Fanan Feno
10/08/2025
Chang
Organic Chemistry 12A
TLC Lab
Objectives: To identify solvent mixtures that will separate the colored components in
spinach leaves using thin-layer chromatograph
Table of Materials:
Chemical structure Amount Molecular Boiling/Melting Density Hazards
used or Weight Point
produced
Petroleum ether 0.8 mL to 86.18 38°C (100.4°F) 0.64 - ● Highly
grind g/mol (boiling) 0.77 flammable
spinach g/mL ● Highly volatile
● Skin irritation
● Respiratory tract
irritation
Acetone X:30 ratio 58.08 -94°C (-137°F) 0.79 ● Highly
in 2 mL g/mol (melting) g/mL flammable
mixture ● Highly volatile
eluant 56°C (133°F) ● Skin irritation
(boiling) ● Eye irritation
Ethanol 0.4 mL to 46.07 -114°C (-173.2 °F) 0.79 ● Highly volatile
grind g/mol (melting) g/mL ● Flammable
spinach ● Skin irritation
78 °C (172.4°F) ● Eye irritation
(boiling)
Hexane 70:X ratio 86.18 -95 °C (-139 °F) 0.66 ● Flammable
in 2 mL g/mol (melting) g/mL ● Skin irritation
mixture ● Eye irritation
eluant 69 °C (156.2 °F) ● Respiratory
(boiling) distress
● Dizziness or
drowsiness
Anhydrous sodium “Several 142.04 884°C (1623.2 °F) N/A ● Hygroscopic
sulfate spatula-tips g/mol (melting) ● Skin irritation
full” ● Eye irritation
10/08/2025
Chang
Organic Chemistry 12A
TLC Lab
Objectives: To identify solvent mixtures that will separate the colored components in
spinach leaves using thin-layer chromatograph
Table of Materials:
Chemical structure Amount Molecular Boiling/Melting Density Hazards
used or Weight Point
produced
Petroleum ether 0.8 mL to 86.18 38°C (100.4°F) 0.64 - ● Highly
grind g/mol (boiling) 0.77 flammable
spinach g/mL ● Highly volatile
● Skin irritation
● Respiratory tract
irritation
Acetone X:30 ratio 58.08 -94°C (-137°F) 0.79 ● Highly
in 2 mL g/mol (melting) g/mL flammable
mixture ● Highly volatile
eluant 56°C (133°F) ● Skin irritation
(boiling) ● Eye irritation
Ethanol 0.4 mL to 46.07 -114°C (-173.2 °F) 0.79 ● Highly volatile
grind g/mol (melting) g/mL ● Flammable
spinach ● Skin irritation
78 °C (172.4°F) ● Eye irritation
(boiling)
Hexane 70:X ratio 86.18 -95 °C (-139 °F) 0.66 ● Flammable
in 2 mL g/mol (melting) g/mL ● Skin irritation
mixture ● Eye irritation
eluant 69 °C (156.2 °F) ● Respiratory
(boiling) distress
● Dizziness or
drowsiness
Anhydrous sodium “Several 142.04 884°C (1623.2 °F) N/A ● Hygroscopic
sulfate spatula-tips g/mol (melting) ● Skin irritation
full” ● Eye irritation
