Quiz_________________?
Pyrimidine -
Answer☑️
One carbon ring
Cytosine, Thymine, Uracil
Quiz_________________?
Purine -
Answer☑️
Two carbon rings
Adenine, Guanine
Quiz_________________?
How are nucleotides joined together? -
Answer☑️
Condensation to form phosphodiester bond
Quiz_________________?
What is the function of mRNA? -
1
, Answer☑️
Carries genetic info out of nucleus
Transcript translated to protein
Quiz_________________?
What is the function of tRNA? -
Answer☑️
Carries aa to ribosome
Anticodon pairs with codon on mRNA strand
Quiz_________________?
What is the function of rRNA? -
Answer☑️
part of ribosome structure
most abundant RNA
coordinated coupling of tRNA to mRNA codons
Quiz_________________?
Feedback inhibition -
Answer☑️
Product of pathway is noncompetitive inhibitor
Binds to allosteric site to slow down rxn b/c too much product
Quiz_________________?
Exonucleases -
Answer☑️
Degrades nucleic acids by removing one terminal nt at a time
Cleaves phosphodiester bond at end of chain
5' --> 3' and 3' --> 5'
2
, Quiz_________________?
Endonucleases (Prok) -
Answer☑️
Restriction enzymes
Cleaves phoshpodiester bonds w/i poly-nt chain
Recognition site is palindromic sequence
Types I-V
Quiz_________________?
ORI sites -
Answer☑️
nt sequence where replication is initiated
Quiz_________________?
Topoisomerase I -
Answer☑️
Induces ss breaks
Remove DNA supercoils during TXN and DNA replication; for strand breakage during recombination;
for chr condensation; and to disentangle intertwined DNA during mitosis
Quiz_________________?
topoisomerase II -
Answer☑️
cuts both strands of one DNA double helix, passes another unbroken DNA helix through it, and then
reanneals the cut strands
Quiz_________________?
Gyrase (topoisomerase II) -
Answer☑️
3
, Unwinds supercoiling caused by unwinding at the rep fork by introducing DSBs
Quiz_________________?
Helicase -
Answer☑️
Breaks H-bonds of double helix at the replication fork
Quiz_________________?
Primase -
Answer☑️
DNApol α (DNA dep RNA pol)
adds short segments of complementary
RNA to ssDNA template (primers), serves as starting points for replication
Quiz_________________?
single-strand DNA binding proteins (SSBPs) -
Answer☑️
Binds ssDNA and prevents it from re-annealing during TXN, replication, repair, and recombination
Quiz_________________?
Okazaki fragments -
Answer☑️
Short fragments of DNA synthesized by DNApol δ using the lagging strand (3'->5') as a template
Quiz_________________?
Ligase -
Answer☑️
Closes gaps in DNA
Catalyzes phosphodiester bond between 3'OH and 5'P
4
Pyrimidine -
Answer☑️
One carbon ring
Cytosine, Thymine, Uracil
Quiz_________________?
Purine -
Answer☑️
Two carbon rings
Adenine, Guanine
Quiz_________________?
How are nucleotides joined together? -
Answer☑️
Condensation to form phosphodiester bond
Quiz_________________?
What is the function of mRNA? -
1
, Answer☑️
Carries genetic info out of nucleus
Transcript translated to protein
Quiz_________________?
What is the function of tRNA? -
Answer☑️
Carries aa to ribosome
Anticodon pairs with codon on mRNA strand
Quiz_________________?
What is the function of rRNA? -
Answer☑️
part of ribosome structure
most abundant RNA
coordinated coupling of tRNA to mRNA codons
Quiz_________________?
Feedback inhibition -
Answer☑️
Product of pathway is noncompetitive inhibitor
Binds to allosteric site to slow down rxn b/c too much product
Quiz_________________?
Exonucleases -
Answer☑️
Degrades nucleic acids by removing one terminal nt at a time
Cleaves phosphodiester bond at end of chain
5' --> 3' and 3' --> 5'
2
, Quiz_________________?
Endonucleases (Prok) -
Answer☑️
Restriction enzymes
Cleaves phoshpodiester bonds w/i poly-nt chain
Recognition site is palindromic sequence
Types I-V
Quiz_________________?
ORI sites -
Answer☑️
nt sequence where replication is initiated
Quiz_________________?
Topoisomerase I -
Answer☑️
Induces ss breaks
Remove DNA supercoils during TXN and DNA replication; for strand breakage during recombination;
for chr condensation; and to disentangle intertwined DNA during mitosis
Quiz_________________?
topoisomerase II -
Answer☑️
cuts both strands of one DNA double helix, passes another unbroken DNA helix through it, and then
reanneals the cut strands
Quiz_________________?
Gyrase (topoisomerase II) -
Answer☑️
3
, Unwinds supercoiling caused by unwinding at the rep fork by introducing DSBs
Quiz_________________?
Helicase -
Answer☑️
Breaks H-bonds of double helix at the replication fork
Quiz_________________?
Primase -
Answer☑️
DNApol α (DNA dep RNA pol)
adds short segments of complementary
RNA to ssDNA template (primers), serves as starting points for replication
Quiz_________________?
single-strand DNA binding proteins (SSBPs) -
Answer☑️
Binds ssDNA and prevents it from re-annealing during TXN, replication, repair, and recombination
Quiz_________________?
Okazaki fragments -
Answer☑️
Short fragments of DNA synthesized by DNApol δ using the lagging strand (3'->5') as a template
Quiz_________________?
Ligase -
Answer☑️
Closes gaps in DNA
Catalyzes phosphodiester bond between 3'OH and 5'P
4
