BIMM 101 questions well answered
graded A+ updated
Goals for BIMM 101 - correct answer ✔✔ Apply knowledge of molecular biology concepts and
molecular techniques to plan experiments, explain and troubleshoot results.
Demonstrate proficiency at the basic molcular biology techniques used in the lab.
Explain the importance of proper controls in designing experiments and interpreting results.
Perform basic lab math skills, statistical analysis, and graphing.
Draw conclusions based on evidence and reasoning.
Use basic bioinformatics databases and applications.
Find, read, and evaluate primary literature.
Collaborate with one another to learn foundational biological concepts and labratory skills.
Projects and skill development - correct answer ✔✔ Dilutions
Agaraose Gel Electrophoresis
Site-directed mutagenesis: effect of regulatory element mutations on gene expression in
bacteria.
Gene expression and phenotype: RNAi in C. Elegans
Genotype-Phenotype: alleles
Bacterial Promoters - correct answer ✔✔ Contains two short bp sequences where in between is
the location where you can introduce a new mutation.
Plasmids - correct answer ✔✔ Circular components of DNA, they most often exist apart from
the chromosome, during bacterial reproduction they are duplicated and passed on to offspring,
they often confer protective traits such as resisting drugs and producing toxins or enzymes and
can be used in replication to make many copies of a protein of interest.
, Dilutions - correct answer ✔✔ c₁v₁=c₂v₂
Dilution factor: c₁/c₂ or v₂/v₁
Serial dilution, making an original dilution then continuing the dilutions.
Agarose Gel - correct answer ✔✔ We separate DNA fragments based on size and amount. Size is
based on how far the band has migrated and amount is based on the intensity of the band. Gels
of different percentages of agarose can vary to accommodate the different sizes of DNA
fragments.
Visualizing DNA - correct answer ✔✔ SyberSafe intercalates between adjacent base pairs in
DNA. The resulting DNA-dye-complex absorbs blue light (lambdamax= 509nm) and emits green
light (lamda max=524nm). Sybersafe is positively charge, is attracted to the DNA
Components of gel electrophoresis: - correct answer ✔✔ 1. DNA runs in electromagnetic field
2. The gel consists of agarose is dissolved in TAE buffer
(Tris-acetate-EDTA). EDTA chelates magnesium,
reducing the ability of enzymes - such as nucleases - to
function.
3. During the electrophoresis, the gel is immersed in TAE
buffer
4. DNA fragments are separated based on their size
How do mutations in the promoter/RBS affect expression? - correct answer ✔✔ Extract plasmid
DNA→Digest P1 plasmid to open, Digest RFP out of plasmid→Ligate RFP into P1 plasmid = P1-
RFP→ Use as template to make mutated sequence using PCR-based mutagenesis→Use PCR to
introduce a specific mutation→Isolate Pm-RFP plasmid→Transform into E.coli→Bacteria will
take up plasmid and replicate→Isolate and sequence Pm-RFP
Finally analyze sequence to confirm mutation is correct
graded A+ updated
Goals for BIMM 101 - correct answer ✔✔ Apply knowledge of molecular biology concepts and
molecular techniques to plan experiments, explain and troubleshoot results.
Demonstrate proficiency at the basic molcular biology techniques used in the lab.
Explain the importance of proper controls in designing experiments and interpreting results.
Perform basic lab math skills, statistical analysis, and graphing.
Draw conclusions based on evidence and reasoning.
Use basic bioinformatics databases and applications.
Find, read, and evaluate primary literature.
Collaborate with one another to learn foundational biological concepts and labratory skills.
Projects and skill development - correct answer ✔✔ Dilutions
Agaraose Gel Electrophoresis
Site-directed mutagenesis: effect of regulatory element mutations on gene expression in
bacteria.
Gene expression and phenotype: RNAi in C. Elegans
Genotype-Phenotype: alleles
Bacterial Promoters - correct answer ✔✔ Contains two short bp sequences where in between is
the location where you can introduce a new mutation.
Plasmids - correct answer ✔✔ Circular components of DNA, they most often exist apart from
the chromosome, during bacterial reproduction they are duplicated and passed on to offspring,
they often confer protective traits such as resisting drugs and producing toxins or enzymes and
can be used in replication to make many copies of a protein of interest.
, Dilutions - correct answer ✔✔ c₁v₁=c₂v₂
Dilution factor: c₁/c₂ or v₂/v₁
Serial dilution, making an original dilution then continuing the dilutions.
Agarose Gel - correct answer ✔✔ We separate DNA fragments based on size and amount. Size is
based on how far the band has migrated and amount is based on the intensity of the band. Gels
of different percentages of agarose can vary to accommodate the different sizes of DNA
fragments.
Visualizing DNA - correct answer ✔✔ SyberSafe intercalates between adjacent base pairs in
DNA. The resulting DNA-dye-complex absorbs blue light (lambdamax= 509nm) and emits green
light (lamda max=524nm). Sybersafe is positively charge, is attracted to the DNA
Components of gel electrophoresis: - correct answer ✔✔ 1. DNA runs in electromagnetic field
2. The gel consists of agarose is dissolved in TAE buffer
(Tris-acetate-EDTA). EDTA chelates magnesium,
reducing the ability of enzymes - such as nucleases - to
function.
3. During the electrophoresis, the gel is immersed in TAE
buffer
4. DNA fragments are separated based on their size
How do mutations in the promoter/RBS affect expression? - correct answer ✔✔ Extract plasmid
DNA→Digest P1 plasmid to open, Digest RFP out of plasmid→Ligate RFP into P1 plasmid = P1-
RFP→ Use as template to make mutated sequence using PCR-based mutagenesis→Use PCR to
introduce a specific mutation→Isolate Pm-RFP plasmid→Transform into E.coli→Bacteria will
take up plasmid and replicate→Isolate and sequence Pm-RFP
Finally analyze sequence to confirm mutation is correct
