1
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Microbiology Lab MIDTERM Questions and
Answers (100% Correct Answers) Already
Graded A+
Which of the following items can be washed in the sink after use in
the laboratory? (Select all that apply)
- dissection tools
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- glassware that was not used with hazardous chemicals
- E. coli broth culture tubes
- inoculating loops
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- dissection trays [ Ans: ] - dissection tools
- glassware that was not used with hazardous chemicals
- dissection trays
Indicate the correct order of steps as you bring an object into
focus under high power.
- Rotate to low power objective, then use fine focus to sharpen
the image
- Turn on the light and center the specimen over the light source.
- Place slide on stage and secure with stage clips.
- Readjust fine focus under high power to produce the sharpest
image.
- Focus specimen on scanning objective using coarse focus
adjustment
- Rotate high power lens into position. [ Ans: ] - Place slide on
stage and secure with stage clips.
- Turn on the light and center the specimen over the light source.
, 2
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- Focus specimen on scanning objective using coarse focus
adjustment
- Rotate to low power objective, then use fine focus to sharpen
the image
- Rotate high power lens into position.
- Readjust fine focus under high power to produce the sharpest
image.
Which of the following represent important safety procedures for
microbiology students?
(Select all that apply)
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- Always wear open-toed shoes so organisms do not become
trapped in shoes.
- Wash your hands before and after laboratory procedures.
- Disinfect your lab bench at the beginning and end of the lab
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period.
- Only drink a closed container of water while conducting lab
procedures.
- Take extra care around the flame of a Bunsen burner by tying up
hair and keeping it from combustibles [ Ans: ] - Wash your hands
before and after laboratory procedures.
- Disinfect your lab bench at the beginning and end of the lab
period.
- Take extra care around the flame of a Bunsen burner by tying up
hair and keeping it from combustibles.
T/F You may eat a snack in lab as long as there are no bacterial
cultures on your bench and you have thoroughly washed your
hands. [ Ans: ] False
A disinfectant is used on your work surface:
- after all work is complete.
- All of the answer choices are correct.
- before the beginning of laboratory procedures.
, 3
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- after any spill of live microorganisms. [ Ans: ] All of the answer
choices are correct.
Which of the following items may be on the laboratory bench top
while doing lab work? (Select all that apply)
- cultures (broth tubes, agar plates, agar slants)
- equipment items specifically needed to complete the lab
activity.
- backpack, purse
- pencil or pen
- cell phone [ Ans: ] - cultures (broth tubes, agar plates, agar
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slants)
- equipment items specifically needed to complete the lab
activity.
- pencil or pen
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What should be done to prepare or deal with a laboratory
emergency? (Select all that apply)
- Know the location of safety equipment such as the fire
extinguisher.
- Notify your instructor regarding spills or nonemergency situations.
- Call 911 if an emergency occurs.
- Take antibiotics regularly before attending class in case of spills.
- Conduct all lab procedures under a fume hood. [ Ans: ] - Know
the location of safety equipment such as the fire extinguisher.
- Notify your instructor regarding spills or nonemergency situations.
- Call 911 if an emergency occurs.
Which of the following are reasons for washing hands before and
after lab activities? (Select all that apply)
- Hands may be a source of contamination to the lab
experiments.
, 4
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- Hands may become contaminated with infectious bacteria
during lab experiments.
- Hands may become stained during lab.
- Hands may be contaminated with irritating chemicals during
experiments.
- Hand washing will sterilize hands. [ Ans: ] - Hands may be a
source of contamination to the lab experiments.
- Hands may become contaminated with infectious bacteria
during lab experiments.
- Hands may become stained during lab.
© 2025 Assignment Expert
- Hands may be contaminated with irritating chemicals during
experiments.
How long should hands be lathered while washing? [ Ans: ] 20
seconds
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Which of the following steps are part of a thorough hand washing
protocol? (Select all that apply)
- removal of bracelets, watches, and rings
- applying soap prior to wetting hands
- lathering with soap, rubbing hands together for at least 20
seconds
- using a brush and wooden stick, clean well under finger nails
- using paper towels to turn on the faucet [ Ans: ] - removal of
bracelets, watches, and rings
- lathering with soap, rubbing hands together for at least 20
seconds
- using a brush and wooden stick, clean well under finger nails
Jackie went to the sink at the end of lab to wash her hands. After
removing her watch and rings, she turned on the water, wet her
hands, added soap and thoroughly rubbed and lathered her
hands for 30 seconds, cleaning the backs of her hands, her
fingernails, and between her fingers. She then turned off the
For Expert help and assignment solutions, +254707240657
Microbiology Lab MIDTERM Questions and
Answers (100% Correct Answers) Already
Graded A+
Which of the following items can be washed in the sink after use in
the laboratory? (Select all that apply)
- dissection tools
© 2025 Assignment Expert
- glassware that was not used with hazardous chemicals
- E. coli broth culture tubes
- inoculating loops
Guru01 - Stuvia
- dissection trays [ Ans: ] - dissection tools
- glassware that was not used with hazardous chemicals
- dissection trays
Indicate the correct order of steps as you bring an object into
focus under high power.
