BIMM 101 Quiz 1 questions well
answered to pass
What does X% concentration mean? - correct answer ✔✔ Xg/100mL OR
XmL/Total mL
DF=? - correct answer ✔✔ Vi/Vf
OR
Cf/Ci
How to find total Dilution factor? - correct answer ✔✔ Multiply all DF's together
How to find final concentration? - correct answer ✔✔ DF times Ci
What should be included in flow charts? - correct answer ✔✔ Outline, Protocol changes,
Hypothesis
What should be included in Results? - correct answer ✔✔ Analysis, Hypothesis results, Compare
Data, Troubleshoot, Uncertainty, Experiment and Control.
What is a gene? - correct answer ✔✔ A section of DNA that codes for a protein
What direction does RNA pol transcribes? - correct answer ✔✔ 5' to 3'
What is RNA polymerase and what does it do? - correct answer ✔✔ RNA polymerase binds to
DNA Promoter during transcription and separates the DNA strands
, What is a promoter? - correct answer ✔✔ binding site for RNA polymerase, starts transcription
Where can you find exons and introns? - correct answer ✔✔ Only in Eukaryotes DNA, but
NEVER in processed mRNA
What is UTR? - correct answer ✔✔ Region of the processed mRNA that is NOT translated.
Can have other functions such as attachment during translation
What is open reading frame? - correct answer ✔✔ Sequence of mRNA that actually codes for a
polypeptide. (OPPOSITE OF UTR).
What is the termination sequence? - correct answer ✔✔ A sequence on the DNA that stops
transcription
What is purpose of Agarose Gel Electrophoresis? - correct answer ✔✔ Determine size OR
amount of DNA fragments. Also can purify DNA fragments.
What is Agarose? - correct answer ✔✔ component of agar, a complex polysaccharide from
seaweed.
How does DNA migrate on the gel? - correct answer ✔✔ They have negative charged phosphate
groups put under the current of the buffer.
What is SYBR Safe? - correct answer ✔✔ Intercalating Agent with fluorescent dye (under UV). To
help visualize DNA on the gel. Also is proportional to Total mass of DNA.
It has many rings for resonance that are similar to base pairs.
answered to pass
What does X% concentration mean? - correct answer ✔✔ Xg/100mL OR
XmL/Total mL
DF=? - correct answer ✔✔ Vi/Vf
OR
Cf/Ci
How to find total Dilution factor? - correct answer ✔✔ Multiply all DF's together
How to find final concentration? - correct answer ✔✔ DF times Ci
What should be included in flow charts? - correct answer ✔✔ Outline, Protocol changes,
Hypothesis
What should be included in Results? - correct answer ✔✔ Analysis, Hypothesis results, Compare
Data, Troubleshoot, Uncertainty, Experiment and Control.
What is a gene? - correct answer ✔✔ A section of DNA that codes for a protein
What direction does RNA pol transcribes? - correct answer ✔✔ 5' to 3'
What is RNA polymerase and what does it do? - correct answer ✔✔ RNA polymerase binds to
DNA Promoter during transcription and separates the DNA strands
, What is a promoter? - correct answer ✔✔ binding site for RNA polymerase, starts transcription
Where can you find exons and introns? - correct answer ✔✔ Only in Eukaryotes DNA, but
NEVER in processed mRNA
What is UTR? - correct answer ✔✔ Region of the processed mRNA that is NOT translated.
Can have other functions such as attachment during translation
What is open reading frame? - correct answer ✔✔ Sequence of mRNA that actually codes for a
polypeptide. (OPPOSITE OF UTR).
What is the termination sequence? - correct answer ✔✔ A sequence on the DNA that stops
transcription
What is purpose of Agarose Gel Electrophoresis? - correct answer ✔✔ Determine size OR
amount of DNA fragments. Also can purify DNA fragments.
What is Agarose? - correct answer ✔✔ component of agar, a complex polysaccharide from
seaweed.
How does DNA migrate on the gel? - correct answer ✔✔ They have negative charged phosphate
groups put under the current of the buffer.
What is SYBR Safe? - correct answer ✔✔ Intercalating Agent with fluorescent dye (under UV). To
help visualize DNA on the gel. Also is proportional to Total mass of DNA.
It has many rings for resonance that are similar to base pairs.
