbone spirals ,
creating
a
major and minor
groove . CG bonds stronger asthey

bonds
have 3
hydrogen

, DNA vs RNA
RNA uses nibose
sugar
-




DNA stable tran RNA CRNA
more
degrades quicker)
-




reacility and decreases
The 2 ots that RNA has increases
stability
-




RNA Uracil instead of thymine important for DNA
uses repair
-




,




mechanisms

-DNA is its stable
a better
storage molecule as more , it can be


packaged and its
longer

Protein shuchwe
-

10- order of amino acids
. Has peptide bonds
-
27 alpha helix/beta pleated sheets. Has

hydrogen bonds

37 further .
folding Has
hydrogen bonds,
=




disulfide and ionic bonds
bridges
4- shuctves created from multiple
polypeptide chains
-




A DNA , RNA and proteins are directional molecules , one end is different
than the other end




Types of RNA
-

MRNA
-
tRNA
-

SiRNA
-

MiRNA
-


rRNA


regulatory RNAs
-

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