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Chapter 4 Bio 1406 Questions with Detailed
Verified Answers
1) When biologists wish to study the internal ultrastructure of cells, they
can achieve the finest
resolution by using a
A) phase-contrast light microscope.
B) scanning electron microscope.
C) transmission electronic microscope.
D) confocal fluorescence microscope. Ans: C transmission electron
microscope
2) One advantage of light microscopy over transmission electron
microscopy is that
A) specimen preparation for light microcopy does not produce artifacts.
B) light microscopy provides for higher resolving power than transmission
electron microscopy.
C) light microscopy provides for higher magnification than transmission
electron microscopy.
D) light microscopy allows you to view dynamic processes in living cells.
Ans: D light microscopy allows you to view dynamic processes in living
cells
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3) Cell fractionation is commonly used in biological research to
A) sort cells based on their size and weight.
B) visualize the 3-D structure of cell membranes.
C) isolate organelles to examine their biological functions.
D) examine the distribution of organelles within the cell. Ans: C Isolate
organelles to examine their biological functions.
4) A researcher disrupts cells and separates subcellular components
using centrifugation. What is the primary factor that determines whether
a specific cellular component remains in the liquid solution or ends up in
the pellet?
A) the relative size and weight of the component
B) the relative solubility of the component
C) how hydrophobic the component is
D) the carbohydrate composition of the component Ans: A the relative
size and weight of the component
5) A researcher disrupts cells and separates subcellular components
using centrifugation. Which of the following correctly lists the order in
which cellular components will pellet when disrupted cell mixtures are
centrifuged at increasingly higher speeds?
A) ribosomes, nuclei, mitochondria
B) chloroplasts, ribosomes, pieces of membrane
C) nucleus, ribosomes, chloroplasts
D) nucleus, mitochondria, ribosomes Ans: D nucleus, mitochondria,
ribosomes
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6) What is the primary reason that a modern transmission electron
microscope (TEM) can resolve biological structures as small as 2 nm,
whereas 200 nm is the limit of resolution for a
light microscope?
A) Diffraction of light by a light microscope distorts the image.
B) Contrast is enhanced by staining with atoms of heavy metal.
C) Electron beams have much shorter wavelengths than visible light.
D) TEM is used to visualize very thin sections of cells. Ans: C Electron
beams have much shorter wavelengths than visible light
7) What technique would be most appropriate to use to observe the
movements of condensed chromosomes during cell division?
A) standard light microscopy
B) a hand lens (magnifying glass)
C) transmission electron microscopy
D) scanning electron microscopy Ans: A) standard light microscopy
8) The smallest cell structures that would most likely be visible with a
standard (not super-resolution) research-grade light microscope are
A) microtubules.
B) chloroplasts.
C) ribosomes.
D) nuclear pores. Ans: B) chloroplasts.
Chapter 4 Bio 1406 Questions with Detailed
Verified Answers
1) When biologists wish to study the internal ultrastructure of cells, they
can achieve the finest
resolution by using a
A) phase-contrast light microscope.
B) scanning electron microscope.
C) transmission electronic microscope.
D) confocal fluorescence microscope. Ans: C transmission electron
microscope
2) One advantage of light microscopy over transmission electron
microscopy is that
A) specimen preparation for light microcopy does not produce artifacts.
B) light microscopy provides for higher resolving power than transmission
electron microscopy.
C) light microscopy provides for higher magnification than transmission
electron microscopy.
D) light microscopy allows you to view dynamic processes in living cells.
Ans: D light microscopy allows you to view dynamic processes in living
cells
, Page | 2
3) Cell fractionation is commonly used in biological research to
A) sort cells based on their size and weight.
B) visualize the 3-D structure of cell membranes.
C) isolate organelles to examine their biological functions.
D) examine the distribution of organelles within the cell. Ans: C Isolate
organelles to examine their biological functions.
4) A researcher disrupts cells and separates subcellular components
using centrifugation. What is the primary factor that determines whether
a specific cellular component remains in the liquid solution or ends up in
the pellet?
A) the relative size and weight of the component
B) the relative solubility of the component
C) how hydrophobic the component is
D) the carbohydrate composition of the component Ans: A the relative
size and weight of the component
5) A researcher disrupts cells and separates subcellular components
using centrifugation. Which of the following correctly lists the order in
which cellular components will pellet when disrupted cell mixtures are
centrifuged at increasingly higher speeds?
A) ribosomes, nuclei, mitochondria
B) chloroplasts, ribosomes, pieces of membrane
C) nucleus, ribosomes, chloroplasts
D) nucleus, mitochondria, ribosomes Ans: D nucleus, mitochondria,
ribosomes
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6) What is the primary reason that a modern transmission electron
microscope (TEM) can resolve biological structures as small as 2 nm,
whereas 200 nm is the limit of resolution for a
light microscope?
A) Diffraction of light by a light microscope distorts the image.
B) Contrast is enhanced by staining with atoms of heavy metal.
C) Electron beams have much shorter wavelengths than visible light.
D) TEM is used to visualize very thin sections of cells. Ans: C Electron
beams have much shorter wavelengths than visible light
7) What technique would be most appropriate to use to observe the
movements of condensed chromosomes during cell division?
A) standard light microscopy
B) a hand lens (magnifying glass)
C) transmission electron microscopy
D) scanning electron microscopy Ans: A) standard light microscopy
8) The smallest cell structures that would most likely be visible with a
standard (not super-resolution) research-grade light microscope are
A) microtubules.
B) chloroplasts.
C) ribosomes.
D) nuclear pores. Ans: B) chloroplasts.
