ASCP MB Exam Prep: Practice Questions for Medical Laboratory Certificatio
Anti-codon
Thymine
HER2, ERBB2, BRCA1
1. Which of the following is not a component of a nu-
cleotide?
Phosphate group
Anti-codon
Ribose sugar
Nitrogen base
2. According to Chargaff's rule of base pairing, adenine
pairs with:
3. What genes would be screened in a breast cancer
panel?
4. Next Generation Sequencing uses: Short sequence reads
5. Purines and pyrimidines differ from each other in that: Purines have two rings;
pyrimidines have one ring
6. The purines are: T
Cytosine and uracil h
Adenine and thymine y
, ASCP MB Exam Prep: Practice Questions for Medical Laboratory Certificatio
mine and cytosine Adenine and guanine
Adenine and guanine
7. What is the rate of mutation per round of DNA repli-
cation?
1 in 1,000 base pairs
1 in 10,000 base pairs 1 in 1,000,000,000 base
1 in 1,000,000 base pairs pairs
1 in 1,000,000,000 base pairs
8. The rate of DNA migration through an agarose gel
during electrophoresis does not depend on which of
Nucleotide sequence of
the molecule
, ASCP MB Exam Prep: Practice Questions for Medical Laboratory Certificatio
the following factors?
Net charge of the molecule
Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule
9. What are the phases in a qPCR Amplification Plot? Initiation, exponential,
Initiation, exponential, plateau plateau
Baseline, exponential, plateau
Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau
10. Find the palindrome in this restriction enzyme site: 3'-GAC
5'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC
11. A patient with impaired judgment, personality 85 CAG repeats in the
changes, signs of abnormal body movements and Huntington gene
depression comes to the physician's office for a fol-
low-up visit. The physician suspects a single-gene dis-
order may be the cause of those clinical manifesta-
tions. A blood specimen was then sent to your clinical
laboratory for mutation screening in the Huntington
gene. Testing with standard PCR indicates that patient
has Huntington Disease, HD. Which of the following
would be consistent with this diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
, ASCP MB Exam Prep: Practice Questions for Medical Laboratory Certificatio
25 CGA repeats in the Huntington gene
85 CGA repeats in the Huntington gene
12. Which two HPV types are responsible for most cases 16 and 18
of cervical cancer?
16 and 18
31 and 59
16 and 58
44 and 59
13. Replication forks, known as origins of DNA replication, Helicase
are created by this enzyme:
Ligase
Taq Polymerase
Primase
Helicase
14. Mutation in what gene is associated with Fragile X FMR1
syndrome?
15. Mantle cell lymphoma (MCL) is caused by what t(11;14)
translocation?
16. This polymerase is involved in "initiation of DNA repli- Pol α
cation and has primase activity":
17. Its discovery shed light on why there is simultaneous, Okazaki fragments
though not continuous, synthesis of DNA on both
leading and lagging strands of DNA:
Klenow fragment of DNA polymerase
Okazaki fragments
Sanger fragments
Anti-codon
Thymine
HER2, ERBB2, BRCA1
1. Which of the following is not a component of a nu-
cleotide?
Phosphate group
Anti-codon
Ribose sugar
Nitrogen base
2. According to Chargaff's rule of base pairing, adenine
pairs with:
3. What genes would be screened in a breast cancer
panel?
4. Next Generation Sequencing uses: Short sequence reads
5. Purines and pyrimidines differ from each other in that: Purines have two rings;
pyrimidines have one ring
6. The purines are: T
Cytosine and uracil h
Adenine and thymine y
, ASCP MB Exam Prep: Practice Questions for Medical Laboratory Certificatio
mine and cytosine Adenine and guanine
Adenine and guanine
7. What is the rate of mutation per round of DNA repli-
cation?
1 in 1,000 base pairs
1 in 10,000 base pairs 1 in 1,000,000,000 base
1 in 1,000,000 base pairs pairs
1 in 1,000,000,000 base pairs
8. The rate of DNA migration through an agarose gel
during electrophoresis does not depend on which of
Nucleotide sequence of
the molecule
, ASCP MB Exam Prep: Practice Questions for Medical Laboratory Certificatio
the following factors?
Net charge of the molecule
Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule
9. What are the phases in a qPCR Amplification Plot? Initiation, exponential,
Initiation, exponential, plateau plateau
Baseline, exponential, plateau
Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau
10. Find the palindrome in this restriction enzyme site: 3'-GAC
5'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC
11. A patient with impaired judgment, personality 85 CAG repeats in the
changes, signs of abnormal body movements and Huntington gene
depression comes to the physician's office for a fol-
low-up visit. The physician suspects a single-gene dis-
order may be the cause of those clinical manifesta-
tions. A blood specimen was then sent to your clinical
laboratory for mutation screening in the Huntington
gene. Testing with standard PCR indicates that patient
has Huntington Disease, HD. Which of the following
would be consistent with this diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
, ASCP MB Exam Prep: Practice Questions for Medical Laboratory Certificatio
25 CGA repeats in the Huntington gene
85 CGA repeats in the Huntington gene
12. Which two HPV types are responsible for most cases 16 and 18
of cervical cancer?
16 and 18
31 and 59
16 and 58
44 and 59
13. Replication forks, known as origins of DNA replication, Helicase
are created by this enzyme:
Ligase
Taq Polymerase
Primase
Helicase
14. Mutation in what gene is associated with Fragile X FMR1
syndrome?
15. Mantle cell lymphoma (MCL) is caused by what t(11;14)
translocation?
16. This polymerase is involved in "initiation of DNA repli- Pol α
cation and has primase activity":
17. Its discovery shed light on why there is simultaneous, Okazaki fragments
though not continuous, synthesis of DNA on both
leading and lagging strands of DNA:
Klenow fragment of DNA polymerase
Okazaki fragments
Sanger fragments
