National Phlebotomy Certification
Exam 2025
An individual who has little resistance to an infectious microbe is referred to as a
susceptible what? - Correct Ans-Host
Which of the following is an example of a work practice control that reduces risk of
exposure to bloodborne pathogens? - Correct Ans-Wearing gloves to draw blood
This ion is essential to the coagulation process. - Correct Ans-Calcium
QC protocols prohibit use of outdated evacuated tubes because why? - Correct Ans-
Tubes my not fill completely, changing additive-to-sample ratios.
Molecular genetic testing requires what? - Correct Ans-Specimens to be collected in
sterile EDTA.
What is the purpose of an antiglycolytic agent? - Correct Ans-Preserve glucose
Which on of the following tubes is filled first when multiple tubes are filled using an
evacuated tube system? - Correct Ans-Blood culture (SPS) tube
What is the recommended disinfectant for blood culture sites in infants 2 months and
older? - Correct Ans-Chlorhexidine gluconate
Latent fibrin formation in serum can result from what? - Correct Ans-Incomplete clotting
when centrifuged
In which of the following areas does capillary specimen collection differ from routine
venipuncture when collecting a BUN and CBC? - Correct Ans-Order of draw
This is an example of a preanalytical error made at the time of collection. - Correct Ans-
Failing to mix tubes
What constitutes a positive modified Allen test? - Correct Ans-The hand color returns to
normal in 15 seconds.
According to CLSI, which tubes should be placed upright as soon as they are mixed? -
Correct Ans-Nonanticoagulant gel tubes
Reference values are higher for capillary specimens with what test? - Correct Ans-
Glucose
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According to CLSI, the maximum time limit for separating serum or plasma from cells is
how long? - Correct Ans-2.0 hours from time of collection
All of the following are a reason for transporting tubes with the stoppers up EXCEPT
which of the following? - Correct Ans-Maintains the sterility of the sample.
The liquid portion of a clotted specimen is called what? - Correct Ans-Serum
The most common reason for glucose monitoring through POCT is to what? - Correct
Ans-Monitor glucose levels for diabetic care.
The AMT, ACA, and ASCP are agencies that do what? - Correct Ans-Certify laboratory
professionals
A quadriplegic patient can do what of the following? - Correct Ans-Can feel pain if there
is no sensory damage.
The standard of care used in phlebotomy malpractice cases is often based on
guidelines from what organization? - Correct Ans-CLSI - Clinical and Laboratory
Standards Institute
In collecting blood cultures, one of the most frequent errors made is what? - Correct
Ans-Improper cleansing of the collection site
Which type of specimen is typically used for routine urinalysis? - Correct Ans-Random
A positive FIT test indicates what? - Correct Ans-Bleeding in the colon or rectum
Accessioning a specimen involves what? - Correct Ans-AOTA-Connecting it with the
correct individual, giving it a unique identification number, and recording it in the order it
was received.
A blood specimen collected in nongel tube has been centrifuged. The cells at the
bottom are free flowing and can be resuspended if the tube is inverted. The term used
to describe the liquid portion on top of the cells is what? - Correct Ans-Plasma
The composition of capillary puncture blood more closely resembles what? - Correct
Ans-Arterial Blood
A phlebotomist arrives to collect a 2-hour post-prandial glucose specimen on an
inpatient and discovers that 2 hours have not elapsed since the patient's meal. What
should the phlebotomist do? - Correct Ans-Ask the patient's nurse to verify the correct
time to draw the specimen.
Which of the following is referred to as a delivering chamber of the heart? - Correct Ans-
Left ventricle
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