Thin Layer Chromatography ACTUAL
UPDATED Exam Questions and CORRECT
Answers
Thin Layer Chromatography (TLC) - CORRECT ANSWER - Analytical technique
separating components within a mixture.
Quantifying Compounds - CORRECT ANSWER - Using TLC to determine quantity and
identity of compounds in a reaction mixture.
Monitoring Reactions - CORRECT ANSWER - Using TLC to monitor the progress of a
reaction.
Chromatography - CORRECT ANSWER - Separation of sample components based on
interactions between stationary and mobile phases.
Stationary Phase - CORRECT ANSWER - 3D silica gel network with polar surface,
interacts with sample via intermolecular forces.
Mobile Phase - CORRECT ANSWER - Solvent competing with binding sites of the plate,
pushes samples up the plate.
Affinity - CORRECT ANSWER - Dependent on interaction strength, determines speed of
migrating sample component.
Elution - CORRECT ANSWER - Process of a component being washed out of the
stationary phase by the mobile phase.
UV Visualization - CORRECT ANSWER - Using UV light to visualize organic
compounds on the TLC plate.
UPDATED Exam Questions and CORRECT
Answers
Thin Layer Chromatography (TLC) - CORRECT ANSWER - Analytical technique
separating components within a mixture.
Quantifying Compounds - CORRECT ANSWER - Using TLC to determine quantity and
identity of compounds in a reaction mixture.
Monitoring Reactions - CORRECT ANSWER - Using TLC to monitor the progress of a
reaction.
Chromatography - CORRECT ANSWER - Separation of sample components based on
interactions between stationary and mobile phases.
Stationary Phase - CORRECT ANSWER - 3D silica gel network with polar surface,
interacts with sample via intermolecular forces.
Mobile Phase - CORRECT ANSWER - Solvent competing with binding sites of the plate,
pushes samples up the plate.
Affinity - CORRECT ANSWER - Dependent on interaction strength, determines speed of
migrating sample component.
Elution - CORRECT ANSWER - Process of a component being washed out of the
stationary phase by the mobile phase.
UV Visualization - CORRECT ANSWER - Using UV light to visualize organic
compounds on the TLC plate.