- Rotate to low power objective, then use fine focus to sharpen
the image
- Turn on the light and center the specimen over the light source.
- Place slide on stage and secure with stage clips.
- Readjust fine focus under high power to produce the sharpest
image.
- Focus specimen on scanning objective using coarse focus
adjustment
- Rotate high power lens into position. [ Ans: ] - Place slide on
stage and secure with stage clips.
- Turn on the light and center the specimen over the light source.
, 2
For Expert help and assignment solutions, +254707240657
- Focus specimen on scanning objective using coarse focus
adjustment
- Rotate to low power objective, then use fine focus to sharpen
the image
- Rotate high power lens into position.
- Readjust fine focus under high power to produce the sharpest
image.
Which of the following represent important safety procedures for
microbiology students?
(Select all that apply)
© 2025 Assignment Expert
- Always wear open-toed shoes so organisms do not become
trapped in shoes.
- Wash your hands before and after laboratory procedures.
- Disinfect your lab bench at the beginning and end of the lab
Guru01 - Stuvia
period.
- Only drink a closed container of water while conducting lab
procedures.
- Take extra care around the flame of a Bunsen burner by tying up
hair and keeping it from combustibles [ Ans: ] - Wash your hands
before and after laboratory procedures.
- Disinfect your lab bench at the beginning and end of the lab
period.
- Take extra care around the flame of a Bunsen burner by tying up
hair and keeping it from combustibles.
T/F You may eat a snack in lab as long as there are no bacterial
cultures on your bench and you have thoroughly washed your
hands. [ Ans: ] False
A disinfectant is used on your work surface:
- after all work is complete.
- All of the answer choices are correct.
- before the beginning of laboratory procedures.
, 3
For Expert help and assignment solutions, +254707240657
- after any spill of live microorganisms. [ Ans: ] All of the answer
choices are correct.
Which of the following items may be on the laboratory bench top
while doing lab work? (Select all that apply)
- cultures (broth tubes, agar plates, agar slants)
- equipment items specifically needed to complete the lab
activity.
- backpack, purse
- pencil or pen
- cell phone [ Ans: ] - cultures (broth tubes, agar plates, agar
© 2025 Assignment Expert
slants)
- equipment items specifically needed to complete the lab
activity.
- pencil or pen
Guru01 - Stuvia
What should be done to prepare or deal with a laboratory
emergency? (Select all that apply)
- Know the location of safety equipment such as the fire
extinguisher.
- Notify your instructor regarding spills or nonemergency situations.
- Call 911 if an emergency occurs.
- Take antibiotics regularly before attending class in case of spills.
- Conduct all lab procedures under a fume hood. [ Ans: ] - Know
the location of safety equipment such as the fire extinguisher.
- Notify your instructor regarding spills or nonemergency situations.
- Call 911 if an emergency occurs.
Which of the following are reasons for washing hands before and
after lab activities? (Select all that apply)
- Hands may be a source of contamination to the lab
experiments.
, 4
For Expert help and assignment solutions, +254707240657
- Hands may become contaminated with infectious bacteria
during lab experiments.
- Hands may become stained during lab.
- Hands may be contaminated with irritating chemicals during
experiments.
- Hand washing will sterilize hands. [ Ans: ] - Hands may be a
source of contamination to the lab experiments.
- Hands may become contaminated with infectious bacteria
during lab experiments.
- Hands may become stained during lab.
© 2025 Assignment Expert
- Hands may be contaminated with irritating chemicals during
experiments.
How long should hands be lathered while washing? [ Ans: ] 20
seconds
Guru01 - Stuvia
Which of the following steps are part of a thorough hand washing
protocol? (Select all that apply)
- removal of bracelets, watches, and rings
- applying soap prior to wetting hands
- lathering with soap, rubbing hands together for at least 20
seconds
- using a brush and wooden stick, clean well under finger nails
- using paper towels to turn on the faucet [ Ans: ] - removal of
bracelets, watches, and rings
- lathering with soap, rubbing hands together for at least 20
seconds
- using a brush and wooden stick, clean well under finger nails
Jackie went to the sink at the end of lab to wash her hands. After
removing her watch and rings, she turned on the water, wet her
hands, added soap and thoroughly rubbed and lathered her
hands for 30 seconds, cleaning the backs of her hands, her
fingernails, and between her fingers. She then turned off the
