1
For Expert help and assignment solutions, +254707240657
Microbiology Lab Final Questions and
Answers (100% Correct Answers) Already
Graded A+
What type of organism is Elodea? [ Ans: ] plant
In protein synthesis, adenine pairs with ________________________,
and guanine pairs with ________________________. [ Ans: ] uracil,
cytosine
© 2025 Assignment Expert
A substance used to support the growth of microbial life is referred
to as a ___________________________. [ Ans: ] culture medium
A plasmid that is used to introduce a new "foreign" gene into a
Guru01 - Stuvia
recipient cell is referred to as _____________________. [ Ans: ] a
cloning vector
Although Staphylococcus aureus demonstrates optimal growth in
an environment of 1% salt, it is able to tolerate up to 13% salt. S.
aureus would be described as: [ Ans: ] halotolerant
Which of the following infectious agents is least likely to be
damaged by UV exposure? [ Ans: ] prions
A chemical compound, regardless of its source (either natural or
synthetic), that is capable of killing or inhibiting microorganisms is
referred to as: [ Ans: ] an antimicrobial
What biosafety levels do most introductory microbiology students
work with? [ Ans: ] BSL-1
You have inoculated a tube of SIM (sulfide indole motility) medium
and after incubation, the medium remained clear and you can
only see growth in the inoculation stab. What does this indicate? [
Ans: ] The organism is nonmotile.
What is the name of the reagent used for the oxidase test? [ Ans:
] tetramethyl-p-phenylenediamine
, 2
For Expert help and assignment solutions, +254707240657
The substrate binds at the enzyme's [ Ans: ] active site.
All living things are made of _____. [ Ans: ] cells
________ can be used to separate nucleic acids based on size. [
Ans: ] Gel electrophoresis
DNA can be collected on a glass rod in a technique called [ Ans:
] spooling
Vibrio cholerae is the bacterium responsible for cholera, a GI tract
infection. Normally, it takes the ingestion of over one million
bacterial cells to cause infection, but the consumption of antacids
signficantly lowers this number. What does this tell you about the
likely classification of V. cholerae in terms of pH preference? [
© 2025 Assignment Expert
Ans: ] It is likely an alkaliphile.
The structure that easily distinguishes a plant cell from an animal
cell is [ Ans: ] chloroplasts
Guru01 - Stuvia
A bacterial culture is being tested for its ability to reduce nitrate.
After incubation and growth of the bacteria in nitrate broth, there
is no accumulation of gas in the Durham tube. The test for the
presence of nitrite is colorless. When zinc powder is added, a
bright red color develops. Interpret these results. [ Ans: ] The
bacteria were unable to reduce nitrate.
Microorganisms that can grow at salt concentrations as high as 15
- 30% are known as: [ Ans: ] halophiles
Select the statement that is true about DNA profiling. [ Ans: ]
DNA profiling is the analysis of the collection of fragments
produced by cutting DNA with specific restriction endonucleases.
Why is antimicrobic sensitivity testing done? [ Ans: ] To determine
what antimicrobials would be most effective for treating the
infection
The approximate size of DNA fragment can be estimated on a gel
using the: [ Ans: ] DNA ladder
When you boil egg white (albumin is protein) you have [ Ans: ]
denatured the protein.
, 3
For Expert help and assignment solutions, +254707240657
A yellow slant and butt in Kligler's iron agar indicates [ Ans: ] both
glucose and lactose were fermented.
If you transfer bacterial growth from a broth to an agar plate but
nothing grows after incubation, what may have occurred? [ Ans:
] The loop may have been too hot when making the transfer.
Why is bromophenol blue added to the individual DNA samples? [
Ans: ] It allows the observer to view how far the DNA samples
travel.
If an organism growing on Kligler's iron agar ferments glucose, but
NOT lactose, why does the slant ultimately turn red? [ Ans: ]
When the glucose supply is exhausted, the organism turns to
© 2025 Assignment Expert
peptone degradation, releasing alkaline end products.
Which of the following would be appropriately sterilized using UV
radiation? [ Ans: ] stainless steel surface in biological safety
cabinet used for media preparation
Guru01 - Stuvia
Which of the following DNA sequences would be the most likely to
be damaged by UV radiation? [ Ans: ] CTTGAATTAA
Which of the following can determine the particular fermentation
pathway used by a fermenting microorganism? [ Ans: ]
Inoculation of MRVP broth, followed by MR and VP tests
As the concentration of an enzyme increases for a given
substrate, [ Ans: ] the reaction rate increases similarly.
What would you predict would happen to the fermentation rate if
you used yeast suspension that had not been swirled/mixed each
time before it was dispensed into test tubes? [ Ans: ] The reaction
rate would be very low.
