June 2025 Edexcel: A Level Biology B 9BI0/01 Paper 1 – Advanced Biochemistry, Microbiology and Genetics – Merged Question Paper & Mark Scheme

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Number Number June 2025 Edexcel: A Level Biology B
9BI0/01 Paper 1 – Advanced
Biochemistry, Microbiology and
Genetics – Merged Question Paper &
Mark Scheme




Paper


B 🟐 🟐


Advanced
PAPER 1: Advanced Biochemistry, Microbiology
and Genetics




Instructions

•• Use black ink or ball-point pen.
Fill in the boxes at the top of this page with your name,
centre number and candidate number.
•• Answer all questions.
Answer the questions in the spaces provided
– there may be more space than you need.

Information

•• The total mark for this paper is 90.
The marks for each question are shown in brackets

•Microbiology
– useResource
Exam this as a guide as to how much time to spend on each question.
Summary
TheIn question(s)
June 2025 marked
Edexcel Awith
Level asteriskB (*),
anBiology marks– will
9BI0/01 Paperbe1:awarded for Biochemistry,
Advanced your ability
Genetics –
to structure your answer logically showing how the points that you make are
and Merged Question Paper & Mark Scheme document integrates
therelated
official or follow on from
examination papereach
with other where mark
its complete appropriate.
scheme. This paper assesses students’
understanding of advanced biochemical processes, microbiological principles, and genetic
Advice
mechanisms, including molecular structures, enzyme activity, microbial growth and control,

•
DNA replication, and inheritance patterns. The merged format presents each question
Read each questioncriteria,
carefully before you startontoexaminer
answer it.
•
alongside
Try to
its marking
answer every
providing
question.
clarity expectations, mark allocation,

•
and question structure. This resource supports targeted revision, application of theoretical
Check your
knowledge answers if youand
to experimental have time at contexts,
real-world the end. and the development of analytical and
evaluative skills, making it essential for preparation for the June 2025 Edexcel A Level
Biology B Paper 1 examination.
Turn over


P74459A
©2025 Pearson Education Ltd.
Y:1/1/1/1/1/1/1/1/

, Answer ALL questions.

Some questions must be answered with a cross in a box . If you change your mind about an




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answer, put a line through the box and then mark your new answer with a cross .
1 Active transport is one type of cell transport mechanism.

(a) What happens during active transport?
(1)
A ATP is hydrolysed releasing energy

B ATP is hydrolysed using energy
C ATP is phosphorylated releasing energy

D ATP is phosphorylated using energy


(b) Which substance is transported against a concentration gradient by active
transport?
(1)




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A hydrophilic molecule

B large hydrophobic molecule
C small hydrophobic molecule

D water




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, (c) The graph shows the change in concentration of molecule A and molecule B, as
they are transported into the cytoplasm of a cell.
molecule A
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Concentration of molecules
in the cytoplasm molecule B




0 10 20 30
Time / mins

Explain the differences in the changes in concentration of molecule A and
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molecule B between 0 and 10 minutes and between 20 and 30 minutes.
(2)

. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . ......................



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. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . ......................



. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . ......................



(Total for Question 1 = 4 marks)
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3

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, 2 The diagram shows part of cellular respiration.

glucose




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glycolysis


molecule X
link reaction
and
Krebs cycle
carbon dioxide

(a) Which is molecule X?
(1)

A glycerate 3-phosphate
B glycogen




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C lactate

D pyruvate


(b) Which row of the table shows the locations of glycolysis, the link reaction and the
Krebs cycle in eukaryotic cells?
(1)

Glycolysis Link reaction Krebs cycle

A cytoplasm cytoplasm cytoplasm

B cytoplasm mitochondria mitochondria

C mitochondria cytoplasm cytoplasm

D mitochondria mitochondria mitochondria DO NOT WRITE IN THIS AREA




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