Mitochondria are associated with the production of _____. [ Ans:
] ATP
Lab coats, gloves, face masks, and goggles are all examples of [
Ans: ] personal protective equipment
Which of the following substrates is fermented most easily by
yeast? [ Ans: ] glucose
For Expert help and assignment solutions, +254707240657
Microbiology Lab Final Questions and
Answers (100% Correct Answers) Already
Graded A+
What type of organism is Elodea? [ Ans: ] plant
In protein synthesis, adenine pairs with ________________________,
and guanine pairs with ________________________. [ Ans: ] uracil,
cytosine
© 2025 Assignment Expert
A substance used to support the growth of microbial life is referred
to as a ___________________________. [ Ans: ] culture medium
A plasmid that is used to introduce a new "foreign" gene into a
Guru01 - Stuvia
recipient cell is referred to as _____________________. [ Ans: ] a
cloning vector
Although Staphylococcus aureus demonstrates optimal growth in
an environment of 1% salt, it is able to tolerate up to 13% salt. S.
aureus would be described as: [ Ans: ] halotolerant
Which of the following infectious agents is least likely to be
damaged by UV exposure? [ Ans: ] prions
A chemical compound, regardless of its source (either natural or
synthetic), that is capable of killing or inhibiting microorganisms is
referred to as: [ Ans: ] an antimicrobial
What biosafety levels do most introductory microbiology students
work with? [ Ans: ] BSL-1
You have inoculated a tube of SIM (sulfide indole motility) medium
and after incubation, the medium remained clear and you can
only see growth in the inoculation stab. What does this indicate? [
Ans: ] The organism is nonmotile.
What is the name of the reagent used for the oxidase test? [ Ans:
] tetramethyl-p-phenylenediamine
, 2
For Expert help and assignment solutions, +254707240657
The substrate binds at the enzyme's [ Ans: ] active site.
All living things are made of _____. [ Ans: ] cells
________ can be used to separate nucleic acids based on size. [
Ans: ] Gel electrophoresis
DNA can be collected on a glass rod in a technique called [ Ans:
] spooling
Vibrio cholerae is the bacterium responsible for cholera, a GI tract
infection. Normally, it takes the ingestion of over one million
bacterial cells to cause infection, but the consumption of antacids
signficantly lowers this number. What does this tell you about the
likely classification of V. cholerae in terms of pH preference? [
© 2025 Assignment Expert
Ans: ] It is likely an alkaliphile.
The structure that easily distinguishes a plant cell from an animal
cell is [ Ans: ] chloroplasts
Guru01 - Stuvia
A bacterial culture is being tested for its ability to reduce nitrate.
After incubation and growth of the bacteria in nitrate broth, there
is no accumulation of gas in the Durham tube. The test for the
presence of nitrite is colorless. When zinc powder is added, a
bright red color develops. Interpret these results. [ Ans: ] The
bacteria were unable to reduce nitrate.
Microorganisms that can grow at salt concentrations as high as 15
- 30% are known as: [ Ans: ] halophiles
Select the statement that is true about DNA profiling. [ Ans: ]
DNA profiling is the analysis of the collection of fragments
produced by cutting DNA with specific restriction endonucleases.
Why is antimicrobic sensitivity testing done? [ Ans: ] To determine
what antimicrobials would be most effective for treating the
infection
The approximate size of DNA fragment can be estimated on a gel
using the: [ Ans: ] DNA ladder
When you boil egg white (albumin is protein) you have [ Ans: ]
denatured the protein.
, 3
For Expert help and assignment solutions, +254707240657
A yellow slant and butt in Kligler's iron agar indicates [ Ans: ] both
glucose and lactose were fermented.
If you transfer bacterial growth from a broth to an agar plate but
nothing grows after incubation, what may have occurred? [ Ans:
] The loop may have been too hot when making the transfer.
Why is bromophenol blue added to the individual DNA samples? [
Ans: ] It allows the observer to view how far the DNA samples
travel.
If an organism growing on Kligler's iron agar ferments glucose, but
NOT lactose, why does the slant ultimately turn red? [ Ans: ]
When the glucose supply is exhausted, the organism turns to
© 2025 Assignment Expert
peptone degradation, releasing alkaline end products.
Which of the following would be appropriately sterilized using UV
radiation? [ Ans: ] stainless steel surface in biological safety
cabinet used for media preparation
Guru01 - Stuvia
Which of the following DNA sequences would be the most likely to
be damaged by UV radiation? [ Ans: ] CTTGAATTAA
Which of the following can determine the particular fermentation
pathway used by a fermenting microorganism? [ Ans: ]
Inoculation of MRVP broth, followed by MR and VP tests
As the concentration of an enzyme increases for a given
substrate, [ Ans: ] the reaction rate increases similarly.
What would you predict would happen to the fermentation rate if
you used yeast suspension that had not been swirled/mixed each
time before it was dispensed into test tubes? [ Ans: ] The reaction
rate would be very low.
Mitochondria are associated with the production of _____. [ Ans:
] ATP
Lab coats, gloves, face masks, and goggles are all examples of [
Ans: ] personal protective equipment
Which of the following substrates is fermented most easily by
yeast? [ Ans: ] glucose
